Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen
Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stro...
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| Veröffentlicht in: | Vaccine 2011-02, Vol.29 (6), p.1258-1269 |
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| container_title | Vaccine |
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| creator | Arias, Mauricio A Loxley, Andrew Eatmon, Christy Van Roey, Griet Fairhurst, David Mitchnick, Mark Dash, Philip Cole, Tom Wegmann, Frank Sattentau, Quentin Shattock, Robin |
| description | Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates. |
| doi_str_mv | 10.1016/j.vaccine.2010.11.084 |
| format | Article |
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We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.11.084</identifier><identifier>PMID: 21145913</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>adjuvants ; Adjuvants, Immunologic - pharmacology ; AIDS Vaccines - immunology ; Allergy and Immunology ; Animals ; Antigens ; Applied microbiology ; Biological and medical sciences ; blood serum ; carnauba wax ; Cell Proliferation ; Dendritic Cells - immunology ; Drug Carriers - administration & dosage ; env Gene Products, Human Immunodeficiency Virus - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; gp140 antigen ; HIV ; HIV Antibodies - blood ; HIV Infections - prevention & control ; Human immunodeficiency virus ; Humans ; humoral immunity ; immune response ; Immune system ; Immunity, Mucosal ; Immunization ; Immunogenicity ; immunoglobulin A ; Immunoglobulin A - analysis ; immunoglobulin G ; Immunoglobulin G - blood ; inflammation ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mucosal immunity ; Nanoparticles ; Nanoparticles - administration & dosage ; Particle size ; Product development ; Studies ; T-lymphocytes ; T-Lymphocytes - immunology ; Tetanus ; vaccination ; vaccine development ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vagina - immunology ; Virology ; Waxes - pharmacology</subject><ispartof>Vaccine, 2011-02, Vol.29 (6), p.1258-1269</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Feb 1, 2011</rights><rights>2011 Elsevier Ltd. 2010 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-374faa19a4a5d691ed23cd69a958366907cdca44044441477e84068f85564a6c3</citedby><cites>FETCH-LOGICAL-c668t-374faa19a4a5d691ed23cd69a958366907cdca44044441477e84068f85564a6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X10017287$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23860981$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21145913$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arias, Mauricio A</creatorcontrib><creatorcontrib>Loxley, Andrew</creatorcontrib><creatorcontrib>Eatmon, Christy</creatorcontrib><creatorcontrib>Van Roey, Griet</creatorcontrib><creatorcontrib>Fairhurst, David</creatorcontrib><creatorcontrib>Mitchnick, Mark</creatorcontrib><creatorcontrib>Dash, Philip</creatorcontrib><creatorcontrib>Cole, Tom</creatorcontrib><creatorcontrib>Wegmann, Frank</creatorcontrib><creatorcontrib>Sattentau, Quentin</creatorcontrib><creatorcontrib>Shattock, Robin</creatorcontrib><title>Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates.</description><subject>adjuvants</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>AIDS Vaccines - immunology</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antigens</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>carnauba wax</subject><subject>Cell Proliferation</subject><subject>Dendritic Cells - immunology</subject><subject>Drug Carriers - administration & dosage</subject><subject>env Gene Products, Human Immunodeficiency Virus - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gp140 antigen</subject><subject>HIV</subject><subject>HIV Antibodies - blood</subject><subject>HIV Infections - prevention & control</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>humoral immunity</subject><subject>immune response</subject><subject>Immune system</subject><subject>Immunity, Mucosal</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>immunoglobulin A</subject><subject>Immunoglobulin A - analysis</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>inflammation</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mucosal immunity</subject><subject>Nanoparticles</subject><subject>Nanoparticles - administration & dosage</subject><subject>Particle size</subject><subject>Product development</subject><subject>Studies</subject><subject>T-lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>Tetanus</subject><subject>vaccination</subject><subject>vaccine development</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vagina - immunology</subject><subject>Virology</subject><subject>Waxes - pharmacology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk9v1DAQxSMEokvhIwCREIJLFjv-E-dShFZAK1XiUIq4WVPH2fWS2Fs7Wdhvz4RdWugBfIk1-fn5jedl2VNK5pRQ-WY934Ixztt5SaYanRPF72UzqipWlIKq-9mMlJIXnJKvR9mjlNaEEMFo_TA7KinloqZslu0WED2MV5B_hx-5Bx82EAdnOpty61fgjc3TEINf5mmXBts7k4Nv8n40IUGXG9t1YwfxV3E19iFi0fX96G0ebdoEn1BpCPnp2ZdiuaGcIDm4pfWPswctdMk-OXyPs8sP7z8vTovzTx_PFu_OCyOlGgpW8RaA1sBBNLKmtimZwQ3UQjEpa1KZxgDnhOOivKqs4kSqVgkhOUjDjrOTve5mvOptY6wf0KPeRNdD3OkATv_9x7uVXoatZqRWnNUo8OogEMP1aNOge5emvsHbMCatJK-FLDlB8vU_SSorhZMpywl9cQddhxEn0SElJJFCKKKQEnvKxJBStO2NbUr0FAO91ocY6CkGmlKNMcBzz_7s-ebU77kj8PIAQDLQtREH7dItx5TE7ilyz_dcC0HDMiJzeYE3CUIoUxWfXuftnrA4w62zUSfjLMamcdGaQTfB_dfsyR0F0znv0NY3u7Pp9l10KjXRF1Oqp1BT9FCVGPefLwTxww</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Arias, Mauricio