Downstream targets of GATA3 in the vestibular sensory organs of the inner ear
Haploinsufficiency for the transcription factor GATA3 leads to hearing loss in humans. It is expressed throughout the auditory sensory epithelium (SE). In the vestibular organs, GATA3 is limited to the striola reversal zone of the utricle. Stereocilia orientation shifts 180° at this region, which co...
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| Veröffentlicht in: | Developmental dynamics 2009-12, Vol.238 (12), p.3093-3102 |
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| creator | Alvarado, David M. Veile, Rose Speck, Judith Warchol, Mark Lovett, Michael |
| description | Haploinsufficiency for the transcription factor GATA3 leads to hearing loss in humans. It is expressed throughout the auditory sensory epithelium (SE). In the vestibular organs, GATA3 is limited to the striola reversal zone of the utricle. Stereocilia orientation shifts 180° at this region, which contains morphologically distinct type‐I hair cells. The striola is conserved in all amniotes, its function is unknown, and GATA3 is the only known marker of the reversal zone. To identify downstream targets of GATA3 that might point to striolar function, we measured gene expression differences between striolar and extra‐striolar SE. These were compared with profiles after GATA3 RNAi and GATA3 over‐expression. We identified four genes (BMP2, FKHL18, LMO4, and MBNL2) that consistently varied with GATA3. Two of these (LMO4 and MBNL2) were shown to be direct targets of GATA3 by ChIP. Our results suggest that GATA3 impacts WNT signaling in this region of the sensory macula. Developmental Dynamics 238:3093–3102, 2009. © 2009 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/dvdy.22149 |
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It is expressed throughout the auditory sensory epithelium (SE). In the vestibular organs, GATA3 is limited to the striola reversal zone of the utricle. Stereocilia orientation shifts 180° at this region, which contains morphologically distinct type‐I hair cells. The striola is conserved in all amniotes, its function is unknown, and GATA3 is the only known marker of the reversal zone. To identify downstream targets of GATA3 that might point to striolar function, we measured gene expression differences between striolar and extra‐striolar SE. These were compared with profiles after GATA3 RNAi and GATA3 over‐expression. We identified four genes (BMP2, FKHL18, LMO4, and MBNL2) that consistently varied with GATA3. Two of these (LMO4 and MBNL2) were shown to be direct targets of GATA3 by ChIP. Our results suggest that GATA3 impacts WNT signaling in this region of the sensory macula. Developmental Dynamics 238:3093–3102, 2009. © 2009 Wiley‐Liss, Inc.</description><identifier>ISSN: 1058-8388</identifier><identifier>EISSN: 1097-0177</identifier><identifier>DOI: 10.1002/dvdy.22149</identifier><identifier>PMID: 19924793</identifier><language>eng</language><publisher>New York: Wiley‐Liss, Inc</publisher><subject>Animals ; Cells, Cultured ; Chickens ; Ear, Inner - metabolism ; GATA3 Transcription Factor - genetics ; GATA3 Transcription Factor - metabolism ; GATA3 Transcription Factor - physiology ; Gene Expression Profiling ; Gene Expression Regulation ; Hair Cells, Vestibular - metabolism ; inner ear ; Oligonucleotide Array Sequence Analysis ; Saccule and Utricle - metabolism ; transcription factors ; Transfection ; utricle ; Vestibule, Labyrinth - growth & development ; Vestibule, Labyrinth - metabolism ; Wnt Proteins - genetics ; Wnt Proteins - physiology</subject><ispartof>Developmental dynamics, 2009-12, Vol.238 (12), p.3093-3102</ispartof><rights>Copyright © 2009 Wiley‐Liss, Inc.</rights><rights>(c) 2009 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4199-8e53a1ee483ecc6f8303411e09fed4a279dbae433f40aa1b69bdc2b2effd6ac63</citedby><cites>FETCH-LOGICAL-c4199-8e53a1ee483ecc6f8303411e09fed4a279dbae433f40aa1b69bdc2b2effd6ac63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvdy.22149$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvdy.22149$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19924793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alvarado, David M.</creatorcontrib><creatorcontrib>Veile, Rose</creatorcontrib><creatorcontrib>Speck, Judith</creatorcontrib><creatorcontrib>Warchol, Mark</creatorcontrib><creatorcontrib>Lovett, Michael</creatorcontrib><title>Downstream targets of GATA3 in the vestibular sensory organs of the inner ear</title><title>Developmental dynamics</title><addtitle>Dev Dyn</addtitle><description>Haploinsufficiency for the transcription factor GATA3 leads to hearing loss in humans. It is expressed throughout the auditory sensory epithelium (SE). In the vestibular organs, GATA3 is limited to the striola reversal zone of the utricle. Stereocilia orientation shifts 180° at this region, which contains morphologically distinct type‐I hair cells. The striola is conserved in all amniotes, its function is unknown, and GATA3 is the only known marker of the reversal zone. To identify downstream targets of GATA3 that might point to striolar function, we measured gene expression differences between striolar and extra‐striolar SE. These were compared with profiles after GATA3 RNAi and GATA3 over‐expression. We identified four genes (BMP2, FKHL18, LMO4, and MBNL2) that consistently varied with GATA3. Two of these (LMO4 and MBNL2) were shown to be direct targets