Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT
To characterize postnatal changes in eye size in glaucomatous DBA/2J (D2) mice and in nonglaucomatous C57BL/6J mice (B6) in vivo by means of whole-eye optical coherence tomography (OCT). D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-doma...
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| creator | Chou, Tsung-Han Kocaoglu, Omer P Borja, David Ruggeri, Marco Uhlhorn, Stephen R Manns, Fabrice Porciatti, Vittorio |
| description | To characterize postnatal changes in eye size in glaucomatous DBA/2J (D2) mice and in nonglaucomatous C57BL/6J mice (B6) in vivo by means of whole-eye optical coherence tomography (OCT).
D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-domain OCT system with a center wavelength of 825 nm and an axial scan length of 7.1 mm produced axial A-scan interferograms at a rate of 20 A-lines/s with a resolution of 8 μm. Axial length (AL), corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and retinal thickness (RT) were measured in the optical axis and adjusted with corresponding refractive indices. Corneal curvature (CC) and IOP were also measured.
AL increased (P < 0.001) more in the D2 (21%) than in the B6 (9%) mice. There was an interaction effect (two-way ANOVA, P < 0.001) between age and strain for AL, CT, ACD, and VCD. In the D2 mice, the lens became dislocated posteriorly. Multiple regression analysis in the D2 mice revealed an independent effect of age and IOP (P ≤ 0.01) on axial length. CC steepened in the older D2 mice, whereas it flattened in the B6 mice.
In D2 mice, postnatal elongation of AL is larger than that in B6 mice and is associated with a greater increase in ACD and IOP, which seems to be a causal factor. The ease of use, short acquisition time, and noninvasiveness of whole-eye OCT make it suitable for routine use in longitudinal studies of mouse models. |
| doi_str_mv | 10.1167/iovs.10-6340 |
| format | Article |
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D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-domain OCT system with a center wavelength of 825 nm and an axial scan length of 7.1 mm produced axial A-scan interferograms at a rate of 20 A-lines/s with a resolution of 8 μm. Axial length (AL), corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and retinal thickness (RT) were measured in the optical axis and adjusted with corresponding refractive indices. Corneal curvature (CC) and IOP were also measured.
AL increased (P < 0.001) more in the D2 (21%) than in the B6 (9%) mice. There was an interaction effect (two-way ANOVA, P < 0.001) between age and strain for AL, CT, ACD, and VCD. In the D2 mice, the lens became dislocated posteriorly. Multiple regression analysis in the D2 mice revealed an independent effect of age and IOP (P ≤ 0.01) on axial length. CC steepened in the older D2 mice, whereas it flattened in the B6 mice.
In D2 mice, postnatal elongation of AL is larger than that in B6 mice and is associated with a greater increase in ACD and IOP, which seems to be a causal factor. The ease of use, short acquisition time, and noninvasiveness of whole-eye OCT make it suitable for routine use in longitudinal studies of mouse models.</description><identifier>ISSN: 1552-5783</identifier><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.10-6340</identifier><identifier>PMID: 21372015</identifier><language>eng</language><publisher>United States: Association for Research in Vision and Ophthalmology, Inc</publisher><subject>Aging - physiology ; Animals ; Anterior Chamber - pathology ; Axial Length, Eye - pathology ; Biometry ; Body Weights and Measures ; Cornea - pathology ; Glaucoma - diagnosis ; Intraocular Pressure ; Lens, Crystalline - pathology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Retina - pathology ; Tomography, Optical Coherence ; Tonometry, Ocular ; Vitreous Body - pathology</subject><ispartof>Investigative ophthalmology & visual science, 2011-05, Vol.52 (6), p.3604-3612</ispartof><rights>Copyright © Association for Research in Vision and Ophthalmology 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-37b3afa2db7718c91f00d01217190c2846b9762442ee8a1a8cd652f2721b3f843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109044/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109044/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21372015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chou, Tsung-Han</creatorcontrib><creatorcontrib>Kocaoglu, Omer P</creatorcontrib><creatorcontrib>Borja, David</creatorcontrib><creatorcontrib>Ruggeri, Marco</creatorcontrib><creatorcontrib>Uhlhorn, Stephen R</creatorcontrib><creatorcontrib>Manns, Fabrice</creatorcontrib><creatorcontrib>Porciatti, Vittorio</creatorcontrib><title>Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To characterize postnatal changes in eye size in glaucomatous DBA/2J (D2) mice and in nonglaucomatous C57BL/6J mice (B6) in vivo by means of whole-eye optical coherence tomography (OCT).
