CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans
Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how t...
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| Veröffentlicht in: | Molecular biology of the cell 2011-07, Vol.22 (14), p.2458-2469 |
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| container_title | Molecular biology of the cell |
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| creator | Gutiérrez-Escribano, Pilar González-Novo, Alberto Suárez, M Belén Li, Chang-Run Wang, Yue de Aldana, Carlos R Vázquez Correa-Bordes, Jaime |
| description | Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are regulated. In Candida albicans, the cyclin-dependent kinase (CDK) Cdc28 and the NDR kinase Cbk1 are required for hyphal growth. Here we demonstrate that Mob2, the Cbk1 activator, undergoes a Cdc28-dependent differential phosphorylation on hyphal induction. Mutations in the four CDK consensus sites in Mob2 to Ala significantly impaired hyphal development. The mutant cells produced short hyphae with enlarged tips that displayed an illicit activation of cell separation. We also show that Cdc28 phosphorylation of Mob2 is essential for the maintenance of polarisome components at hyphal tips but not at bud tips during yeast growth. Thus we have found a novel signaling pathway by which Cdc28 controls Cbk1 through the regulatory phosphorylation of Mob2, which is crucial for normal hyphal development. |
| doi_str_mv | 10.1091/mbc.E11-03-0205 |
| format | Article |
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For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are regulated. In Candida albicans, the cyclin-dependent kinase (CDK) Cdc28 and the NDR kinase Cbk1 are required for hyphal growth. Here we demonstrate that Mob2, the Cbk1 activator, undergoes a Cdc28-dependent differential phosphorylation on hyphal induction. Mutations in the four CDK consensus sites in Mob2 to Ala significantly impaired hyphal development. The mutant cells produced short hyphae with enlarged tips that displayed an illicit activation of cell separation. We also show that Cdc28 phosphorylation of Mob2 is essential for the maintenance of polarisome components at hyphal tips but not at bud tips during yeast growth. Thus we have found a novel signaling pathway by which Cdc28 controls Cbk1 through the regulatory phosphorylation of Mob2, which is crucial for normal hyphal development.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.E11-03-0205</identifier><identifier>PMID: 21593210</identifier><language>eng</language><publisher>United States: The American Society for Cell Biology</publisher><subject>Amino Acid Sequence ; Candida albicans ; Candida albicans - genetics ; Candida albicans - growth & development ; Candida albicans - metabolism ; Cell activation ; Cell cycle ; Cell Cycle - genetics ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; Cyclin-dependent kinase ; Cyclin-Dependent Kinases - genetics ; Cyclin-Dependent Kinases - metabolism ; Development ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Hyphae ; Hyphae - genetics ; Hyphae - growth & development ; Hyphae - metabolism ; Hyphal tips ; Mob protein ; Molecular Sequence Data ; Morphogenesis ; Mutation ; Ndr protein ; Phosphorylation ; Phosphorylation - genetics ; Sequence Homology, Amino Acid ; Signal transduction ; Signal Transduction - genetics</subject><ispartof>Molecular biology of the cell, 2011-07, Vol.22 (14), p.2458-2469</ispartof><rights>2011 Gutiérrez-Escribano This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License ( ). 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-9056f8de3fade32ec35d0f5c90c875c85b02679653341b0caae41d58cd3287033</citedby><cites>FETCH-LOGICAL-c430t-9056f8de3fade32ec35d0f5c90c875c85b02679653341b0caae41d58cd3287033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135472/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135472/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21593210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lew, Daniel</contributor><creatorcontrib>Gutiérrez-Escribano, Pilar</creatorcontrib><creatorcontrib>González-Novo, Alberto</creatorcontrib><creatorcontrib>Suárez, M Belén</creatorcontrib><creatorcontrib>Li, Chang-Run</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>de Aldana, Carlos R Vázquez</creatorcontrib><creatorcontrib>Correa-Bordes, Jaime</creatorcontrib><title>CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are regulated. In Candida albicans, the cyclin-dependent kinase (CDK) Cdc28 and the NDR kinase Cbk1 are required for hyphal growth. Here we demonstrate that Mob2, the Cbk1 activator, undergoes a Cdc28-dependent differential phosphorylation on hyphal induction. Mutations in the four CDK consensus sites in Mob2 to Ala significantly impaired hyphal development. The mutant cells produced short hyphae with enlarged tips that displayed an illicit activation of cell separation. We also show that Cdc28 phosphorylation of Mob2 is essential for the maintenance of polarisome components at hyphal tips but not at bud tips during yeast growth. Thus we have found a novel signaling pathway by which Cdc28 controls Cbk1 through the regulatory phosphorylation of Mob2, which is crucial for normal hyphal development.