The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis
Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1,...
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Veröffentlicht in: | Genes & development 2001-02, Vol.15 (3), p.352-363 |
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description | Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1, is required for cytokinesis. The activity of NPK1 increases in the late M phase of the tobacco cell cycle. During expansion of a new cross-wall (cell plate) toward the cell cortex, NPK1 is consistently localized to the equatorial zone of the phragmoplast, the cytokinetic apparatus where the cell plate is formed. Expression of a kinase-negative mutant of NPK1 results in the generation of multinucleate cells with incomplete cell plates. Phragmoplasts can be formed, but its expansion toward the cell cortex is also blocked. Thus, our results indicate that the NPK1 MAPKKK is essential for the formation of the cell plate, especially for its lateral growth. |
doi_str_mv | 10.1101/gad.863701 |
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However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1, is required for cytokinesis. The activity of NPK1 increases in the late M phase of the tobacco cell cycle. During expansion of a new cross-wall (cell plate) toward the cell cortex, NPK1 is consistently localized to the equatorial zone of the phragmoplast, the cytokinetic apparatus where the cell plate is formed. Expression of a kinase-negative mutant of NPK1 results in the generation of multinucleate cells with incomplete cell plates. Phragmoplasts can be formed, but its expansion toward the cell cortex is also blocked. Thus, our results indicate that the NPK1 MAPKKK is essential for the formation of the cell plate, especially for its lateral growth.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.863701</identifier><identifier>PMID: 11159915</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Animals ; Cell Division - physiology ; cell plate ; Gene Expression ; MAP Kinase Kinase Kinases - genetics ; MAP Kinase Kinase Kinases - physiology ; MAPKK kinase ; Mitosis - physiology ; Nicotiana ; Nicotiana - cytology ; NPK1 protein ; Phenotype ; Phosphorylation ; Plant Proteins - genetics ; Plant Proteins - physiology ; Plants, Genetically Modified ; Plants, Toxic ; Research Paper ; Signal Transduction ; Tissue Distribution</subject><ispartof>Genes & development, 2001-02, Vol.15 (3), p.352-363</ispartof><rights>Copyright © 2001, Cold Spring Harbor Laboratory Press 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-490f06326e46ed908a466761cf9a3609d848853d39f08d756abd69aedd9277263</citedby><cites>FETCH-LOGICAL-c470t-490f06326e46ed908a466761cf9a3609d848853d39f08d756abd69aedd9277263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC312623/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC312623/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11159915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nishihama, R</creatorcontrib><creatorcontrib>Ishikawa, M</creatorcontrib><creatorcontrib>Araki, S</creatorcontrib><creatorcontrib>Soyano, T</creatorcontrib><creatorcontrib>Asada, T</creatorcontrib><creatorcontrib>Machida, Y</creatorcontrib><title>The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1, is required for cytokinesis. The activity of NPK1 increases in the late M phase of the tobacco cell cycle. During expansion of a new cross-wall (cell plate) toward the cell cortex, NPK1 is consistently localized to the equatorial zone of the phragmoplast, the cytokinetic apparatus where the cell plate is formed. Expression of a kinase-negative mutant of NPK1 results in the generation of multinucleate cells with incomplete cell plates. Phragmoplasts can be formed, but its expansion toward the cell cortex is also blocked. Thus, our results indicate that the NPK1 MAPKKK is essential for the formation of the cell plate, especially for its lateral growth.