Visualization of Oligonucleotide Probes and Point Mutations in Interphase Nuclei and DNA Fibers Using Rolling Circle DNA Amplification

Rolling circle amplification (RCA) is a surface-anchored DNA replication reaction that can be exploited to visualize single molecular recognition events. Here we report the use of RCA to visualize target DNA sequences as small as 50 nts in peripheral blood lymphocytes or in stretched DNA fibers. Thr...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2001-03, Vol.98 (7), p.3940-3945
Hauptverfasser:	Zhong, Xiao-bo, Lizardi, Paul M., Huang, Xiao-hua, Bray-Ward, Patricia L., Ward, David C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Biological Sciences BRCA-1 gene BRCA1 gene Cell Line Cell lines Cell Nucleus - genetics Cystic Fibrosis Transmembrane Conductance Regulator - genetics Deoxyribonucleic acid DNA DNA - analysis DNA Mutational Analysis - methods DNA probes Genes Genetic loci Genetic mutation Genetics Gorlin syndrome HeLa cells Humans Interphase Interphase - genetics Ligation Mutation Nucleic Acid Amplification Techniques Oligonucleotide Probes Point Mutation Polysorbates rolling circle amplification
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