UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation
Efficient splicing of higher plant pre‐mRNAs depends on AU‐ or U‐rich sequences in introns. Moreover, AU‐rich sequences present in 3′‐untranslated regions (3′‐UTRs) may play a role in 3′ end processing of plant mRNAs. Here, we describe the cloning and characterization of a Nicotiana plumbaginifolia...
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| Veröffentlicht in: | The EMBO journal 2000-04, Vol.19 (7), p.1638-1649 |
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| creator | Lambermon, M.H.L Simpson, G.G Wieczorek Kirk, D.A Hemmings-Mieszczak, M Klahre, U Filipowicz, W |
| description | Efficient splicing of higher plant pre‐mRNAs depends on AU‐ or U‐rich sequences in introns. Moreover, AU‐rich sequences present in 3′‐untranslated regions (3′‐UTRs) may play a role in 3′ end processing of plant mRNAs. Here, we describe the cloning and characterization of a
Nicotiana plumbaginifolia
nuclear protein that can be cross‐linked to U‐rich intron and 3′‐UTR sequences
in vitro
, and associates with nuclear poly(A)
+
RNA
in vivo
. The protein, UBP1, strongly enhances the splicing of otherwise inefficiently processed introns when overexpressed in protoplasts. It also increases the accumulation of reporter mRNAs that contain suboptimal introns or are intronless. The enhanced accumulation is apparently due to UBP1 interacting with the 3′‐UTR and protecting mRNA from exonucleolytic degradation. The effect on mRNA accumulation but not on mRNA splicing was found to be promoter specific. The fact that these effects of UBP1 can be separated suggests that they represent two independent activities. The properties of UBP1 indicate that it is an hnRNP protein that functions at multiple steps to facilitate the nuclear maturation of plant pre‐mRNAs. |
| doi_str_mv | 10.1093/emboj/19.7.1638 |
| format | Article |
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Nicotiana plumbaginifolia
nuclear protein that can be cross‐linked to U‐rich intron and 3′‐UTR sequences
in vitro
, and associates with nuclear poly(A)
+
RNA
in vivo
. The protein, UBP1, strongly enhances the splicing of otherwise inefficiently processed introns when overexpressed in protoplasts. It also increases the accumulation of reporter mRNAs that contain suboptimal introns or are intronless. The enhanced accumulation is apparently due to UBP1 interacting with the 3′‐UTR and protecting mRNA from exonucleolytic degradation. The effect on mRNA accumulation but not on mRNA splicing was found to be promoter specific. The fact that these effects of UBP1 can be separated suggests that they represent two independent activities. The properties of UBP1 indicate that it is an hnRNP protein that functions at multiple steps to facilitate the nuclear maturation of plant pre‐mRNAs.</description><identifier>ISSN: 0261-4189</identifier><identifier>ISSN: 1460-2075</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1093/emboj/19.7.1638</identifier><identifier>PMID: 10747031</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>3'-untranslated region ; 3′-UTR ; Accumulation ; actin ; alternative splicing ; Amino Acid Sequence ; amino acid sequences ; binding ; binding proteins ; Cloning ; Cloning, Molecular ; complementary DNA ; genbank/aj272011 ; Gene Expression ; genes ; Genes, Plant ; Heterogeneous-Nuclear Ribonucleoproteins ; hnRNA ; hnRNP proteins ; immunocytochemistry ; Introns ; messenger RNA ; Molecular Sequence Data ; mRNA splicing ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana plumbaginifolia ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; nuclei ; nucleoproteins ; nucleotide sequences ; Plant Proteins - genetics ; Plant Proteins - metabolism ; plant RNA processing ; Plants, Toxic ; poly(a)+ rna ; precursors ; promoter regions ; protoplasts ; ribonucleoproteins ; Ribonucleoproteins - genetics ; Ribonucleoproteins - metabolism ; RNA Precursors - metabolism ; RNA Splicing ; RNA, Plant - metabolism ; RNA-binding protein ; RNA-binding proteins ; Sequence Homology, Amino Acid ; suboptimal introns ; UBP1 protein ; Zea mays</subject><ispartof>The EMBO journal, 2000-04, Vol.19 (7), p.1638-1649</ispartof><rights>European Molecular Biology Organization 2000</rights><rights>Copyright © 2000 European Molecular Biology Organization</rights><rights>Copyright Oxford University Press(England) Apr 03, 2000</rights><rights>Copyright © 2000 European Molecular Biology Organization 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6205-f031d128f46fda7334861e364bcc3e150e7817020a8d132fd30153f715b6045d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC310232/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC310232/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10747031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lambermon, M.H.L</creatorcontrib><creatorcontrib>Simpson, G.G</creatorcontrib><creatorcontrib>Wieczorek Kirk, D.A</creatorcontrib><creatorcontrib>Hemmings-Mieszczak, M</creatorcontrib><creatorcontrib>Klahre, U</creatorcontrib><creatorcontrib>Filipowicz, W</creatorcontrib><title>UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><addtitle>EMBO J</addtitle><description>Efficient splicing of higher plant pre‐mRNAs depends on AU‐ or U‐rich sequences in introns. Moreover, AU‐rich sequences present in 3′‐untranslated regions (3′‐UTRs) may play a role in 3′ end processing of plant mRNAs. Here, we describe the cloning and characterization of a
