Polystyrene nanoparticle trafficking across MDCK-II
Abstract Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium...
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| Veröffentlicht in: | Nanomedicine 2011-10, Vol.7 (5), p.588-594 |
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| creator | Fazlollahi, Farnoosh, MS Angelow, Susanne, PhD Yacobi, Nazanin R., PhD Marchelletta, Ronald, PhD Yu, Alan S.L., PhD Hamm-Alvarez, Sarah F., PhD Borok, Zea, MD Kim, Kwang-Jin, PhD Crandall, Edward D., PhD, MD |
| description | Abstract Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN3 ) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4°C and after treatment with NaN3 decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type. From the Clinical Editor This basic science study investigates the membrane translocation of polystyrene nanoparticles (PNP) via an epithelial cell monolayer utilizing canine kidney cells. The authors conclude that translocation occurs via clathrin-mediated endocytosis and is dependent on the physicochemical properties of the particular PNP. |
| doi_str_mv | 10.1016/j.nano.2011.01.008 |
| format | Article |
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To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN3 ) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4°C and after treatment with NaN3 decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type. From the Clinical Editor This basic science study investigates the membrane translocation of polystyrene nanoparticles (PNP) via an epithelial cell monolayer utilizing canine kidney cells. The authors conclude that translocation occurs via clathrin-mediated endocytosis and is dependent on the physicochemical properties of the particular PNP.</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2011.01.008</identifier><identifier>PMID: 21310266</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Line ; Cell Membrane Permeability - drug effects ; Clathrin ; Clathrin - chemistry ; Clathrin - metabolism ; Dogs ; Dynamin ; Dynamins - metabolism ; Egtazic Acid - pharmacology ; Endocytosis ; Endocytosis - drug effects ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Epithelial transport ; Internal Medicine ; Nanoparticles - chemistry ; Polystyrenes - chemistry ; Polystyrenes - metabolism ; Rats ; Sodium Azide - pharmacology ; Surface charge ; Surface Properties</subject><ispartof>Nanomedicine, 2011-10, Vol.7 (5), p.588-594</ispartof><rights>Elsevier Inc.</rights><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-6581b37bbaa5e66bef53e1c3fd25fae6849b5c26e1fc31c96944359abb7517c3</citedby><cites>FETCH-LOGICAL-c541t-6581b37bbaa5e66bef53e1c3fd25fae6849b5c26e1fc31c96944359abb7517c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1549963411000128$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21310266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fazlollahi, Farnoosh, MS</creatorcontrib><creatorcontrib>Angelow, Susanne, PhD</creatorcontrib><creatorcontrib>Yacobi, Nazanin R., PhD</creatorcontrib><creatorcontrib>Marchelletta, Ronald, PhD</creatorcontrib><creatorcontrib>Yu, Alan S.L., PhD</creatorcontrib><creatorcontrib>Hamm-Alvarez, Sarah F., PhD</creatorcontrib><creatorcontrib>Borok, Zea, MD</creatorcontrib><creatorcontrib>Kim, Kwang-Jin, PhD</creatorcontrib><creatorcontrib>Crandall, Edward D., PhD, MD</creatorcontrib><title>Polystyrene nanoparticle trafficking across MDCK-II</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Abstract Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN3 ) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4°C and after treatment with NaN3 decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type. From the Clinical Editor This basic science study investigates the membrane translocation of polystyrene nanoparticles (PNP) via an epithelial cell monolayer utilizing canine kidney cells. The authors conclude that translocation occurs via clathrin-mediated endocytosis and is dependent on the physicochemical properties of the particular PNP.