Characterization of short tandem repeats from thirty-one human telomeres
Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR as...
Gespeichert in:
| Veröffentlicht in: | Genome research 1997-09, Vol.7 (9), p.917-923 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 923 |
|---|---|
| container_issue | 9 |
| container_start_page | 917 |
| container_title | Genome research |
| container_volume | 7 |
| creator | Rosenberg, M Hui, L Ma, J Nusbaum, H C Clark, K Robinson, L Dziadzio, L Swain, P M Keith, T Hudson, T J Biesecker, L G Flint, J |
| description | Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome. |
| doi_str_mv | 10.1101/gr.7.9.917 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_310668</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16257379</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-511aa87c0869a31e082673a4ab0fafcf845ffe265e3ad0146f3d401a2515630a3</originalsourceid><addsrcrecordid>eNqFkU1rFEEQhpugxJh4yV2Ykwdh1qrt74MHWdQEArkk56YyW70zMjO9dvcK8ddnJEvQk6cqqOctXniEuERYIQJ-2uWVXfmVR3sizlAr32pl_KtlB-daDxrfiLel_AAAqZw7FadeolLenomrTU-Zusp5-E11SHOTYlP6lGtTad7y1GTeM9XSxJympvZDro9tmrnpDxPNTeUxTZy5XIjXkcbC747zXNx_-3q3uWpvbr9fb77ctJ0CWVuNSORsB854ksjg1sZKUvQAkWIXndIx8tpolrQFVCbKrQKktUZtJJA8F5-f_-4PDxNvO55rpjHs8zBRfgyJhvDvZR76sEu_gkQwxi35D8d8Tj8PXGqYhtLxONLM6VCC9RK8lfa_IJq1Xji_gB-fwS6nUjLHlzII4Y-fsMvBBh8WPwv8_u_6L-hRiHwC8e-M7g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16257379</pqid></control><display><type>article</type><title>Characterization of short tandem repeats from thirty-one human telomeres</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Rosenberg, M ; Hui, L ; Ma, J ; Nusbaum, H C ; Clark, K ; Robinson, L ; Dziadzio, L ; Swain, P M ; Keith, T ; Hudson, T J ; Biesecker, L G ; Flint, J</creator><creatorcontrib>Rosenberg, M ; Hui, L ; Ma, J ; Nusbaum, H C ; Clark, K ; Robinson, L ; Dziadzio, L ; Swain, P M ; Keith, T ; Hudson, T J ; Biesecker, L G ; Flint, J</creatorcontrib><description>Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.</description><identifier>ISSN: 1088-9051</identifier><identifier>EISSN: 1549-5469</identifier><identifier>DOI: 10.1101/gr.7.9.917</identifier><identifier>PMID: 9314497</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Base Sequence ; Chromosome Mapping ; Chromosomes, Human - genetics ; Humans ; Letters ; Molecular Sequence Data ; Repetitive Sequences, Nucleic Acid - genetics ; Sequence Analysis, DNA ; Sequence Tagged Sites ; Telomere - genetics</subject><ispartof>Genome research, 1997-09, Vol.7 (9), p.917-923</ispartof><rights>Copyright © 1997, Cold Spring Harbor Laboratory Press 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-511aa87c0869a31e082673a4ab0fafcf845ffe265e3ad0146f3d401a2515630a3</citedby><cites>FETCH-LOGICAL-c403t-511aa87c0869a31e082673a4ab0fafcf845ffe265e3ad0146f3d401a2515630a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC310668/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC310668/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9314497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosenberg, M</creatorcontrib><creatorcontrib>Hui, L</creatorcontrib><creatorcontrib>Ma, J</creatorcontrib><creatorcontrib>Nusbaum, H C</creatorcontrib><creatorcontrib>Clark, K</creatorcontrib><creatorcontrib>Robinson, L</creatorcontrib><creatorcontrib>Dziadzio, L</creatorcontrib><creatorcontrib>Swain, P M</creatorcontrib><creatorcontrib>Keith, T</creatorcontrib><creatorcontrib>Hudson, T J</creatorcontrib><creatorcontrib>Biesecker, L G</creatorcontrib><creatorcontrib>Flint, J</creatorcontrib><title>Characterization of short tandem repeats from thirty-one human telomeres</title><title>Genome research</title><addtitle>Genome Res</addtitle><description>Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.