Placental Perivascular Cells for Human Muscle Regeneration
Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation...
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| Veröffentlicht in: | Stem cells and development 2011-03, Vol.20 (3), p.451-463 |
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| creator | Park, Tea Soon Gavina, Manuela Chen, Chien-Wen Sun, Bin Teng, Pang-Ning Huard, Johnny Deasy, Bridget M. Zimmerlin, Ludovic Péault, Bruno |
| description | Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation human placenta were first transplanted intact into the gastrocnemius muscles of SCID/
mdx
mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34−CD45−CD56− cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34−CD45−CD56− perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/
mdx
mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers. |
| doi_str_mv | 10.1089/scd.2010.0354 |
| format | Article |
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mdx
mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34−CD45−CD56− cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34−CD45−CD56− perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/
mdx
mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers.</description><identifier>ISSN: 1547-3287</identifier><identifier>EISSN: 1557-8534</identifier><identifier>DOI: 10.1089/scd.2010.0354</identifier><identifier>PMID: 20923371</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Antigens, Nuclear - metabolism ; Cell Adhesion ; Cell Differentiation ; Cell Movement ; Cell Shape ; Cells, Cultured ; Chorionic Villi - metabolism ; Chorionic Villi - transplantation ; Dystrophin ; Dystrophin - metabolism ; Female ; Humans ; Mesenchymal Stromal Cells - metabolism ; Mice ; Mice, SCID ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - metabolism ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - cytology ; Muscle, Skeletal - physiology ; Neovascularization, Physiologic ; Original Research Reports ; Physiological aspects ; Placenta ; Placenta - blood supply ; Placenta - cytology ; Pregnancy ; Regeneration ; Spectrin - metabolism ; Stem cells ; Tissue Culture Techniques ; Transcription, Genetic</subject><ispartof>Stem cells and development, 2011-03, Vol.20 (3), p.451-463</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><rights>Copyright 2011, Mary Ann Liebert, Inc. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-a054c43bd0bd47b04cba5a8c63774d45b319102aeb3671b3da38e03a8fa84793</citedby><cites>FETCH-LOGICAL-c497t-a054c43bd0bd47b04cba5a8c63774d45b319102aeb3671b3da38e03a8fa84793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20923371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Tea Soon</creatorcontrib><creatorcontrib>Gavina, Manuela</creatorcontrib><creatorcontrib>Chen, Chien-Wen</creatorcontrib><creatorcontrib>Sun, Bin</creatorcontrib><creatorcontrib>Teng, Pang-Ning</creatorcontrib><creatorcontrib>Huard, Johnny</creatorcontrib><creatorcontrib>Deasy, Bridget M.</creatorcontrib><creatorcontrib>Zimmerlin, Ludovic</creatorcontrib><creatorcontrib>Péault, Bruno</creatorcontrib><title>Placental Perivascular Cells for Human Muscle Regeneration</title><title>Stem cells and development</title><addtitle>Stem Cells Dev</addtitle><description>Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation human placenta were first transplanted intact into the gastrocnemius muscles of SCID/
mdx
mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34−CD45−CD56− cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34−CD45−CD56− perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/
mdx
mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers.</description><subject>Animals</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, Nuclear - metabolism</subject><subject>Cell Adhesion</subject><subject>Cell Differentiation</subject><subject>Cell Movement</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Chorionic Villi - metabolism</subject><subject>Chorionic Villi - transplantation</subject><subject>Dystrophin</subject><subject>Dystrophin - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Muscle Fibers, Skeletal - cytology</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Neovascularization, Physiologic</subject><subject>Original Research Reports</subject><subject>Physiological aspects</subject><subject>Placenta</subject><subject>Placenta - blood supply</subject><subject>Placenta - cytology</subject><subject>Pregnancy</subject><subject>Regeneration</subject><subject>Spectrin - metabolism</subject><subject>Stem cells</subject><subject>Tissue Culture