</description><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Clathrin</subject><subject>Clathrin - chemistry</subject><subject>Clathrin - metabolism</subject><subject>Dogs</subject><subject>Dynamin</subject><subject>Dynamins - metabolism</subject><subject>Egtazic Acid - pharmacology</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial transport</subject><subject>Internal Medicine</subject><subject>Nanoparticles - chemistry</subject><subject>Polystyrenes - chemistry</subject><subject>Polystyrenes - metabolism</subject><subject>Rats</subject><subject>Sodium Azide - pharmacology</subject><subject>Surface charge</subject><subject>Surface Properties</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vEzEQxS1ERUvhC3BAuXHaMLNee3clVAmFfxGtQKL3kdeZLU43drA3lfLt8TYlAg6VRrIlv_c88xshXiHMEVC_Xc-98WFeAuIcckHzRJyhqtqi1VX59HiX1al4ntIaQNYA7TNxWqJEKLU-E_J7GPZp3Ef2PJvitiaOzg48G6Ppe2dvnb-ZGRtDSrOrD4uvxXL5Qpz0Zkj88uE8F9efPl4vvhSX3z4vF-8vC6sqHAutGuxk3XXGKNa6415JRiv7Val6w7qp2k7ZUjP2VqJtdVtVUrWm62qFtZXn4uIQu911G15Z9rmlgbbRbUzcUzCO_n3x7ifdhDuSKPOYmAPePATE8GvHaaSNS5aHwXgOu0RNm0V1jZCV5UF5P2fk_vgLAk2saU0THJpYE-SCJpte_93f0fIHbha8Owg4Q7pzHClZx97yykW2I62Cezz_4j-7HZx31gy3vOe0DrvoM35CSiUB_Zi2PS0bEQCwbORvrtqlkA</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Fazlollahi, Farnoosh, MS</creator><creator>Angelow, Susanne, PhD</creator><creator>Yacobi, Nazanin R., PhD</creator><creator>Marchelletta, Ronald, PhD</creator><creator>Yu, Alan S.L., PhD</creator><creator>Hamm-Alvarez, Sarah F., PhD</creator><creator>Borok, Zea, MD</creator><creator>Kim, Kwang-Jin, PhD</creator><creator>Crandall, Edward D., PhD, MD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20111001</creationdate><title>Polystyrene nanoparticle trafficking across MDCK-II</title><author>Fazlollahi, Farnoosh, MS ; Angelow, Susanne, PhD ; Yacobi, Nazanin R., PhD ; Marchelletta, Ronald, PhD ; Yu, Alan S.L., PhD ; Hamm-Alvarez, Sarah F., PhD ; Borok, Zea, MD ; Kim, Kwang-Jin, PhD ; Crandall, Edward D., PhD, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-6581b37bbaa5e66bef53e1c3fd25fae6849b5c26e1fc31c96944359abb7517c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Clathrin</topic><topic>Clathrin - chemistry</topic><topic>Clathrin - metabolism</topic><topic>Dogs</topic><topic>Dynamin</topic><topic>Dynamins - metabolism</topic><topic>Egtazic Acid - pharmacology</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial transport</topic><topic>Internal Medicine</topic><topic>Nanoparticles - chemistry</topic><topic>Polystyrenes - chemistry</topic><topic>Polystyrenes - metabolism</topic><topic>Rats</topic><topic>Sodium Azide - pharmacology</topic><topic>Surface charge</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fazlollahi, Farnoosh, MS</creatorcontrib><creatorcontrib>Angelow, Susanne, PhD</creatorcontrib><creatorcontrib>Yacobi, Nazanin R., PhD</creatorcontrib><creatorcontrib>Marchelletta, Ronald, PhD</creatorcontrib><creatorcontrib>Yu, Alan S.L., PhD</creatorcontrib><creatorcontrib>Hamm-Alvarez, Sarah F., PhD</creatorcontrib><creatorcontrib>Borok, Zea, MD</creatorcontrib><creatorcontrib>Kim, Kwang-Jin, PhD</creatorcontrib><creatorcontrib>Crandall, Edward D., PhD, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fazlollahi, Farnoosh, MS</au><au>Angelow, Susanne, PhD</au><au>Yacobi, Nazanin R., PhD</au><au>Marchelletta, Ronald, PhD</au><au>Yu, Alan S.L., PhD</au><au>Hamm-Alvarez, Sarah F., PhD</au><au>Borok, Zea, MD</au><au>Kim, Kwang-Jin, PhD</au><au>Crandall, Edward D., PhD, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polystyrene nanoparticle trafficking across MDCK-II</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2011-10-01</date><risdate>2011</risdate><volume>7</volume><issue>5</issue><spage>588</spage><epage>594</epage><pages>588-594</pages><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Abstract Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN3 ) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4°C and after treatment with NaN3 decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type. From the Clinical Editor This basic science study investigates the membrane translocation of polystyrene nanoparticles (PNP) via an epithelial cell monolayer utilizing canine kidney cells. The authors conclude that translocation occurs via clathrin-mediated endocytosis and is dependent on the physicochemical properties of the particular PNP.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21310266</pmid><doi>10.1016/j.nano.2011.01.008</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Line Cell Membrane Permeability - drug effects Clathrin Clathrin - chemistry Clathrin - metabolism Dogs Dynamin Dynamins - metabolism Egtazic Acid - pharmacology Endocytosis Endocytosis - drug effects Epithelial Cells - drug effects Epithelial Cells - metabolism Epithelial transport Internal Medicine Nanoparticles - chemistry Polystyrenes - chemistry Polystyrenes - metabolism Rats Sodium Azide - pharmacology Surface charge Surface Properties |
| title | Polystyrene nanoparticle trafficking across MDCK-II |
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