</description><subject>Amino Acid Sequence</subject><subject>Candida albicans</subject><subject>Candida albicans - genetics</subject><subject>Candida albicans - growth & development</subject><subject>Candida albicans - metabolism</subject><subject>Cell activation</subject><subject>Cell cycle</subject><subject>Cell Cycle - genetics</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cyclin-dependent kinase</subject><subject>Cyclin-Dependent Kinases - genetics</subject><subject>Cyclin-Dependent Kinases - metabolism</subject><subject>Development</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Hyphae</subject><subject>Hyphae - genetics</subject><subject>Hyphae - growth & development</subject><subject>Hyphae - metabolism</subject><subject>Hyphal tips</subject><subject>Mob protein</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>Mutation</subject><subject>Ndr protein</subject><subject>Phosphorylation</subject><subject>Phosphorylation - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal transduction</subject><subject>Signal Transduction - genetics</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctP3DAQxi3UiveZW-VbewnM-BE7FyS0BVqVqhc4Isuxna5REod4F2n_e7yCIrj0MA9pfvNpRh8hJwinCA2eDa07vUSsgFfAQO6QfWx4Uwmp60-lB9lUKJnYIwc5PwCgELXaJXsMZcMZwj65X3z_VfkwhdGHcUWnZcol5k1vVzGNNHX0d2oZjZmGnAsRbU-7NNPlZlqW1oen0Kdp2O7GkS7s6KO31PZtdHbMR-RzZ_scjl_rIbm7urxd_Khu_lz_XFzcVE5wWFUNyLrTPvDOlsSC49JDJ10DTivptGyB1aqpJecCW3DWBoFeauc50wo4PyTnL7rTuh2Cd-Wc2fZmmuNg541JNpqPkzEuzd_0ZDhyKRQrAl9fBeb0uA55ZYaYXeh7O4a0zkYrJUAzVIX89l8SgWlQWkpZ0LMX1M0p5zl0bwchmK19pthnAqIBbrb2lY0v7_944__5xZ8BbPmXYQ</recordid><startdate>20110715</startdate><enddate>20110715</enddate><creator>Gutiérrez-Escribano, Pilar</creator><creator>González-Novo, Alberto</creator><creator>Suárez, M Belén</creator><creator>Li, Chang-Run</creator><creator>Wang, Yue</creator><creator>de Aldana, Carlos R Vázquez</creator><creator>Correa-Bordes, Jaime</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110715</creationdate><title>CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans</title><author>Gutiérrez-Escribano, Pilar ; González-Novo, Alberto ; Suárez, M Belén ; Li, Chang-Run ; Wang, Yue ; de Aldana, Carlos R Vázquez ; Correa-Bordes, Jaime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-9056f8de3fade32ec35d0f5c90c875c85b02679653341b0caae41d58cd3287033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Sequence</topic><topic>Candida albicans</topic><topic>Candida albicans - genetics</topic><topic>Candida albicans - growth & development</topic><topic>Candida albicans - metabolism</topic><topic>Cell activation</topic><topic>Cell cycle</topic><topic>Cell Cycle - genetics</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cyclin-dependent kinase</topic><topic>Cyclin-Dependent Kinases - genetics</topic><topic>Cyclin-Dependent Kinases - metabolism</topic><topic>Development</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Hyphae</topic><topic>Hyphae - genetics</topic><topic>Hyphae - growth & development</topic><topic>Hyphae - metabolism</topic><topic>Hyphal tips</topic><topic>Mob protein</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>Mutation</topic><topic>Ndr protein</topic><topic>Phosphorylation</topic><topic>Phosphorylation - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal transduction</topic><topic>Signal Transduction - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez-Escribano, Pilar</creatorcontrib><creatorcontrib>González-Novo, Alberto</creatorcontrib><creatorcontrib>Suárez, M Belén</creatorcontrib><creatorcontrib>Li, Chang-Run</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>de Aldana, Carlos R Vázquez</creatorcontrib><creatorcontrib>Correa-Bordes, Jaime</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gutiérrez-Escribano, Pilar</au><au>González-Novo, Alberto</au><au>Suárez, M Belén</au><au>Li, Chang-Run</au><au>Wang, Yue</au><au>de Aldana, Carlos R Vázquez</au><au>Correa-Bordes, Jaime</au><au>Lew, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2011-07-15</date><risdate>2011</risdate><volume>22</volume><issue>14</issue><spage>2458</spage><epage>2469</epage><pages>2458-2469</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><abstract>Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are regulated. In Candida albicans, the cyclin-dependent kinase (CDK) Cdc28 and the NDR kinase Cbk1 are required for hyphal growth. Here we demonstrate that Mob2, the Cbk1 activator, undergoes a Cdc28-dependent differential phosphorylation on hyphal induction. Mutations in the four CDK consensus sites in Mob2 to Ala significantly impaired hyphal development. The mutant cells produced short hyphae with enlarged tips that displayed an illicit activation of cell separation. We also show that Cdc28 phosphorylation of Mob2 is essential for the maintenance of polarisome components at hyphal tips but not at bud tips during yeast growth. Thus we have found a novel signaling pathway by which Cdc28 controls Cbk1 through the regulatory phosphorylation of Mob2, which is crucial for normal hyphal development.</abstract><cop>United States</cop><pub>The American Society for Cell Biology</pub><pmid>21593210</pmid><doi>10.1091/mbc.E11-03-0205</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Candida albicans Candida albicans - genetics Candida albicans - growth & development Candida albicans - metabolism Cell activation Cell cycle Cell Cycle - genetics Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cyclin-dependent kinase Cyclin-Dependent Kinases - genetics Cyclin-Dependent Kinases - metabolism Development Fungal Proteins - genetics Fungal Proteins - metabolism Hyphae Hyphae - genetics Hyphae - growth & development Hyphae - metabolism Hyphal tips Mob protein Molecular Sequence Data Morphogenesis Mutation Ndr protein Phosphorylation Phosphorylation - genetics Sequence Homology, Amino Acid Signal transduction Signal Transduction - genetics |
| title | CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans |
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