Nicotiana plumbaginifolia
nuclear protein that can be cross‐linked to U‐rich intron and 3′‐UTR sequences
in vitro
, and associates with nuclear poly(A)
+
RNA
in vivo
. The protein, UBP1, strongly enhances the splicing of otherwise inefficiently processed introns when overexpressed in protoplasts. It also increases the accumulation of reporter mRNAs that contain suboptimal introns or are intronless. The enhanced accumulation is apparently due to UBP1 interacting with the 3′‐UTR and protecting mRNA from exonucleolytic degradation. The effect on mRNA accumulation but not on mRNA splicing was found to be promoter specific. The fact that these effects of UBP1 can be separated suggests that they represent two independent activities. The properties of UBP1 indicate that it is an hnRNP protein that functions at multiple steps to facilitate the nuclear maturation of plant pre‐mRNAs.</description><subject>3'-untranslated region</subject><subject>3′-UTR</subject><subject>Accumulation</subject><subject>actin</subject><subject>alternative splicing</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>binding</subject><subject>binding proteins</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>genbank/aj272011</subject><subject>Gene Expression</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Heterogeneous-Nuclear Ribonucleoproteins</subject><subject>hnRNA</subject><subject>hnRNP proteins</subject><subject>immunocytochemistry</subject><subject>Introns</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>mRNA splicing</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana plumbaginifolia</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>nuclei</subject><subject>nucleoproteins</subject><subject>nucleotide sequences</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>plant RNA processing</subject><subject>Plants, Toxic</subject><subject>poly(a)+ rna</subject><subject>precursors</subject><subject>promoter regions</subject><subject>protoplasts</subject><subject>ribonucleoproteins</subject><subject>Ribonucleoproteins - genetics</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA Precursors - metabolism</subject><subject>RNA Splicing</subject><subject>RNA, Plant - metabolism</subject><subject>RNA-binding protein</subject><subject>RNA-binding proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>suboptimal introns</subject><subject>UBP1 protein</subject><subject>Zea mays</subject><issn>0261-4189</issn><issn>1460-2075</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFks9v0zAYhiMEYmNw5gRYHDiR1p8dx8mBwzrG-LGVaVDYzXITp3WX2MFOBvvvcchUChLiFFnf83x5rddR9BjwBHBOp6pZ2s0U8gmfQEqzO9E-JCmOCebsbrSPSQpxAlm-Fz3wfoMxZhmH-9EeYJ5wTGE_ahazc3iJJDL2WtVobS7m53GtrxRqne2UNqhbyw5VvSk6bY1H4dD0dafbWiHfqdYjW6G1Xq2VQ20tTYdMX9RKhpNTcXMxP0SN7HonB_1hdK-StVePbr8H0eLN8eejt_Hpx5N3R4encZESzOIqRCuBZFWSVqXklCZZCoqmybIoqAKGFc-AY4JlVgIlVUkxMFpxYMsUJ6ykB9GrcW_bLxtVFsp0TtaidbqR7kZYqcWfE6PXYmWvBQVMKAn-i1vf2W-98p1otC9UHe6nbO8FcEZplrEAPv8L3NjemXA3ATkjKaNsgKYjVDjrvVPVNghgMdQoftUYDMHFUGMwnu7m3-HH3gKQjcB3Xaub_-0Tx2ez95zlhPAhDR5VHyyzUm4n8j_jPBkVMzSptr_7vTIe5zq8iB_bsXRXIuWUM_F1fiJef5ldfpifEXEZ-GcjX0kr5MppLxafCAaKSZ5kFGP6E2UM3TM</recordid><startdate>20000403</startdate><enddate>20000403</enddate><creator>Lambermon, M.H.L</creator><creator>Simpson, G.G</creator><creator>Wieczorek Kirk, D.A</creator><creator>Hemmings-Mieszczak, M</creator><creator>Klahre, U</creator><creator>Filipowicz, W</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Blackwell Publishing Ltd</general><general>Oxford University 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a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation</title><author>Lambermon, M.H.L ; Simpson, G.G ; Wieczorek Kirk, D.A ; Hemmings-Mieszczak, M ; Klahre, U ; Filipowicz, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6205-f031d128f46fda7334861e364bcc3e150e7817020a8d132fd30153f715b6045d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>3'-untranslated region</topic><topic>3′-UTR</topic><topic>Accumulation</topic><topic>actin</topic><topic>alternative splicing</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>binding</topic><topic>binding