Fluoride-Cleavable, Fluorescently Labelled Reversible Terminators: Synthesis and Use in Primer Extension

Fluorescent 2′‐deoxynucleotides containing a protecting group at the 3′‐O‐position are reversible terminators that enable array‐based DNA sequencing‐by‐synthesis (SBS) approaches. Herein, we describe the synthesis and full characterisation of four reversible terminators bearing a 3′‐blocking moiety...

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Veröffentlicht in:	Chemistry : a European journal 2011-03, Vol.17 (10), p.2903-2915
Hauptverfasser:	Knapp, Diana C., Serva, Saulius, D'Onofrio, Jennifer, Keller, Angelika, Lubys, Arvydas, Kurg, Ants, Remm, Maido, Engels, Joachim W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical compounds Chemistry Deoxyribonucleic acid DNA DNA polymerase DNA Primers - metabolism Fluorescence Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry fluoride ions Fluorides Fluorides - chemistry Leukemia Virus, Murine - enzymology Molecular Structure Nucleotides Oligonucleotide Probes - chemical synthesis Oligonucleotide Probes - chemistry Oligonucleotides reverse transcriptase reversible terminators RNA-Directed DNA Polymerase - metabolism Stopping Synthesis
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