Determination of the molecular mass of the transcription factor interacting with FBS2 in c-fos promoter
Eucaryotic genes are regulated by transcriptional factors binding to specific nucleotide sequences within the promoter regions of genes. These factors are present in a small copy number in the cells, and the purification and characterization of these proteins is a laborious task. To identify polypep...
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| Veröffentlicht in: | Nucleic acids research 1993-05, Vol.21 (10), p.2535-2536 |
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| container_issue | 10 |
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| container_title | Nucleic acids research |
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| creator | Svinarchuk, F. Mastyugin, V. Gorn, V. Dobrikov, M. Doronin, S. |
| description | Eucaryotic genes are regulated by transcriptional factors binding to specific nucleotide sequences within the promoter regions of genes. These factors are present in a small copy number in the cells, and the purification and characterization of these proteins is a laborious task. To identify polypeptides interacting with FBS-2 site in nucleic extracts we used the modification of the known method of super(32)P-labeling of the polypeptides by crosslinking to radioactive DNA probes containing photoactivatable derivatives of nucleotides. The photoactivatable analogs were 5-BrdU (5-bromodeoxyuridine-5'-monophosphate) or FABdC (4N-(2-(p-azidotetrafluoro-benzamido)ethyl 1))-deoxycytidine). |
| doi_str_mv | 10.1093/nar/21.10.2535 |
| format | Article |
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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1993-05, Vol.21 (10), p.2535-2536 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_309573 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Affinity Labels Base Sequence Binding Sites DNA - metabolism DNA Probes Genes, fos Molecular Sequence Data Molecular Weight Promoter Regions, Genetic Transcription Factors - chemistry Transcription Factors - metabolism |
| title | Determination of the molecular mass of the transcription factor interacting with FBS2 in c-fos promoter |
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