Comprehensive analysis of the chromatin landscape in Drosophila
Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which impact cell differentiation, gene regulation and other key cellular processes. We present a genome-wide chromatin landscape for Drosophila melanogaster based on 18 histone modifications, summarized by 9 p...
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| Veröffentlicht in: | Nature (London) 2010-12, Vol.471 (7339), p.480-485 |
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| creator | Kharchenko, Peter V. Alekseyenko, Artyom A. Schwartz, Yuri B. Minoda, Aki Riddle, Nicole C. Ernst, Jason Sabo, Peter J. Larschan, Erica Gorchakov, Andrey A. Gu, Tingting Linder-Basso, Daniela Plachetka, Annette Shanower, Gregory Tolstorukov, Michael Y. Luquette, Lovelace J. Xi, Ruibin Jung, Youngsook L. Park, Richard W. Bishop, Eric P. Canfield, Theresa P. Sandstrom, Richard Thurman, Robert E. MacAlpine, David M. Stamatoyannopoulos, John A. Kellis, Manolis Elgin, Sarah C. R. Kuroda, Mitzi I. Pirrotta, Vincenzo Karpen, Gary H. Park, Peter J. |
| description | Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which impact cell differentiation, gene regulation and other key cellular processes. We present a genome-wide chromatin landscape for
Drosophila melanogaster
based on 18 histone modifications, summarized by 9 prevalent combinatorial patterns. Integrative analysis with other data (non-histone chromatin proteins, DNaseI hypersensitivity, GRO-seq reads produced by engaged polymerase, short/long RNA products) reveals discrete characteristics of chromosomes, genes, regulatory elements, and other functional domains. We find that active genes display distinct chromatin signatures that are correlated with disparate gene lengths, exon patterns, regulatory functions, and genomic contexts. We also demonstrate a diversity of signatures among Polycomb targets that include a subset with paused polymerase. This systematic profiling and integrative analysis of chromatin signatures provides insights into how genomic elements are regulated, and will serve as a resource for future experimental investigations of genome structure and function. |
| doi_str_mv | 10.1038/nature09725 |
| format | Article |
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Drosophila melanogaster
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| title | Comprehensive analysis of the chromatin landscape in Drosophila |
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