Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice
This study evaluates the influence of particle size, PEGylation, and surface coating on the quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) in mice. Polymer‐ or peptide‐coated 64Cu‐labeled QDs 2 or 12 nm in diameter, with or without polyethylene glycol (PEG) of molecular we...
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| Veröffentlicht in: | Small (Weinheim an der Bergstrasse, Germany) Germany), 2009-01, Vol.5 (1), p.126-134 |
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| creator | Schipper, Meike L. Iyer, Gopal Koh, Ai Leen Cheng, Zhen Ebenstein, Yuval Aharoni, Assaf Keren, Shay Bentolila, Laurent A. Li, Jianquing Rao, Jianghong Chen, Xiaoyuan Banin, Uri Wu, Anna M. Sinclair, Robert Weiss, Shimon Gambhir, Sanjiv S. |
| description | This study evaluates the influence of particle size, PEGylation, and surface coating on the quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) in mice. Polymer‐ or peptide‐coated 64Cu‐labeled QDs 2 or 12 nm in diameter, with or without polyethylene glycol (PEG) of molecular weight 2000, are studied by serial micropositron emission tomography imaging and region‐of‐interest analysis, as well as transmission electron microscopy and inductively coupled plasma mass spectrometry. PEGylation and peptide coating slow QD uptake into the organs of the reticuloendothelial system (RES), liver and spleen, by a factor of 6–9 and 2–3, respectively. Small particles are in part renally excreted. Peptide‐coated particles are cleared from liver faster than physical decay alone would suggest. Renal excretion of small QDs and slowing of RES clearance by PEGylation or peptide surface coating are encouraging steps toward the use of modified QDs for imaging living subjects.
The living image: Quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) is studied in mice by micro positron emission tomography. PEGylation (PEG = polyethylene glycol) and peptide coating slow QD uptake into liver, spleen, and bone. Small peptide‐coated QDs are in part renally excreted (see picture; < indicates bladder uptake). |
| doi_str_mv | 10.1002/smll.200800003 |
| format | Article |
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The living image: Quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) is studied in mice by micro positron emission tomography. PEGylation (PEG = polyethylene glycol) and peptide coating slow QD uptake into liver, spleen, and bone. Small peptide‐coated QDs are in part renally excreted (see picture; < indicates bladder uptake).</description><identifier>ISSN: 1613-6810</identifier><identifier>EISSN: 1613-6829</identifier><identifier>DOI: 10.1002/smll.200800003</identifier><identifier>PMID: 19051182</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Animals ; biodistribution ; imaging ; Liver - metabolism ; Mice ; Microscopy, Electron, Transmission ; Molecular Weight ; nanoparticles ; Particle Size ; Peptides - chemistry ; Polyethylene Glycols - chemistry ; Positron-Emission Tomography ; Quantum Dots ; Spleen - metabolism ; Surface Properties ; tomography</subject><ispartof>Small (Weinheim an der Bergstrasse, Germany), 2009-01, Vol.5 (1), p.126-134</ispartof><rights>Copyright © 2009 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5733-fb7d63bfc167e9d5307cb52f02189b7a4f824e844c94d21e34ff941f3db54333</citedby><cites>FETCH-LOGICAL-c5733-fb7d63bfc167e9d5307cb52f02189b7a4f824e844c94d21e34ff941f3db54333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsmll.200800003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsmll.200800003$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19051182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schipper, Meike L.</creatorcontrib><creatorcontrib>Iyer, Gopal</creatorcontrib><creatorcontrib>Koh, Ai Leen</creatorcontrib><creatorcontrib>Cheng, Zhen</creatorcontrib><creatorcontrib>Ebenstein, Yuval</creatorcontrib><creatorcontrib>Aharoni, Assaf</creatorcontrib><creatorcontrib>Keren, Shay</creatorcontrib><creatorcontrib>Bentolila, Laurent A.</creatorcontrib><creatorcontrib>Li, Jianquing</creatorcontrib><creatorcontrib>Rao, Jianghong</creatorcontrib><creatorcontrib>Chen, Xiaoyuan</creatorcontrib><creatorcontrib>Banin, Uri</creatorcontrib><creatorcontrib>Wu, Anna M.</creatorcontrib><creatorcontrib>Sinclair, Robert</creatorcontrib><creatorcontrib>Weiss, Shimon</creatorcontrib><creatorcontrib>Gambhir, Sanjiv S.</creatorcontrib><title>Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice</title><title>Small (Weinheim an der Bergstrasse, Germany)</title><addtitle>Small</addtitle><description>This study evaluates the influence of particle size, PEGylation, and surface coating on the quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) in mice. Polymer‐ or peptide‐coated 64Cu‐labeled QDs 2 or 12 nm in diameter, with or without polyethylene glycol (PEG) of molecular weight 2000, are studied by serial micropositron emission tomography imaging and region‐of‐interest analysis, as well as transmission electron microscopy and inductively coupled plasma mass spectrometry. PEGylation and peptide coating slow QD uptake into the organs of the reticuloendothelial system (RES), liver and spleen, by a factor of 6–9 and 2–3, respectively. Small particles are in part renally excreted. Peptide‐coated particles are cleared from liver faster than physical decay alone would suggest. Renal excretion of small QDs and slowing of RES clearance by PEGylation or peptide surface coating are encouraging steps toward the use of modified QDs for imaging living subjects.