Techniques</subject><subject>Transcription, Genetic</subject><issn>1547-3287</issn><issn>1557-8534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFTEUxYNYbK0u3cqAC1fzzNdMMi6E8rBWaGkp3Yc7mTvPSCapyUyh_72ZvlosCJJFcm9-93APh5B3jG4Y1d2nbIcNp6WiopEvyBFrGlXrRsiX61uqWnCtDsnrnH9Syluu5StyyGnHhVDsiHy-8mAxzOCrK0zuDrJdPKRqi97naoypOlsmCNXFkq3H6hp3GDDB7GJ4Qw5G8BnfPt7H5Ob06832rD6__PZ9e3JeW9mpuQbaSCtFP9B-kKqn0vbQgLatUEoOsukF6xjlgL1oFevFAEIjFaBH0FJ14ph82cveLv2Ew7psAm9uk5sg3ZsIzjz_Ce6H2cU7I1ix-SDw8VEgxV8L5tlMLtviDwLGJRvdaiqY4KqQH_bkDjwaF8ZYBO1KmxPeFDXKWlqozT-ocgacnI0BR1f6zwbq_YBNMeeE49PyjJo1RFNCNGuIZg2x8O__dvxE_0mtAGIPrG0IwTvsMc3_kf0NHq-n6w</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Park, Tea Soon</creator><creator>Gavina, Manuela</creator><creator>Chen, Chien-Wen</creator><creator>Sun, Bin</creator><creator>Teng, Pang-Ning</creator><creator>Huard, Johnny</creator><creator>Deasy, Bridget M.</creator><creator>Zimmerlin, Ludovic</creator><creator>Péault, Bruno</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>Placental Perivascular Cells for Human Muscle Regeneration</title><author>Park, Tea Soon ; Gavina, Manuela ; Chen, Chien-Wen ; Sun, Bin ; Teng, Pang-Ning ; Huard, Johnny ; Deasy, Bridget M. ; Zimmerlin, Ludovic ; Péault, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-a054c43bd0bd47b04cba5a8c63774d45b319102aeb3671b3da38e03a8fa84793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, Nuclear - metabolism</topic><topic>Cell Adhesion</topic><topic>Cell Differentiation</topic><topic>Cell Movement</topic><topic>Cell Shape</topic><topic>Cells, Cultured</topic><topic>Chorionic Villi - metabolism</topic><topic>Chorionic Villi - transplantation</topic><topic>Dystrophin</topic><topic>Dystrophin - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mice</topic><topic>Mice, SCID</topic><topic>Muscle Fibers, Skeletal - cytology</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Neovascularization, Physiologic</topic><topic>Original Research Reports</topic><topic>Physiological aspects</topic><topic>Placenta</topic><topic>Placenta - blood supply</topic><topic>Placenta - cytology</topic><topic>Pregnancy</topic><topic>Regeneration</topic><topic>Spectrin - metabolism</topic><topic>Stem cells</topic><topic>Tissue Culture Techniques</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Tea Soon</creatorcontrib><creatorcontrib>Gavina, Manuela</creatorcontrib><creatorcontrib>Chen, Chien-Wen</creatorcontrib><creatorcontrib>Sun, Bin</creatorcontrib><creatorcontrib>Teng, Pang-Ning</creatorcontrib><creatorcontrib>Huard, Johnny</creatorcontrib><creatorcontrib>Deasy, Bridget M.</creatorcontrib><creatorcontrib>Zimmerlin, Ludovic</creatorcontrib><creatorcontrib>Péault, Bruno</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Tea Soon</au><au>Gavina, Manuela</au><au>Chen, Chien-Wen</au><au>Sun, Bin</au><au>Teng, Pang-Ning</au><au>Huard, Johnny</au><au>Deasy, Bridget M.</au><au>Zimmerlin, Ludovic</au><au>Péault, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Placental Perivascular Cells for Human Muscle Regeneration</atitle><jtitle>Stem cells and development</jtitle><addtitle>Stem Cells Dev</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>20</volume><issue>3</issue><spage>451</spage><epage>463</epage><pages>451-463</pages><issn>1547-3287</issn><eissn>1557-8534</eissn><abstract>Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation human placenta were first transplanted intact into the gastrocnemius muscles of SCID/
mdx
mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34−CD45−CD56− cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34−CD45−CD56− perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/
mdx
mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>20923371</pmid><doi>10.1089/scd.2010.0354</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Stem cells and development, 2011-03, Vol.20 (3), p.451-463 |
| issn | 1547-3287 1557-8534 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigens, CD - genetics Antigens, CD - metabolism Antigens, Nuclear - metabolism Cell Adhesion Cell Differentiation Cell Movement Cell Shape Cells, Cultured Chorionic Villi - metabolism Chorionic Villi - transplantation Dystrophin Dystrophin - metabolism Female Humans Mesenchymal Stromal Cells - metabolism Mice Mice, SCID Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - metabolism Muscle, Skeletal - blood supply Muscle, Skeletal - cytology Muscle, Skeletal - physiology Neovascularization, Physiologic Original Research Reports Physiological aspects Placenta Placenta - blood supply Placenta - cytology Pregnancy Regeneration Spectrin - metabolism Stem cells Tissue Culture Techniques Transcription, Genetic |
| title | Placental Perivascular Cells for Human Muscle Regeneration |
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