Comparison of Schizosaccharomyces pombe expression systems
Analysis of a variety of problems in yeast relies on ectopic expression of the protein of interest under control of a heterologous promoter. In the fission yeast S. pombe , there are relatively few expression plasmids and most reports of their activity have not been comparative. It is thus difficult...
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| Veröffentlicht in: | Nucleic acids research 1993-06, Vol.21 (12), p.2955-2956 |
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| container_title | Nucleic acids research |
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| creator | Forsburg, S.L |
| description | Analysis of a variety of problems in yeast relies on ectopic expression of the protein of interest under control of a heterologous promoter. In the fission yeast S. pombe , there are relatively few expression plasmids and most reports of their activity have not been comparative. It is thus difficult to determine the comparability of experiments carried out using different expression vectors. When new promoters are characterised, it is likewise difficult to determine their activity relative to those previously identified. In order to better assess relative promoter strengths, I have compared the activity of several inducible and constitutive expression systems in fission yeast by using a beta-galactosidase reporter gene. This allows a more informed choice of appropriate expression systems for analysis of gene function in S. pombe . |
| doi_str_mv | 10.1093/nar/21.12.2955 |
| format | Article |
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In the fission yeast S. pombe , there are relatively few expression plasmids and most reports of their activity have not been comparative. It is thus difficult to determine the comparability of experiments carried out using different expression vectors. When new promoters are characterised, it is likewise difficult to determine their activity relative to those previously identified. In order to better assess relative promoter strengths, I have compared the activity of several inducible and constitutive expression systems in fission yeast by using a beta-galactosidase reporter gene. This allows a more informed choice of appropriate expression systems for analysis of gene function in S. pombe .</description><subject>beta-galactosidase</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Fungal</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>genetic transformation</subject><subject>Methods. Procedures. Technologies</subject><subject>plasmid vectors</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant DNA</subject><subject>reporter genes</subject><subject>Repressor Proteins - genetics</subject><subject>Saccharomycetales</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Tetracycline</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1v00AQxVcIVELhyg2RA-LmdGa_7EXiABE0SBUcSiXEZTVerxuD7TW7CWr469koUQSnnubwfm8-9Iax5wgLBCMuRooXHBfIF9wo9YDNUGheSKP5QzYDAapAkNVj9iSlHwAoUckzdlYJwRXqGXuzDMNEsUthnId2fu3W3Z-QyLk1xTDsnE_zKQy1n_u7KfqUusylXdr4IT1lj1rqk392rOfs5uOHr8tVcfXl8tPy3VXhpCk3BYFQBE5qzSXwkpPRBE3jDGmH5EVd1i15A17XniuliLAx3EOrteRNrcQ5e3voO23rwTfOj5tIvZ1iN1Dc2UCd_V8Zu7W9Db-tAFOCzv7XR38Mv7Y-bezQJef7nkYftsmWqpLCQHkvyDOTN8R7QdS5ZSn3oxcH0MWQUvTtaWsEu4_P5vgsR4vc7uPLhhf_3nrCj3ll_dVRp-SobyONrksnTFaYqf0lxQHrclJ3J5niT5vVUtnVt-92JarP7y-X2kLmXx74loKl2_wO9uaaA4r8MBUojeIvkFa8aA</recordid><startdate>19930625</startdate><enddate>19930625</enddate><creator>Forsburg, S.L</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930625</creationdate><title>Comparison of Schizosaccharomyces pombe expression systems</title><author>Forsburg, S.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-a035a0c466240272a96a0ddc9a6c1ae3b7bfae90e6be2555aa1d92e0f6642db53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>beta-galactosidase</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Fungal</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>genetic transformation</topic><topic>Methods. Procedures. Technologies</topic><topic>plasmid vectors</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant DNA</topic><topic>reporter genes</topic><topic>Repressor Proteins - genetics</topic><topic>Saccharomycetales</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Tetracycline</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forsburg, S.L</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forsburg, S.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Schizosaccharomyces pombe expression systems</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1993-06-25</date><risdate>1993</risdate><volume>21</volume><issue>12</issue><spage>2955</spage><epage>2956</epage><pages>2955-2956</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>Analysis of a variety of problems in yeast relies on ectopic expression of the protein of interest under control of a heterologous promoter. In the fission yeast S. pombe , there are relatively few expression plasmids and most reports of their activity have not been comparative. It is thus difficult to determine the comparability of experiments carried out using different expression vectors. When new promoters are characterised, it is likewise difficult to determine their activity relative to those previously identified. In order to better assess relative promoter strengths, I have compared the activity of several inducible and constitutive expression systems in fission yeast by using a beta-galactosidase reporter gene. This allows a more informed choice of appropriate expression systems for analysis of gene function in S. pombe .</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>8332516</pmid><doi>10.1093/nar/21.12.2955</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1993-06, Vol.21 (12), p.2955-2956 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_309706 |
| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | beta-galactosidase beta-Galactosidase - genetics Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Gene Expression Genes, Fungal Genetic engineering Genetic technics genetic transformation Methods. Procedures. Technologies plasmid vectors promoter regions Promoter Regions, Genetic recombinant DNA reporter genes Repressor Proteins - genetics Saccharomycetales Schizosaccharomyces - genetics Schizosaccharomyces pombe Tetracycline Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Comparison of Schizosaccharomyces pombe expression systems |
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