The living image: Quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) is studied in mice by micro positron emission tomography. PEGylation (PEG = polyethylene glycol) and peptide coating slow QD uptake into liver, spleen, and bone. Small peptide‐coated QDs are in part renally excreted (see picture; < indicates bladder uptake).</description><subject>Animals</subject><subject>biodistribution</subject><subject>imaging</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Weight</subject><subject>nanoparticles</subject><subject>Particle Size</subject><subject>Peptides - chemistry</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Positron-Emission Tomography</subject><subject>Quantum Dots</subject><subject>Spleen - metabolism</subject><subject>Surface Properties</subject><subject>tomography</subject><issn>1613-6810</issn><issn>1613-6829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAUxyMEYmNw5Yh84rQU_0rsXJBY2cqkbBS1EkfLcezNzLGHnYyVvx5vrco44Yv99D7v8yx9i-ItgjMEIf6QBudmGEIO8yHPikNUI1LWHDfP928ED4pXKf3IAMKUvSwOUAMrhDg-LG6WMo5WOQ1W9rc-BqspGqk0mAc5Wn91DKTvwfJ0sXG5Dh6ce-Mm7TMxXmtwYkNv0xhtNz12gwHfJunHaQCfw5iA9aC1d9kDLqzSr4sXRrqk3-zuo2J9drqefynbr4vz-ae2VBUjpDQd62vSGYVqppu-IpCprsIGYsSbjklqOKaaU6oa2mOkCTWmociQvqsoIeSo-LjV3k7doHul_RilE7fRDjJuRJBW_Nvx9lpchTtBIKd11WTB-50ghp-TTqMYbFLaOel1mJIgmNewetw024IqhpSiNvslCIqHeMRDPGIfTx549_Rrf_FdHhlotsAv6_TmPzqxumjbp_JyO5sT0ff7WRlvRM0Iq8T3y4XgJ6vL5XLNRE3-AORfrQo</recordid><startdate>200901</startdate><enddate>200901</enddate><creator>Schipper, Meike L.</creator><creator>Iyer, Gopal</creator><creator>Koh, Ai Leen</creator><creator>Cheng, Zhen</creator><creator>Ebenstein, Yuval</creator><creator>Aharoni, Assaf</creator><creator>Keren, Shay</creator><creator>Bentolila, Laurent A.</creator><creator>Li, Jianquing</creator><creator>Rao, Jianghong</creator><creator>Chen, Xiaoyuan</creator><creator>Banin, Uri</creator><creator>Wu, Anna M.</creator><creator>Sinclair, Robert</creator><creator>Weiss, Shimon</creator><creator>Gambhir, Sanjiv S.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>200901</creationdate><title>Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice</title><author>Schipper, Meike L. ; Iyer, Gopal ; Koh, Ai Leen ; Cheng, Zhen ; Ebenstein, Yuval ; Aharoni, Assaf ; Keren, Shay ; Bentolila, Laurent A. ; Li, Jianquing ; Rao, Jianghong ; Chen, Xiaoyuan ; Banin, Uri ; Wu, Anna M. ; Sinclair, Robert ; Weiss, Shimon ; Gambhir, Sanjiv S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5733-fb7d63bfc167e9d5307cb52f02189b7a4f824e844c94d21e34ff941f3db54333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>biodistribution</topic><topic>imaging</topic><topic>Liver - metabolism</topic><topic>Mice</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Weight</topic><topic>nanoparticles</topic><topic>Particle Size</topic><topic>Peptides - chemistry</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Positron-Emission Tomography</topic><topic>Quantum Dots</topic><topic>Spleen - metabolism</topic><topic>Surface Properties</topic><topic>tomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schipper, Meike L.