Stability of DNA-Tethered Lipid Membranes with Mobile Tethers
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe, R. D.; Chan, Y−H. M.; Ganesan, P. V.; Boxer, S. G. J. Struct. Biol. 2009, 168, 190−9). When mobile...
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| Veröffentlicht in: | Langmuir 2011-05, Vol.27 (9), p.5492-5497 |
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| description | We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe, R. D.; Chan, Y−H. M.; Ganesan, P. V.; Boxer, S. G. J. Struct. Biol. 2009, 168, 190−9). When mobile DNA tethers are used, the tethered bilayer patches become unstable, while they are stable if the tethers are fixed on the surface. Because the mobile tethers between a patch and a supported lipid bilayer offer a particularly interesting architecture for studying the dynamics of membrane−membrane interactions, we have investigated the sources of instability, focusing on membrane composition. The most stable patches were made with a mixture of saturated lipids and cholesterol, suggesting an important role for membrane stiffness. Other factors such as the effect of tether length, lateral mobility, and patch membrane edge were also investigated. On the basis of these results, a model for the mechanism of patch destruction is developed. |
| doi_str_mv | 10.1021/la200234h |
| format | Article |
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D.; Chan, Y−H. M.; Ganesan, P. V.; Boxer, S. G. J. Struct. Biol. 2009, 168, 190−9). When mobile DNA tethers are used, the tethered bilayer patches become unstable, while they are stable if the tethers are fixed on the surface. Because the mobile tethers between a patch and a supported lipid bilayer offer a particularly interesting architecture for studying the dynamics of membrane−membrane interactions, we have investigated the sources of instability, focusing on membrane composition. The most stable patches were made with a mixture of saturated lipids and cholesterol, suggesting an important role for membrane stiffness. Other factors such as the effect of tether length, lateral mobility, and patch membrane edge were also investigated. On the basis of these results, a model for the mechanism of patch destruction is developed.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/la200234h</identifier><identifier>PMID: 21452847</identifier><identifier>CODEN: LANGD5</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials ; Cell Membrane - chemistry ; Chemistry ; Colloidal state and disperse state ; Coloring Agents - chemistry ; DNA - chemistry ; Exact sciences and technology ; General and physical chemistry ; Lipid Bilayers - chemistry ; Membranes ; Surface Properties ; Unilamellar Liposomes - chemistry</subject><ispartof>Langmuir, 2011-05, Vol.27 (9), p.5492-5497</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-92aaa35bf69a52d9671059cbe5f8f9828c0ac768dbebd76e6b7c659e3b984a43</citedby><cites>FETCH-LOGICAL-a434t-92aaa35bf69a52d9671059cbe5f8f9828c0ac768dbebd76e6b7c659e3b984a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/la200234h$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/la200234h$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24117273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21452847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chung, Minsub</creatorcontrib><creatorcontrib>Boxer, Steven G</creatorcontrib><title>Stability of DNA-Tethered Lipid Membranes with Mobile Tethers</title><title>Langmuir</title><addtitle>Langmuir</addtitle><description>We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe, R. 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On the basis of these results, a model for the mechanism of patch destruction is developed.</description><subject>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials</subject><subject>Cell Membrane - chemistry</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Coloring Agents - chemistry</subject><subject>DNA - chemistry</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Lipid Bilayers - chemistry</subject><subject>Membranes</subject><subject>Surface Properties</subject><subject>Unilamellar Liposomes - chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtKAzEUBuAgiq2XhS8gsxFxMZr7ZaFQvEOrC7sPSSZjI9NOTaaKb2-ktSq4yuJ8_OfkB-AAwVMEMTprDIYQEzrZAH3EMCyZxGIT9KGgpBSUkx7YSekFQqgIVdughxFlWFLRB-dPnbGhCd1H0dbF1cOgHPtu4qOvimGYh6oY-amNZuZT8R66STFqs_bFEqU9sFWbJvn91bsLxjfX48u7cvh4e385GJaGEtqVChtjCLM1V4bhSnGBIFPOelbLWkksHTROcFlZbyvBPbfCcaY8sUrSHLELLpax84Wd-sr5WRdNo-cxTE380K0J-u9kFib6uX3TBEoGEckBx6uA2L4ufOr0NCTnmyZ_rF0kLTkRXDAqsjxZShfblKKv11sQ1F9l63XZ2R7-Pmstv9vN4GgFTHKmqXOPLqQfRxESWJAfZ1zSL-0iznKZ_yz8BI5Ykuo</recordid><startdate>20110503</startdate><enddate>20110503</enddate><creator>Chung, Minsub</creator><creator>Boxer, Steven G</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110503</creationdate><title>Stability of DNA-Tethered Lipid Membranes with Mobile Tethers</title><author>Chung, Minsub ; Boxer, Steven G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-92aaa35bf69a52d9671059cbe5f8f9828c0ac768dbebd76e6b7c659e3b984a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials</topic><topic>Cell Membrane - chemistry</topic><topic>Chemistry</topic><topic>Colloidal state and disperse state</topic><topic>Coloring Agents - chemistry</topic><topic>DNA - chemistry</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Lipid Bilayers - chemistry</topic><topic>Membranes</topic><topic>Surface Properties</topic><topic>Unilamellar Liposomes - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chung, Minsub</creatorcontrib><creatorcontrib>Boxer, Steven G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chung, Minsub</au><au>Boxer, Steven G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability of DNA-Tethered Lipid Membranes with Mobile Tethers</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2011-05-03</date><risdate>2011</risdate><volume>27</volume><issue>9</issue><spage>5492</spage><epage>5497</epage><pages>5492-5497</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe, R. D.; Chan, Y−H. M.; Ganesan, P. V.; Boxer, S. G. J. Struct. Biol. 2009, 168, 190−9). When mobile DNA tethers are used, the tethered bilayer patches become unstable, while they are stable if the tethers are fixed on the surface. Because the mobile tethers between a patch and a supported lipid bilayer offer a particularly interesting architecture for studying the dynamics of membrane−membrane interactions, we have investigated the sources of instability, focusing on membrane composition. The most stable patches were made with a mixture of saturated lipids and cholesterol, suggesting an important role for membrane stiffness. Other factors such as the effect of tether length, lateral mobility, and patch membrane edge were also investigated. 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| source | ACS Publications; MEDLINE |
| subjects | Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials Cell Membrane - chemistry Chemistry Colloidal state and disperse state Coloring Agents - chemistry DNA - chemistry Exact sciences and technology General and physical chemistry Lipid Bilayers - chemistry Membranes Surface Properties Unilamellar Liposomes - chemistry |
| title | Stability of DNA-Tethered Lipid Membranes with Mobile Tethers |
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