Sequencing multimegabase-template DNA with BigDye terminator chemistry

Using the recently introduced BigDye terminators, large-template DNA can be directly sequenced with custom primers on automated instruments. Cycle sequencing conditions are presented to sequence DNA samples isolated from a number of microbial genomes including 750-kb Ureaplasma urealyticum, 1.2-Mb M...

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Veröffentlicht in:	Genome research 1998-05, Vol.8 (5), p.557-561
Hauptverfasser:	Heiner, C R, Hunkapiller, K L, Chen, S M, Glass, J I, Chen, E Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA Primers DNA, Bacterial - analysis DNA, Bacterial - chemistry Escherichia coli - genetics Fluorescent Dyes Genome Methods Genome, Bacterial Molecular Sequence Data Mycoplasma fermentans - genetics Reproducibility of Results Sequence Analysis, DNA - instrumentation Sequence Analysis, DNA - methods Streptococcus pneumoniae - genetics Templates, Genetic
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