Rapid Detection of Urinary Tract Infections Using Isotachophoresis and Molecular Beacons
We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria sp...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2011-06, Vol.83 (11), p.4110-4117 |
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| creator | Bercovici, M Kaigala, G. V Mach, K. E Han, C. M Liao, J. C Santiago, J. G |
| description | We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used molecular beacons to achieve detection of bacteria specific sequences. We demonstrated detection of E. coli in bacteria cultures as well as in patient urine samples in the clinically relevant range 1E6–1E8 cfu/mL. For bacterial cultures we further demonstrate quantification in this range. The assay requires minimal sample preparation (a single centrifugation and dilution), and can be completed, from beginning of lysing to detection, in under 15 min. We believe that the principles presented here can be used for design of other rapid diagnostics or detection methods for pathogenic diseases. |
| doi_str_mv | 10.1021/ac200253x |
| format | Article |
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We demonstrated detection of E. coli in bacteria cultures as well as in patient urine samples in the clinically relevant range 1E6–1E8 cfu/mL. For bacterial cultures we further demonstrate quantification in this range. The assay requires minimal sample preparation (a single centrifugation and dilution), and can be completed, from beginning of lysing to detection, in under 15 min. We believe that the principles presented here can be used for design of other rapid diagnostics or detection methods for pathogenic diseases.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21545089</pmid><doi>10.1021/ac200253x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2011-06, Vol.83 (11), p.4110-4117 |
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| subjects | Analytical chemistry Bacteria - genetics Bacteria - isolation & purification Chemical compounds Chemical reactions Chemistry Chromatographic methods and physical methods associated with chromatography E coli Escherichia coli - genetics Exact sciences and technology Fluorescent Dyes - chemistry Humans Isotachophoresis - methods Microfluidic Analytical Techniques - methods Molecular structure Other chromatographic methods Ribonucleic acid RNA RNA, Ribosomal, 16S - analysis Urinary tract diseases Urinary Tract Infections - diagnosis Urinary Tract Infections - microbiology |
| title | Rapid Detection of Urinary Tract Infections Using Isotachophoresis and Molecular Beacons |
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