D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-domain OCT system with a center wavelength of 825 nm and an axial scan length of 7.1 mm produced axial A-scan interferograms at a rate of 20 A-lines/s with a resolution of 8 μm. Axial length (AL), corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and retinal thickness (RT) were measured in the optical axis and adjusted with corresponding refractive indices. Corneal curvature (CC) and IOP were also measured.
AL increased (P < 0.001) more in the D2 (21%) than in the B6 (9%) mice. There was an interaction effect (two-way ANOVA, P < 0.001) between age and strain for AL, CT, ACD, and VCD. In the D2 mice, the lens became dislocated posteriorly. Multiple regression analysis in the D2 mice revealed an independent effect of age and IOP (P ≤ 0.01) on axial length. CC steepened in the older D2 mice, whereas it flattened in the B6 mice.
In D2 mice, postnatal elongation of AL is larger than that in B6 mice and is associated with a greater increase in ACD and IOP, which seems to be a causal factor. The ease of use, short acquisition time, and noninvasiveness of whole-eye OCT make it suitable for routine use in longitudinal studies of mouse models.</description><subject>Aging - physiology</subject><subject>Animals</subject><subject>Anterior Chamber - pathology</subject><subject>Axial Length, Eye - pathology</subject><subject>Biometry</subject><subject>Body Weights and Measures</subject><subject>Cornea - pathology</subject><subject>Glaucoma - diagnosis</subject><subject>Intraocular Pressure</subject><subject>Lens, Crystalline - pathology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred DBA</subject><subject>Retina - pathology</subject><subject>Tomography, Optical Coherence</subject><subject>Tonometry, Ocular</subject><subject>Vitreous Body - pathology</subject><issn>1552-5783</issn><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc9PwjAUxxujEURvnk1vXhz0tdu6eTAB_B0SPOC56boOarZ1rgOCf70sIMHTey_vk-97yQehayB9gJAPjF25PhAvZD45QV0IAuoFPGKnR30HXTj3RQgFoOQcdSgwTgkEXVR9WNeUspE51rkt57IxtsQ2w3qjsTM_GpsSP46GA_qOC6M0VraoZK1TvDbNAo8DPpoMwt3uvmVXZmWxLGW-ccbtoPXC5tprA6fj2SU6y2Tu9NW-9tDn89Ns_OpNpi9v4-HEUyxijcd4wmQmaZpwDpGKISMkJUCBQ0wUjfwwiXlIfZ9qHUmQkUrDgGaUU0hYFvmshx52udUyKXSqdNnUMhdVbQpZb4SVRvzflGYh5nYlGJCY-G3A7T6gtt9L7RpRGKd0nstS26UTESc09oHGW_JuR6raOlfr7HAFiGgdidZRO7SOtvjN8WcH-E8K-wV2Box3</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Chou, Tsung-Han</creator><creator>Kocaoglu, Omer P</creator><creator>Borja, David</creator><creator>Ruggeri, Marco</creator><creator>Uhlhorn, Stephen R</creator><creator>Manns, Fabrice</creator><creator>Porciatti, Vittorio</creator><general>Association for Research in Vision and Ophthalmology, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110501</creationdate><title>Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT</title><author>Chou, Tsung-Han ; Kocaoglu, Omer P ; Borja, David ; Ruggeri, Marco ; Uhlhorn, Stephen R ; Manns, Fabrice ; Porciatti, Vittorio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-37b3afa2db7718c91f00d01217190c2846b9762442ee8a1a8cd652f2721b3f843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aging - physiology</topic><topic>Animals</topic><topic>Anterior Chamber - pathology</topic><topic>Axial Length, Eye - pathology</topic><topic>Biometry</topic><topic>Body Weights and