Cationic porphyrins as probes of DNA structure

Cationic porphyrins as probes of DNA structure

The DNA binding specificity of a group of cationic manganese porphyrin complexes has been examined using DNase I footprinting methodology and by observing the sites of porphyrin-induced DNA strand scission in the presence of potassium superoxide. The compounds, which possess systematic changes in to...

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Veröffentlicht in:Nucleic acids research 1986-11, Vol.14 (22), p.9133-9148
Hauptverfasser: Bromley, Susan D., Ward, Brian W., Dabrowiak, James C.
Format: Artikel
Sprache:eng
Schlagworte:
Applied sciences
Base Sequence
Chemical Phenomena
Chemistry
Deoxyribonuclease I
DNA
Exact sciences and technology
Other techniques and industries
Porphyrins
Structure-Activity Relationship
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container_end_page 9148
container_issue 22
container_start_page 9133
container_title Nucleic acids research
container_volume 14
creator Bromley, Susan D.
Ward, Brian W.
Dabrowiak, James C.
description The DNA binding specificity of a group of cationic manganese porphyrin complexes has been examined using DNase I footprinting methodology and by observing the sites of porphyrin-induced DNA strand scission in the presence of potassium superoxide. The compounds, which possess systematic changes in total charge, its distribution on the periphery on the macrocycle and ligand shape, bind in the minor groove of AT rich regions of DNA. While changes in total charge and charge arrangement do not significantly influence specificity, a shape change which blocks close ligand contact with the minor groove relaxes the original AT specificity causing the compound to cleave at both AT and GC sites. The observed changes in binding sequence specificity were interpreted in terms of electrostatic and steric factors associated with both the compounds and DNA.
doi_str_mv 10.1093/nar/14.22.9133
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source Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central
subjects Applied sciences
Base Sequence
Chemical Phenomena
Chemistry
Deoxyribonuclease I
DNA
Exact sciences and technology
Other techniques and industries
Porphyrins
Structure-Activity Relationship
title Cationic porphyrins as probes of DNA structure
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