A</creator><creator>Loxley, Andrew</creator><creator>Eatmon, Christy</creator><creator>Van Roey, Griet</creator><creator>Fairhurst, David</creator><creator>Mitchnick, Mark</creator><creator>Dash, Philip</creator><creator>Cole, Tom</creator><creator>Wegmann, Frank</creator><creator>Sattentau, Quentin</creator><creator>Shattock, Robin</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><general>Elsevier Science</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20110201</creationdate><title>Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen</title><author>Arias, Mauricio A ; Loxley, Andrew ; Eatmon, Christy ; Van Roey, Griet ; Fairhurst, David ; Mitchnick, Mark ; Dash, Philip ; Cole, Tom ; Wegmann, Frank ; Sattentau, Quentin ; Shattock, Robin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-374faa19a4a5d691ed23cd69a958366907cdca44044441477e84068f85564a6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adjuvants</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>AIDS Vaccines - immunology</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antigens</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>carnauba wax</topic><topic>Cell Proliferation</topic><topic>Dendritic Cells - immunology</topic><topic>Drug Carriers - administration & dosage</topic><topic>env Gene Products, Human Immunodeficiency Virus - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gp140 antigen</topic><topic>HIV</topic><topic>HIV Antibodies - blood</topic><topic>HIV Infections - prevention & control</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>humoral immunity</topic><topic>immune response</topic><topic>Immune system</topic><topic>Immunity, Mucosal</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>immunoglobulin A</topic><topic>Immunoglobulin A - analysis</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>inflammation</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mucosal immunity</topic><topic>Nanoparticles</topic><topic>Nanoparticles - administration & dosage</topic><topic>Particle size</topic><topic>Product development</topic><topic>Studies</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>Tetanus</topic><topic>vaccination</topic><topic>vaccine development</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vagina - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arias, Mauricio A</au><au>Loxley, Andrew</au><au>Eatmon, Christy</au><au>Van Roey, Griet</au><au>Fairhurst, David</au><au>Mitchnick, Mark</au><au>Dash, Philip</au><au>Cole, Tom</au><au>Wegmann, Frank</au><au>Sattentau, Quentin</au><au>Shattock, Robin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>29</volume><issue>6</issue><spage>1258</spage><epage>1269</epage><pages>1258-1269</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Induction of humoral responses to HIV at mucosal compartments without inflammation is important for vaccine design. We developed charged wax nanoparticles that efficiently adsorb protein antigens and are internalized by DC in the absence of inflammation. HIV-gp140-adsorbed nanoparticles induced stronger in vitro T-cell proliferation responses than antigen alone. Such responses were greatly enhanced when antigen was co-adsorbed with TLR ligands. Immunogenicity studies in mice showed that intradermal vaccination with HIV-gp140 antigen-adsorbed nanoparticles induced high levels of specific IgG. Importantly, intranasal immunization with HIV-gp140-adsorbed nanoparticles greatly enhanced serum and vaginal IgG and IgA responses. Our results show that HIV-gp140-carrying wax nanoparticles can induce strong cellular/humoral immune responses without inflammation and may be of potential use as effective mucosal adjuvants for HIV vaccine candidates.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21145913</pmid><doi>10.1016/j.vaccine.2010.11.084</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Vaccine, 2011-02, Vol.29 (6), p.1258-1269 |
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| subjects | adjuvants Adjuvants, Immunologic - pharmacology AIDS Vaccines - immunology Allergy and Immunology Animals Antigens Applied microbiology Biological and medical sciences blood serum carnauba wax Cell Proliferation Dendritic Cells - immunology Drug Carriers - administration & dosage env Gene Products, Human Immunodeficiency Virus - immunology Female Fundamental and applied biological sciences. Psychology gp140 antigen HIV HIV Antibodies - blood HIV Infections - prevention & control Human immunodeficiency virus Humans humoral immunity immune response Immune system Immunity, Mucosal Immunization Immunogenicity immunoglobulin A Immunoglobulin A - analysis immunoglobulin G Immunoglobulin G - blood inflammation Mice Mice, Inbred BALB C Microbiology Miscellaneous Mucosal immunity Nanoparticles Nanoparticles - administration & dosage Particle size Product development Studies T-lymphocytes T-Lymphocytes - immunology Tetanus vaccination vaccine development Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vagina - immunology Virology Waxes - pharmacology |
| title | Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen |
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