Measures</topic><topic>Cornea - pathology</topic><topic>Glaucoma - diagnosis</topic><topic>Intraocular Pressure</topic><topic>Lens, Crystalline - pathology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred DBA</topic><topic>Retina - pathology</topic><topic>Tomography, Optical Coherence</topic><topic>Tonometry, Ocular</topic><topic>Vitreous Body - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chou, Tsung-Han</creatorcontrib><creatorcontrib>Kocaoglu, Omer P</creatorcontrib><creatorcontrib>Borja, David</creatorcontrib><creatorcontrib>Ruggeri, Marco</creatorcontrib><creatorcontrib>Uhlhorn, Stephen R</creatorcontrib><creatorcontrib>Manns, Fabrice</creatorcontrib><creatorcontrib>Porciatti, Vittorio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chou, Tsung-Han</au><au>Kocaoglu, Omer P</au><au>Borja, David</au><au>Ruggeri, Marco</au><au>Uhlhorn, Stephen R</au><au>Manns, Fabrice</au><au>Porciatti, Vittorio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>52</volume><issue>6</issue><spage>3604</spage><epage>3612</epage><pages>3604-3612</pages><issn>1552-5783</issn><issn>0146-0404</issn><eissn>1552-5783</eissn><abstract>To characterize postnatal changes in eye size in glaucomatous DBA/2J (D2) mice and in nonglaucomatous C57BL/6J mice (B6) in vivo by means of whole-eye optical coherence tomography (OCT).
D2 (n = 32) and B6 (n = 36) mice were tested between 2 and 20 months of age in eight age bins. A custom time-domain OCT system with a center wavelength of 825 nm and an axial scan length of 7.1 mm produced axial A-scan interferograms at a rate of 20 A-lines/s with a resolution of 8 μm. Axial length (AL), corneal thickness (CT), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), and retinal thickness (RT) were measured in the optical axis and adjusted with corresponding refractive indices. Corneal curvature (CC) and IOP were also measured.
AL increased (P < 0.001) more in the D2 (21%) than in the B6 (9%) mice. There was an interaction effect (two-way ANOVA, P < 0.001) between age and strain for AL, CT, ACD, and VCD. In the D2 mice, the lens became dislocated posteriorly. Multiple regression analysis in the D2 mice revealed an independent effect of age and IOP (P ≤ 0.01) on axial length. CC steepened in the older D2 mice, whereas it flattened in the B6 mice.
In D2 mice, postnatal elongation of AL is larger than that in B6 mice and is associated with a greater increase in ACD and IOP, which seems to be a causal factor. The ease of use, short acquisition time, and noninvasiveness of whole-eye OCT make it suitable for routine use in longitudinal studies of mouse models.</abstract><cop>United States</cop><pub>Association for Research in Vision and Ophthalmology, Inc</pub><pmid>21372015</pmid><doi>10.1167/iovs.10-6340</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Aging - physiology Animals Anterior Chamber - pathology Axial Length, Eye - pathology Biometry Body Weights and Measures Cornea - pathology Glaucoma - diagnosis Intraocular Pressure Lens, Crystalline - pathology Mice Mice, Inbred C57BL Mice, Inbred DBA Retina - pathology Tomography, Optical Coherence Tonometry, Ocular Vitreous Body - pathology |
| title | Postnatal elongation of eye size in DBA/2J mice compared with C57BL/6J mice: in vivo analysis with whole-eye OCT |
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