</description><subject>Animals</subject><subject>Cell Division - physiology</subject><subject>cell plate</subject><subject>Gene Expression</subject><subject>MAP Kinase Kinase Kinases - genetics</subject><subject>MAP Kinase Kinase Kinases - physiology</subject><subject>MAPKK kinase</subject><subject>Mitosis - physiology</subject><subject>Nicotiana</subject><subject>Nicotiana - cytology</subject><subject>NPK1 protein</subject><subject>Phenotype</subject><subject>Phosphorylation</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - physiology</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>Research Paper</subject><subject>Signal Transduction</subject><subject>Tissue Distribution</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctOwzAQRS0EoqWw4QOQVyyQUuw4seMFC1TxEghYlLXlxpPWkMTFdir170nVitdqNDP3zkMHoVNKxpQSejnXZlxwJgjdQ0OaZzLJMyH20ZAUkiSScTlARyG8E0I44fwQDSiluZQ0H6IwXQB-fn2kuLHRzaFNdBntSkcweOldBNviD9vqAP-CDVhjD_Ou1tF57CpcQl0nyz4FXDnf6Ghdi3t7X2ojLtfR9VYINhyjg0rXAU52cYTebm-mk_vk6eXuYXL9lJSZIDHJJKkIZymHjIORpNAZ54LTspKacSJNkRVFzgyTFSmMyLmeGS41GCNTIVLORuhqO3fZzRowJbTR61otvW20Xyunrfrbae1Czd1KMZrylPX-853fu88OQlSNDZsvdQuuC4qKgqYpk73wYissvQvBQ_W9gxK1QaR6RGqLqBef_b7qR7pjwr4AcyyOcg</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Nishihama, R</creator><creator>Ishikawa, M</creator><creator>Araki, S</creator><creator>Soyano, T</creator><creator>Asada, T</creator><creator>Machida, Y</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>5PM</scope></search><sort><creationdate>20010201</creationdate><title>The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis</title><author>Nishihama, R ; Ishikawa, M ; Araki, S ; Soyano, T ; Asada, T ; Machida, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-490f06326e46ed908a466761cf9a3609d848853d39f08d756abd69aedd9277263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Cell Division - physiology</topic><topic>cell plate</topic><topic>Gene Expression</topic><topic>MAP Kinase Kinase Kinases - genetics</topic><topic>MAP Kinase Kinase Kinases - physiology</topic><topic>MAPKK kinase</topic><topic>Mitosis - physiology</topic><topic>Nicotiana</topic><topic>Nicotiana - cytology</topic><topic>NPK1 protein</topic><topic>Phenotype</topic><topic>Phosphorylation</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - physiology</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>Research Paper</topic><topic>Signal Transduction</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishihama, R</creatorcontrib><creatorcontrib>Ishikawa, M</creatorcontrib><creatorcontrib>Araki, S</creatorcontrib><creatorcontrib>Soyano, T</creatorcontrib><creatorcontrib>Asada, T</creatorcontrib><creatorcontrib>Machida, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishihama, R</au><au>Ishikawa, M</au><au>Araki, S</au><au>Soyano, T</au><au>Asada, T</au><au>Machida, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>15</volume><issue>3</issue><spage>352</spage><epage>363</epage><pages>352-363</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><abstract>Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1, is required for cytokinesis. The activity of NPK1 increases in the late M phase of the tobacco cell cycle. During expansion of a new cross-wall (cell plate) toward the cell cortex, NPK1 is consistently localized to the equatorial zone of the phragmoplast, the cytokinetic apparatus where the cell plate is formed. Expression of a kinase-negative mutant of NPK1 results in the generation of multinucleate cells with incomplete cell plates. Phragmoplasts can be formed, but its expansion toward the cell cortex is also blocked. Thus, our results indicate that the NPK1 MAPKKK is essential for the formation of the cell plate, especially for its lateral growth.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>11159915</pmid><doi>10.1101/gad.863701</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Cell Division - physiology cell plate Gene Expression MAP Kinase Kinase Kinases - genetics MAP Kinase Kinase Kinases - physiology MAPKK kinase Mitosis - physiology Nicotiana Nicotiana - cytology NPK1 protein Phenotype Phosphorylation Plant Proteins - genetics Plant Proteins - physiology Plants, Genetically Modified Plants, Toxic Research Paper Signal Transduction Tissue Distribution |
title | The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis |
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