Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry

Large, fluorescently stained restriction fragments of lambda phage DNA are sized by passing Individual fragments through a focused continuous wave laser beam In an ultrasensitive flow cytometer at a rate of 60 fragments per second. The size of the fluorescence burst emitted by each stained DNA fragm...

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Veröffentlicht in:	Nucleic acids research 1993-02, Vol.21 (4), p.803-806
Hauptverfasser:	Goodwin, Peter M., Johnson, Mitchell E., Martin, John C., Ambrose, W.Patrick, Marrone, Babetta L., Jett, James H., Keller, Richard A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Bacteriophage lambda Biological and medical sciences Deoxyribonucleases, Type II Site-Specific DNA - chemistry DNA, Viral - chemistry Flow Cytometry - methods Fluorescent Dyes Fundamental and applied biological sciences. Psychology General aspects, investigation methods Lasers Nucleic acids phage lambda
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