The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis

Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1,...

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Veröffentlicht in:Genes & development 2001-02, Vol.15 (3), p.352-363
Hauptverfasser: Nishihama, R, Ishikawa, M, Araki, S, Soyano, T, Asada, T, Machida, Y
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container_issue 3
container_start_page 352
container_title Genes & development
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creator Nishihama, R
Ishikawa, M
Araki, S
Soyano, T
Asada, T
Machida, Y
description Mitogen-activated protein kinase (MAPK) cascades play important roles not only in the transduction of extracellular signals but in the progression of the cell cycle. However, evidence for their role in cytokinesis is limited. Here, we show that a tobacco MAPK kinase kinase (MAPKKK), designated NPK1, is required for cytokinesis. The activity of NPK1 increases in the late M phase of the tobacco cell cycle. During expansion of a new cross-wall (cell plate) toward the cell cortex, NPK1 is consistently localized to the equatorial zone of the phragmoplast, the cytokinetic apparatus where the cell plate is formed. Expression of a kinase-negative mutant of NPK1 results in the generation of multinucleate cells with incomplete cell plates. Phragmoplasts can be formed, but its expansion toward the cell cortex is also blocked. Thus, our results indicate that the NPK1 MAPKKK is essential for the formation of the cell plate, especially for its lateral growth.
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subjects Animals
Cell Division - physiology
cell plate
Gene Expression
MAP Kinase Kinase Kinases - genetics
MAP Kinase Kinase Kinases - physiology
MAPKK kinase
Mitosis - physiology
Nicotiana
Nicotiana - cytology
NPK1 protein
Phenotype
Phosphorylation
Plant Proteins - genetics
Plant Proteins - physiology
Plants, Genetically Modified
Plants, Toxic
Research Paper
Signal Transduction
Tissue Distribution
title The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis
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