Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens

Functional two-photon Ca(2+)-imaging is a versatile tool to study the dynamics of neuronal populations in brain slices and living animals. However, population imaging is typically restricted to a single two-dimensional image plane. By introducing an electrically tunable lens into the excitation path...

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Veröffentlicht in:Biomedical optics express 2011-07, Vol.2 (7), p.2035-2046
Hauptverfasser: Grewe, Benjamin F, Voigt, Fabian F, van 't Hoff, Marcel, Helmchen, Fritjof
Format: Artikel
Sprache:eng
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