The expression of αA- and βB1-crystallin during normal development and regeneration, and proteomic analysis for the regenerating lens in Xenopus laevis

To explore the expression of the lens crystallins (αA- and βB1-crystallin) in Xenopus laevis embryonic lens development and regeneration and to analyze the order of different crystallins generated in the regenerating lens. Real Time-PCR, Immunofluorescence, and 2D-PAGE were used to analyze the expre...

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Veröffentlicht in:	Molecular vision 2011-03, Vol.17, p.768-778
Hauptverfasser:	Zhao, Yongqing, Ju, Furong, Zhao, Yuanlin, Wang, Lei, Sun, Zhenglong, Liu, Mingxin, Gao, Lan
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Crystallin A Chain - genetics alpha-Crystallin A Chain - metabolism Animals beta-Crystallin B Chain - genetics beta-Crystallin B Chain - metabolism Cloning, Molecular Ectoderm - metabolism Electrophoresis, Gel, Two-Dimensional Embryo, Nonmammalian - metabolism Embryonic Development - genetics Escherichia coli Gene Expression Regulation, Developmental In Situ Hybridization Lens, Crystalline - embryology Lens, Crystalline - metabolism Polymerase Chain Reaction Proteomics Recombinant Proteins - genetics Recombinant Proteins - metabolism Regeneration - genetics RNA, Messenger - analysis RNA, Messenger - biosynthesis Xenopus laevis Xenopus laevis - embryology Xenopus laevis - genetics Xenopus laevis - metabolism
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