</creatorcontrib><creatorcontrib>Iyer, Gopal</creatorcontrib><creatorcontrib>Koh, Ai Leen</creatorcontrib><creatorcontrib>Cheng, Zhen</creatorcontrib><creatorcontrib>Ebenstein, Yuval</creatorcontrib><creatorcontrib>Aharoni, Assaf</creatorcontrib><creatorcontrib>Keren, Shay</creatorcontrib><creatorcontrib>Bentolila, Laurent A.</creatorcontrib><creatorcontrib>Li, Jianquing</creatorcontrib><creatorcontrib>Rao, Jianghong</creatorcontrib><creatorcontrib>Chen, Xiaoyuan</creatorcontrib><creatorcontrib>Banin, Uri</creatorcontrib><creatorcontrib>Wu, Anna M.</creatorcontrib><creatorcontrib>Sinclair, Robert</creatorcontrib><creatorcontrib>Weiss, Shimon</creatorcontrib><creatorcontrib>Gambhir, Sanjiv S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schipper, Meike L.</au><au>Iyer, Gopal</au><au>Koh, Ai Leen</au><au>Cheng, Zhen</au><au>Ebenstein, Yuval</au><au>Aharoni, Assaf</au><au>Keren, Shay</au><au>Bentolila, Laurent A.</au><au>Li, Jianquing</au><au>Rao, Jianghong</au><au>Chen, Xiaoyuan</au><au>Banin, Uri</au><au>Wu, Anna M.</au><au>Sinclair, Robert</au><au>Weiss, Shimon</au><au>Gambhir, Sanjiv S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice</atitle><jtitle>Small (Weinheim an der Bergstrasse, Germany)</jtitle><addtitle>Small</addtitle><date>2009-01</date><risdate>2009</risdate><volume>5</volume><issue>1</issue><spage>126</spage><epage>134</epage><pages>126-134</pages><issn>1613-6810</issn><eissn>1613-6829</eissn><abstract>This study evaluates the influence of particle size, PEGylation, and surface coating on the quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) in mice. Polymer‐ or peptide‐coated 64Cu‐labeled QDs 2 or 12 nm in diameter, with or without polyethylene glycol (PEG) of molecular weight 2000, are studied by serial micropositron emission tomography imaging and region‐of‐interest analysis, as well as transmission electron microscopy and inductively coupled plasma mass spectrometry. PEGylation and peptide coating slow QD uptake into the organs of the reticuloendothelial system (RES), liver and spleen, by a factor of 6–9 and 2–3, respectively. Small particles are in part renally excreted. Peptide‐coated particles are cleared from liver faster than physical decay alone would suggest. Renal excretion of small QDs and slowing of RES clearance by PEGylation or peptide surface coating are encouraging steps toward the use of modified QDs for imaging living subjects.
The living image: Quantitative biodistribution of near‐infrared‐emitting quantum dots (QDs) is studied in mice by micro positron emission tomography. PEGylation (PEG = polyethylene glycol) and peptide coating slow QD uptake into liver, spleen, and bone. Small peptide‐coated QDs are in part renally excreted (see picture; < indicates bladder uptake).</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>19051182</pmid><doi>10.1002/smll.200800003</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals biodistribution imaging Liver - metabolism Mice Microscopy, Electron, Transmission Molecular Weight nanoparticles Particle Size Peptides - chemistry Polyethylene Glycols - chemistry Positron-Emission Tomography Quantum Dots Spleen - metabolism Surface Properties tomography |
| title | Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice |
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