Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy
The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels....
Gespeichert in:
| Veröffentlicht in: | Biophysical journal 2011-05, Vol.100 (10), p.L53-L55 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | L55 |
|---|---|
| container_issue | 10 |
| container_start_page | L53 |
| container_title | Biophysical journal |
| container_volume | 100 |
| creator | McNary, Thomas G. Bridge, John H.B. Sachse, Frank B. |
| description | The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels. Here, we investigated mechanical modulation of the t-system. By applying fluorescent markers, three-dimensional scanning confocal microscopy, and methods of digital image analysis, we studied isolated ventricular cardiomyocytes under different strains. We demonstrate that strain at the cellular level is transmitted to the t-system, reducing the length and volume of tubules and altering their cross-sectional shape. Our data suggest that a cellular strain of as little as 5% affects the shape of transverse tubules, which has important implications for the function of mechanosensitive ion channels found in them. Furthermore, our study supports a prior hypothesis that strain can cause fluid exchange between the t-system and extracellular space. |
| doi_str_mv | 10.1016/j.bpj.2011.03.046 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3093556</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006349511004048</els_id><sourcerecordid>867474676</sourcerecordid><originalsourceid>FETCH-LOGICAL-c501t-37d5f51ecc4dba57061ec5c24a4adb607847e93ccc50ea1f5a45485ce0d9d2673</originalsourceid><addsrcrecordid>eNp9kV-L1DAUxYso7rj6AXzR4ItPrTdt0rQIggz-gxXBmfU1pOntbEonmU3agX57U7su6oNPuZDfPdxzTpI8p5BRoOWbPmtOfZYDpRkUGbDyQbKhnOUpQFU-TDYAUKYFq_lF8iSEHoDmHOjj5CKnXHBesk0y70avjCV7r2zo0JM4_0A7eqOnQXmyVb417jg7PY8YyOjI_gaNX_kz-oBkPzW_0N0cRjyS73hGNWBLmpnstLLW2APZOts5rQby1Wjvgnan-WnyqFNDwGd372Vy_fHDfvs5vfr26cv2_VWq461jWoiWd5yi1qxtFBdQxpnrnCmm2qYEUTGBdaF1xFHRjivGWcU1Qlu3eSmKy-TdqnuamiO2ejGnBnny5qj8LJ0y8u8fa27kwZ1lAXURQ4oCr-8EvLudMIzyaILGYVAW3RRkVQomWCkW8tU_ZO8mb6M7WQkKRZ3XLEJ0hZYggsfu_hQKcqlV9jLWKpdaJRQy1hp3Xvzp4X7jd48ReLkCnXJSHbwJ8noXFXjsnNIKFom3K4Ex67NBL4M2aDW2xqMeZevMfw74CadUv0E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>871039294</pqid></control><display><type>article</type><title>Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>McNary, Thomas G. ; Bridge, John H.B. ; Sachse, Frank B.</creator><creatorcontrib>McNary, Thomas G. ; Bridge, John H.B. ; Sachse, Frank B.</creatorcontrib><description>The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels. Here, we investigated mechanical modulation of the t-system. By applying fluorescent markers, three-dimensional scanning confocal microscopy, and methods of digital image analysis, we studied isolated ventricular cardiomyocytes under different strains. We demonstrate that strain at the cellular level is transmitted to the t-system, reducing the length and volume of tubules and altering their cross-sectional shape. Our data suggest that a cellular strain of as little as 5% affects the shape of transverse tubules, which has important implications for the function of mechanosensitive ion channels found in them. Furthermore, our study supports a prior hypothesis that strain can cause fluid exchange between the t-system and extracellular space.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/j.bpj.2011.03.046</identifier><identifier>PMID: 21575564</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biophysical Letter ; Biophysics ; Cardiomyocytes ; Cell Surface Extensions - metabolism ; extracellular space ; fluorescence ; Heart Ventricles - cytology ; image analysis ; Image Processing, Computer-Assisted ; ion channels ; mammals ; Membranes ; Microscopy ; Microscopy, Confocal ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Proteins ; Rabbits ; Stress, Mechanical</subject><ispartof>Biophysical journal, 2011-05, Vol.100 (10), p.L53-L55</ispartof><rights>2011 Biophysical Society</rights><rights>Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.</rights><rights>Copyright Biophysical Society May 18, 2011</rights><rights>2011 by the Biophysical Society. 2011 Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-37d5f51ecc4dba57061ec5c24a4adb607847e93ccc50ea1f5a45485ce0d9d2673</citedby><cites>FETCH-LOGICAL-c501t-37d5f51ecc4dba57061ec5c24a4adb607847e93ccc50ea1f5a45485ce0d9d2673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093556/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bpj.2011.03.046$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3550,27924,27925,45995,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21575564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McNary, Thomas G.</creatorcontrib><creatorcontrib>Bridge, John H.B.</creatorcontrib><creatorcontrib>Sachse, Frank B.</creatorcontrib><title>Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels. Here, we investigated mechanical modulation of the t-system. By applying fluorescent markers, three-dimensional scanning confocal microscopy, and methods of digital image analysis, we studied isolated ventricular cardiomyocytes under different strains. We demonstrate that strain at the cellular level is transmitted to the t-system, reducing the length and volume of tubules and altering their cross-sectional shape. Our data suggest that a cellular strain of as little as 5% affects the shape of transverse tubules, which has important implications for the function of mechanosensitive ion channels found in them. Furthermore, our study supports a prior hypothesis that strain can cause fluid exchange between the t-system and extracellular space.