Rapid Detection of Urinary Tract Infections Using Isotachophoresis and Molecular Beacons

We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria sp...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-06, Vol.83 (11), p.4110-4117
Hauptverfasser:	Bercovici, M, Kaigala, G. V, Mach, K. E, Han, C. M, Liao, J. C, Santiago, J. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bacteria - genetics Bacteria - isolation & purification Chemical compounds Chemical reactions Chemistry Chromatographic methods and physical methods associated with chromatography E coli Escherichia coli - genetics Exact sciences and technology Fluorescent Dyes - chemistry Humans Isotachophoresis - methods Microfluidic Analytical Techniques - methods Molecular structure Other chromatographic methods Ribonucleic acid RNA RNA, Ribosomal, 16S - analysis Urinary tract diseases Urinary Tract Infections - diagnosis Urinary Tract Infections - microbiology
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