Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis
We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that pres...
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| Veröffentlicht in: | Genome research 1997-11, Vol.7 (11), p.1085-1093 |
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| container_title | Genome research |
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| creator | Brady, K P Rowe, L B Her, H Stevens, T J Eppig, J Sussman, D J Sikela, J Beier, D R |
| description | We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism. |
| doi_str_mv | 10.1101/gr.7.11.1085 |
| format | Article |
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In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. 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In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.</description><subject>Animals</subject><subject>Chromosome Mapping</subject><subject>Crosses, Genetic</subject><subject>DNA Mutational Analysis - methods</subject><subject>Gene Library</subject><subject>Genetic Markers</subject><subject>Letters</subject><subject>Mice</subject><subject>Mice, Inbred AKR - genetics</subject><subject>Mice, Inbred C3H - genetics</subject><subject>Mice, Inbred C57BL - genetics</subject><subject>Mice, Inbred DBA - genetics</subject><subject>Mice, Inbred Strains - genetics</subject><subject>Muridae - genetics</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Sequence Tagged Sites</subject><subject>Transcription, Genetic</subject><issn>1088-9051</issn><issn>1549-5469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1TAQjBCo9IMbVySfOJGHN3Zi58ABVVCQKnGhZ8tx1q9GsR3spOL9EP5vnfapglMv9qxndjTeraq3QHcAFD7u004UtAMq2xfVKbS8r1ve9S8LplLWPW3hdXWW8y9KKeNSnlQnPRMgOD-t_l5hwMUZ4vU8u7An0ZKma8gUjSMjJneHI7EpeoJ_5oQ5lzLj7xWDwUxcIJr4NbmABS-Y8ozG2WJnUsyZrHmz3I4J67wkHUZiYrAxeb24GPRE5jgdfEzzrcue6PJyyC5fVK-snjK-Od7n1c3XLz8vv9XXP66-X36-rg0X7VI3w2j1wJAC181oeKtFy4DZTo6jRgkCWGM0HyQKawvBB-AGWGFZA52l7Lz69Og7r4PH0WAoGSc1J-d1OqionfqfCe5W7eOdYkA72Zb-98f-FMtM8qK8ywanSQeMa1ai542QDTwrhI6B6GFL9OFR-DDAhPYpDFC1rVvtkxIFqW3dRf7u3w88iY_7Zfeyb6sj</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Brady, K P</creator><creator>Rowe, L B</creator><creator>Her, H</creator><creator>Stevens, T J</creator><creator>Eppig, J</creator><creator>Sussman, D J</creator><creator>Sikela, J</creator><creator>Beier, D R</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19971101</creationdate><title>Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis</title><author>Brady, K P ; Rowe, L B ; Her, H ; Stevens, T J ; Eppig, J ; Sussman, D J ; Sikela, J ; Beier, D R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-2bdfab3e014a2dc45a75313f68ddae817132ca4b8e7ff3134b14c138dd3216f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Chromosome Mapping</topic><topic>Crosses, Genetic</topic><topic>DNA Mutational Analysis - methods</topic><topic>Gene Library</topic><topic>Genetic Markers</topic><topic>Letters</topic><topic>Mice</topic><topic>Mice, Inbred AKR - genetics</topic><topic>Mice, Inbred C3H - genetics</topic><topic>Mice, Inbred C57BL - genetics</topic><topic>Mice, Inbred DBA - genetics</topic><topic>Mice, Inbred Strains - genetics</topic><topic>Muridae - genetics</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Sequence Tagged Sites</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brady, K P</creatorcontrib><creatorcontrib>Rowe, L B</creatorcontrib><creatorcontrib>Her, H</creatorcontrib><creatorcontrib>Stevens, T J</creatorcontrib><creatorcontrib>Eppig, J</creatorcontrib><creatorcontrib>Sussman, D J</creatorcontrib><creatorcontrib>Sikela, J</creatorcontrib><creatorcontrib>Beier, D R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brady, K P</au><au>Rowe, L B</au><au>Her, H</au><au>Stevens, T J</au><au>Eppig, J</au><au>Sussman, D J</au><au>Sikela, J</au><au>Beier, D R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis</atitle><jtitle>Genome research</jtitle><addtitle>Genome Res</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>7</volume><issue>11</issue><spage>1085</spage><epage>1093</epage><pages>1085-1093</pages><issn>1088-9051</issn><eissn>1549-5469</eissn><abstract>We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>9371744</pmid><doi>10.1101/gr.7.11.1085</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genome research, 1997-11, Vol.7 (11), p.1085-1093 |
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| language | eng |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Chromosome Mapping Crosses, Genetic DNA Mutational Analysis - methods Gene Library Genetic Markers Letters Mice Mice, Inbred AKR - genetics Mice, Inbred C3H - genetics Mice, Inbred C57BL - genetics Mice, Inbred DBA - genetics Mice, Inbred Strains - genetics Muridae - genetics Polymorphism, Single-Stranded Conformational Sequence Tagged Sites Transcription, Genetic |
| title | Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis |
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