</description><subject>Animals</subject><subject>Biophysical Letter</subject><subject>Biophysics</subject><subject>Cardiomyocytes</subject><subject>Cell Surface Extensions - metabolism</subject><subject>extracellular space</subject><subject>fluorescence</subject><subject>Heart Ventricles - cytology</subject><subject>image analysis</subject><subject>Image Processing, Computer-Assisted</subject><subject>ion channels</subject><subject>mammals</subject><subject>Membranes</subject><subject>Microscopy</subject><subject>Microscopy, Confocal</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Stress, Mechanical</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV-L1DAUxYso7rj6AXzR4ItPrTdt0rQIggz-gxXBmfU1pOntbEonmU3agX57U7su6oNPuZDfPdxzTpI8p5BRoOWbPmtOfZYDpRkUGbDyQbKhnOUpQFU-TDYAUKYFq_lF8iSEHoDmHOjj5CKnXHBesk0y70avjCV7r2zo0JM4_0A7eqOnQXmyVb417jg7PY8YyOjI_gaNX_kz-oBkPzW_0N0cRjyS73hGNWBLmpnstLLW2APZOts5rQby1Wjvgnan-WnyqFNDwGd372Vy_fHDfvs5vfr26cv2_VWq461jWoiWd5yi1qxtFBdQxpnrnCmm2qYEUTGBdaF1xFHRjivGWcU1Qlu3eSmKy-TdqnuamiO2ejGnBnny5qj8LJ0y8u8fa27kwZ1lAXURQ4oCr-8EvLudMIzyaILGYVAW3RRkVQomWCkW8tU_ZO8mb6M7WQkKRZ3XLEJ0hZYggsfu_hQKcqlV9jLWKpdaJRQy1hp3Xvzp4X7jd48ReLkCnXJSHbwJ8noXFXjsnNIKFom3K4Ex67NBL4M2aDW2xqMeZevMfw74CadUv0E</recordid><startdate>20110518</startdate><enddate>20110518</enddate><creator>McNary, Thomas G.</creator><creator>Bridge, John H.B.</creator><creator>Sachse, Frank B.</creator><general>Elsevier Inc</general><general>Biophysical Society</general><general>The Biophysical Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110518</creationdate><title>Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy</title><author>McNary, Thomas G. ; Bridge, John H.B. ; Sachse, Frank B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-37d5f51ecc4dba57061ec5c24a4adb607847e93ccc50ea1f5a45485ce0d9d2673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biophysical Letter</topic><topic>Biophysics</topic><topic>Cardiomyocytes</topic><topic>Cell Surface Extensions - metabolism</topic><topic>extracellular space</topic><topic>fluorescence</topic><topic>Heart Ventricles - cytology</topic><topic>image analysis</topic><topic>Image Processing, Computer-Assisted</topic><topic>ion channels</topic><topic>mammals</topic><topic>Membranes</topic><topic>Microscopy</topic><topic>Microscopy, Confocal</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McNary, Thomas G.</creatorcontrib><creatorcontrib>Bridge, John H.B.</creatorcontrib><creatorcontrib>Sachse, Frank B.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McNary, Thomas G.</au><au>Bridge, John H.B.</au><au>Sachse, Frank B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>2011-05-18</date><risdate>2011</risdate><volume>100</volume><issue>10</issue><spage>L53</spage><epage>L55</epage><pages>L53-L55</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels. Here, we investigated mechanical modulation of the t-system. By applying fluorescent markers, three-dimensional scanning confocal microscopy, and methods of digital image analysis, we studied isolated ventricular cardiomyocytes under different strains. We demonstrate that strain at the cellular level is transmitted to the t-system, reducing the length and volume of tubules and altering their cross-sectional shape. Our data suggest that a cellular strain of as little as 5% affects the shape of transverse tubules, which has important implications for the function of mechanosensitive ion channels found in them. Furthermore, our study supports a prior hypothesis that strain can cause fluid exchange between the t-system and extracellular space.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21575564</pmid><doi>10.1016/j.bpj.2011.03.046</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3495 |
| ispartof | Biophysical journal, 2011-05, Vol.100 (10), p.L53-L55 |
| issn | 0006-3495 1542-0086 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3093556 |
| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Biophysical Letter Biophysics Cardiomyocytes Cell Surface Extensions - metabolism extracellular space fluorescence Heart Ventricles - cytology image analysis Image Processing, Computer-Assisted ion channels mammals Membranes Microscopy Microscopy, Confocal Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Proteins Rabbits Stress, Mechanical |
| title | Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T02%3A02%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Strain%20Transfer%20in%20Ventricular%20Cardiomyocytes%20to%20Their%20Transverse%20Tubular%20System%20Revealed%20by%20Scanning%20Confocal%20Microscopy&rft.jtitle=Biophysical%20journal&rft.au=McNary,%20Thomas%C2%A0G.&rft.date=2011-05-18&rft.volume=100&rft.issue=10&rft.spage=L53&rft.epage=L55&rft.pages=L53-L55&rft.issn=0006-3495&rft.eissn=1542-0086&rft_id=info:doi/10.1016/j.bpj.2011.03.046&rft_dat=%3Cproquest_pubme%3E867474676%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=871039294&rft_id=info:pmid/21575564&rft_els_id=S0006349511004048&rfr_iscdi=true |