Contacts between 16S ribosomal RNA and mRNA, within the spacer region separating the AUG initiator codon and the Shine -Dalgarno sequence; a site-directed cross-linking study

mRNA analogues containing several 4-thiouridine (thio- U) residues at selected positions were prepared by T7-transcription. The spacer region between the Shine-Dalgarno sequence and the AUG codon consisted of four or eight bases with a single thio-U at a variable position; alternatively, cro-mRNA an...

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Veröffentlicht in:	Nucleic acids research 1994-08, Vol.22 (15), p.3018-3025
Hauptverfasser:	Rinke-Appel, Jutta, Jünke, Nicole, Brimacombe, Richard, Lavrik, Inna, Dokudovskaya, Svetlana, Dontsova, Olga, Bogdanov, Alexei
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Codon Cross-Linking Reagents Escherichia coli Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation Ribonuclease H - metabolism RNA, Messenger - chemistry RNA, Messenger - metabolism RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - metabolism RNA, Transfer - metabolism RNA, Transfer, Glu - metabolism RNA, Transfer, Met - metabolism T-Phages - genetics Thiouracil Transcription, Genetic Translation. Translation factors. Protein processing
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