Systems Biology Approach in Chlamydomonas Reveals Connections between Copper Nutrition and Multiple Metabolic Steps

In this work, we query the Chlamydomonas reinhardtii copper regulon at a whole-genome level. Our RNA-Seq data simulation and analysis pipeline validated a 2-fold cutoff and 10 RPKM (reads per kilobase of mappable length per million mapped reads) (~1 mRNA per cell) to reveal 63 CRR1 targets plus anot...

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Veröffentlicht in:	The Plant cell 2011-04, Vol.23 (4), p.1273-1292
Hauptverfasser:	Castruita, Madeli, Casero, David, Karpowicz, Steven J., Kropat, Janette, Vieler, Astrid, Hsieh, Scott I., Yan, Weihong, Cokus, Shawn, Loo, Joseph A., Benning, Christoph, Pellegrini, Matteo, Merchant, Sabeeha S.
Format:	Artikel
Sprache:	eng
Schlagworte:	amines Autotrophic Processes - genetics Base Sequence Chaperones Chlamydomonas Chlamydomonas - genetics Chlamydomonas - metabolism Chlamydomonas reinhardtii Chloroplasts Copper Copper - deficiency Copper - metabolism Cytochromes Data processing Datasets Enzymes Fatty acids Gene Expression Profiling Gene Expression Regulation, Plant Gene regulation Genes Genes, Plant - genetics Genetic Loci - genetics Genomes Heterotrophic Processes - genetics Lipids Merchants Messenger RNA Metabolism - genetics Molecular Sequence Data Nutrition Nutritional Physiological Phenomena - genetics Oxidases Oxidation-Reduction Photosynthetic apparatus Plant cells Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plastids Promoter Regions, Genetic - genetics Protein Structure, Tertiary Proteome - metabolism proteomics Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Plant - genetics RNA, Plant - metabolism Signal transduction Systems Biology Thylakoid membranes Thylakoids Transcription
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