of GATA3 by ChIP. Our results suggest that GATA3 impacts WNT signaling in this region of the sensory macula. Developmental Dynamics 238:3093–3102, 2009. © 2009 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>Ear, Inner - metabolism</subject><subject>GATA3 Transcription Factor - genetics</subject><subject>GATA3 Transcription Factor - metabolism</subject><subject>GATA3 Transcription Factor - physiology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Hair Cells, Vestibular - metabolism</subject><subject>inner ear</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Saccule and Utricle - metabolism</subject><subject>transcription factors</subject><subject>Transfection</subject><subject>utricle</subject><subject>Vestibule, Labyrinth - growth & development</subject><subject>Vestibule, Labyrinth - metabolism</subject><subject>Wnt Proteins - genetics</subject><subject>Wnt Proteins - physiology</subject><issn>1058-8388</issn><issn>1097-0177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMobk5v_AGSO0HoTJqsbW6EsekUJt5MwauQtqdbpUtm0m7039uuw48br3IgD897zovQJSVDSoh_m27Teuj7lIsj1KdEhB6hYXjczqPIi1gU9dCZcx-EkCjg9BT1qBA-DwXro-ep2WlXWlBrXCq7hNJhk-HZeDFmONe4XAHegivzuCqUxQ60M7bGxi6V3pMtkGsNFoOy5-gkU4WDi8M7QK8P94vJozd_mT1NxnMv4U20F8GIKQrAIwZJEmQRI4xTCkRkkHLlhyKNFXDGMk6UonEg4jTxYx-yLA1UErABuuu8mypeQ5qALq0q5Mbma2VraVQu__7ofCWXZitZ2wDhjeD6ILDms2ruk-vcJVAUSoOpnAwZC4kvRBt105GJNc5ZyL5TKJFt_bKtX-7rb-Cr33v9oIe-G4B2wC4voP5HJadv0_dO-gUbTZJF</recordid><startdate>200912</startdate><enddate>200912</enddate><creator>Alvarado, David M.</creator><creator>Veile, Rose</creator><creator>Speck, Judith</creator><creator>Warchol, Mark</creator><creator>Lovett, Michael</creator><general>Wiley‐Liss, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200912</creationdate><title>Downstream targets of GATA3 in the vestibular sensory organs of the inner ear</title><author>Alvarado, David M. ; Veile, Rose ; Speck, Judith ; Warchol, Mark ; Lovett, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4199-8e53a1ee483ecc6f8303411e09fed4a279dbae433f40aa1b69bdc2b2effd6ac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Ear, Inner - metabolism</topic><topic>GATA3 Transcription Factor - genetics</topic><topic>GATA3 Transcription Factor - metabolism</topic><topic>GATA3 Transcription Factor - physiology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Hair Cells, Vestibular - metabolism</topic><topic>inner ear</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Saccule and Utricle - metabolism</topic><topic>transcription factors</topic><topic>Transfection</topic><topic>utricle</topic><topic>Vestibule, Labyrinth - growth & development</topic><topic>Vestibule, Labyrinth - metabolism</topic><topic>Wnt Proteins - genetics</topic><topic>Wnt Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alvarado, David M.</creatorcontrib><creatorcontrib>Veile, Rose</creatorcontrib><creatorcontrib>Speck, Judith</creatorcontrib><creatorcontrib>Warchol, Mark</creatorcontrib><creatorcontrib>Lovett, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Developmental dynamics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alvarado, David M.</au><au>Veile, Rose</au><au>Speck, Judith</au><au>Warchol, Mark</au><au>Lovett, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downstream targets of GATA3 in the vestibular sensory organs of the inner ear</atitle><jtitle>Developmental dynamics</jtitle><addtitle>Dev Dyn</addtitle><date>2009-12</date><risdate>2009</risdate><volume>238</volume><issue>12</issue><spage>3093</spage><epage>3102</epage><pages>3093-3102</pages><issn>1058-8388</issn><eissn>1097-0177</eissn><abstract>Haploinsufficiency for the transcription factor GATA3 leads to hearing loss in humans. It is expressed throughout the auditory sensory epithelium (SE). In the vestibular organs, GATA3 is limited to the striola reversal zone of the utricle. Stereocilia orientation shifts 180° at this region, which contains morphologically distinct type‐I hair cells. The striola is conserved in all amniotes, its function is unknown, and GATA3 is the only known marker of the reversal zone. To identify downstream targets of GATA3 that might point to striolar function, we measured gene expression differences between striolar and extra‐striolar SE. These were compared with profiles after GATA3 RNAi and GATA3 over‐expression. We identified four genes (BMP2, FKHL18, LMO4, and MBNL2) that consistently varied with GATA3. Two of these (LMO4 and MBNL2) were shown to be direct targets of GATA3 by ChIP. Our results suggest that GATA3 impacts WNT signaling in this region of the sensory macula. Developmental Dynamics 238:3093–3102, 2009. © 2009 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley‐Liss, Inc</pub><pmid>19924793</pmid><doi>10.1002/dvdy.22149</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Cells, Cultured Chickens Ear, Inner - metabolism GATA3 Transcription Factor - genetics GATA3 Transcription Factor - metabolism GATA3 Transcription Factor - physiology Gene Expression Profiling Gene Expression Regulation Hair Cells, Vestibular - metabolism inner ear Oligonucleotide Array Sequence Analysis Saccule and Utricle - metabolism transcription factors Transfection utricle Vestibule, Labyrinth - growth & development Vestibule, Labyrinth - metabolism Wnt Proteins - genetics Wnt Proteins - physiology |
| title | Downstream targets of GATA3 in the vestibular sensory organs of the inner ear |
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