</description><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human - genetics</subject><subject>Humans</subject><subject>Letters</subject><subject>Molecular Sequence Data</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Tagged Sites</subject><subject>Telomere - genetics</subject><issn>1088-9051</issn><issn>1549-5469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFEEQhpugxJh4yV2Ykwdh1qrt74MHWdQEArkk56YyW70zMjO9dvcK8ddnJEvQk6cqqOctXniEuERYIQJ-2uWVXfmVR3sizlAr32pl_KtlB-daDxrfiLel_AAAqZw7FadeolLenomrTU-Zusp5-E11SHOTYlP6lGtTad7y1GTeM9XSxJympvZDro9tmrnpDxPNTeUxTZy5XIjXkcbC747zXNx_-3q3uWpvbr9fb77ctJ0CWVuNSORsB854ksjg1sZKUvQAkWIXndIx8tpolrQFVCbKrQKktUZtJJA8F5-f_-4PDxNvO55rpjHs8zBRfgyJhvDvZR76sEu_gkQwxi35D8d8Tj8PXGqYhtLxONLM6VCC9RK8lfa_IJq1Xji_gB-fwS6nUjLHlzII4Y-fsMvBBh8WPwv8_u_6L-hRiHwC8e-M7g</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Rosenberg, M</creator><creator>Hui, L</creator><creator>Ma, J</creator><creator>Nusbaum, H C</creator><creator>Clark, K</creator><creator>Robinson, L</creator><creator>Dziadzio, L</creator><creator>Swain, P M</creator><creator>Keith, T</creator><creator>Hudson, T J</creator><creator>Biesecker, L G</creator><creator>Flint, J</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970901</creationdate><title>Characterization of short tandem repeats from thirty-one human telomeres</title><author>Rosenberg, M ; Hui, L ; Ma, J ; Nusbaum, H C ; Clark, K ; Robinson, L ; Dziadzio, L ; Swain, P M ; Keith, T ; Hudson, T J ; Biesecker, L G ; Flint, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-511aa87c0869a31e082673a4ab0fafcf845ffe265e3ad0146f3d401a2515630a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Base Sequence</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human - genetics</topic><topic>Humans</topic><topic>Letters</topic><topic>Molecular Sequence Data</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Tagged Sites</topic><topic>Telomere - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosenberg, M</creatorcontrib><creatorcontrib>Hui, L</creatorcontrib><creatorcontrib>Ma, J</creatorcontrib><creatorcontrib>Nusbaum, H C</creatorcontrib><creatorcontrib>Clark, K</creatorcontrib><creatorcontrib>Robinson, L</creatorcontrib><creatorcontrib>Dziadzio, L</creatorcontrib><creatorcontrib>Swain, P M</creatorcontrib><creatorcontrib>Keith, T</creatorcontrib><creatorcontrib>Hudson, T J</creatorcontrib><creatorcontrib>Biesecker, L G</creatorcontrib><creatorcontrib>Flint, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosenberg, M</au><au>Hui, L</au><au>Ma, J</au><au>Nusbaum, H C</au><au>Clark, K</au><au>Robinson, L</au><au>Dziadzio, L</au><au>Swain, P M</au><au>Keith, T</au><au>Hudson, T J</au><au>Biesecker, L G</au><au>Flint, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of short tandem repeats from thirty-one human telomeres</atitle><jtitle>Genome research</jtitle><addtitle>Genome Res</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>7</volume><issue>9</issue><spage>917</spage><epage>923</epage><pages>917-923</pages><issn>1088-9051</issn><eissn>1549-5469</eissn><abstract>Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>9314497</pmid><doi>10.1101/gr.7.9.917</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1088-9051 |
| ispartof | Genome research, 1997-09, Vol.7 (9), p.917-923 |
| issn | 1088-9051 1549-5469 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_310668 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Base Sequence Chromosome Mapping Chromosomes, Human - genetics Humans Letters Molecular Sequence Data Repetitive Sequences, Nucleic Acid - genetics Sequence Analysis, DNA Sequence Tagged Sites Telomere - genetics |
| title | Characterization of short tandem repeats from thirty-one human telomeres |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T01%3A45%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20short%20tandem%20repeats%20from%20thirty-one%20human%20telomeres&rft.jtitle=Genome%20research&rft.au=Rosenberg,%20M&rft.date=1997-09-01&rft.volume=7&rft.issue=9&rft.spage=917&rft.epage=923&rft.pages=917-923&rft.issn=1088-9051&rft.eissn=1549-5469&rft_id=info:doi/10.1101/gr.7.9.917&rft_dat=%3Cproquest_pubme%3E16257379%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16257379&rft_id=info:pmid/9314497&rfr_iscdi=true |