proteins</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>genbank/aj272011</topic><topic>Gene Expression</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Heterogeneous-Nuclear Ribonucleoproteins</topic><topic>hnRNA</topic><topic>hnRNP proteins</topic><topic>immunocytochemistry</topic><topic>Introns</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>mRNA splicing</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana plumbaginifolia</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>nuclei</topic><topic>nucleoproteins</topic><topic>nucleotide sequences</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>plant RNA processing</topic><topic>Plants, Toxic</topic><topic>poly(a)+ rna</topic><topic>precursors</topic><topic>promoter regions</topic><topic>protoplasts</topic><topic>ribonucleoproteins</topic><topic>Ribonucleoproteins - genetics</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA Precursors - metabolism</topic><topic>RNA Splicing</topic><topic>RNA, Plant - metabolism</topic><topic>RNA-binding protein</topic><topic>RNA-binding proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>suboptimal introns</topic><topic>UBP1 protein</topic><topic>Zea mays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lambermon, M.H.L</creatorcontrib><creatorcontrib>Simpson, G.G</creatorcontrib><creatorcontrib>Wieczorek Kirk, D.A</creatorcontrib><creatorcontrib>Hemmings-Mieszczak, M</creatorcontrib><creatorcontrib>Klahre, U</creatorcontrib><creatorcontrib>Filipowicz, W</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology 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titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lambermon, M.H.L</au><au>Simpson, G.G</au><au>Wieczorek Kirk, D.A</au><au>Hemmings-Mieszczak, M</au><au>Klahre, U</au><au>Filipowicz, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation</atitle><jtitle>The EMBO journal</jtitle><stitle>EMBO J</stitle><addtitle>EMBO J</addtitle><date>2000-04-03</date><risdate>2000</risdate><volume>19</volume><issue>7</issue><spage>1638</spage><epage>1649</epage><pages>1638-1649</pages><issn>0261-4189</issn><issn>1460-2075</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>Efficient splicing of higher plant pre‐mRNAs depends on AU‐ or U‐rich sequences in introns. Moreover, AU‐rich sequences present in 3′‐untranslated regions (3′‐UTRs) may play a role in 3′ end processing of plant mRNAs. Here, we describe the cloning and characterization of a
Nicotiana plumbaginifolia
nuclear protein that can be cross‐linked to U‐rich intron and 3′‐UTR sequences
in vitro
, and associates with nuclear poly(A)
+
RNA
in vivo
. The protein, UBP1, strongly enhances the splicing of otherwise inefficiently processed introns when overexpressed in protoplasts. It also increases the accumulation of reporter mRNAs that contain suboptimal introns or are intronless. The enhanced accumulation is apparently due to UBP1 interacting with the 3′‐UTR and protecting mRNA from exonucleolytic degradation. The effect on mRNA accumulation but not on mRNA splicing was found to be promoter specific. The fact that these effects of UBP1 can be separated suggests that they represent two independent activities. The properties of UBP1 indicate that it is an hnRNP protein that functions at multiple steps to facilitate the nuclear maturation of plant pre‐mRNAs.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10747031</pmid><doi>10.1093/emboj/19.7.1638</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The EMBO journal, 2000-04, Vol.19 (7), p.1638-1649 |
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| source | MEDLINE; Wiley Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 3'-untranslated region 3′-UTR Accumulation actin alternative splicing Amino Acid Sequence amino acid sequences binding binding proteins Cloning Cloning, Molecular complementary DNA genbank/aj272011 Gene Expression genes Genes, Plant Heterogeneous-Nuclear Ribonucleoproteins hnRNA hnRNP proteins immunocytochemistry Introns messenger RNA Molecular Sequence Data mRNA splicing Nicotiana - genetics Nicotiana - metabolism Nicotiana plumbaginifolia Nuclear Proteins - genetics Nuclear Proteins - metabolism nuclei nucleoproteins nucleotide sequences Plant Proteins - genetics Plant Proteins - metabolism plant RNA processing Plants, Toxic poly(a)+ rna precursors promoter regions protoplasts ribonucleoproteins Ribonucleoproteins - genetics Ribonucleoproteins - metabolism RNA Precursors - metabolism RNA Splicing RNA, Plant - metabolism RNA-binding protein RNA-binding proteins Sequence Homology, Amino Acid suboptimal introns UBP1 protein Zea mays |
| title | UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation |
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