The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions
Endothelial progenitor cells (EPCs) derived from bone marrow are known to be heterogeneous. In this study, we tried to find favorable conditions that induce the differentiation of mononuclear cells (MNCs) from bone marrow into EPCs. The differentiation capacity of MNCs from rat bone marrow was inves...
Gespeichert in:
| Veröffentlicht in: | Cytotechnology (Dordrecht) 2011-05, Vol.63 (3), p.217-226 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 226 |
|---|---|
| container_issue | 3 |
| container_start_page | 217 |
| container_title | Cytotechnology (Dordrecht) |
| container_volume | 63 |
| creator | Yang, Nana Li, Dawei Jiao, Peng Chen, Bin Yao, Shutong Sang, Hui Yang, Mingfeng Han, Jiju Zhang, Ying Qin, Shucun |
| description | Endothelial progenitor cells (EPCs) derived from bone marrow are known to be heterogeneous. In this study, we tried to find favorable conditions that induce the differentiation of mononuclear cells (MNCs) from bone marrow into EPCs. The differentiation capacity of MNCs from rat bone marrow was investigated in different conditions, such as different media, different induction times and different culture surfaces. The cell morphology and endothelial biomarkers associated with differentiated MNCs were studied. Our results indicated that MNCs cultured in EGM-2MV (Endothelial cell basal medium-2, plus SingleQuots of growth supplements) developed a bursiform shape, a late EPC-like morphology, while MNCs cultured in complete medium (CM, M199 with 10% FBS, 20 ng/mL VEGF and 10 ng/mL bFGF) showed a spindle shape, an early EPC-like morphology. Cells of both morphologies were able to incorporate DiI-ac-LDL and bind lectin in vitro. MNCs cultured in EGM-2MV exhibited a higher proliferation rate and higher eNOS expression than MNCs cultured in CM. MNCs cultured in EGM-2MV had the ability to form tubes on Matrigel. Flow cytometry results indicated that CD133 expression was highest at day 12 and that the greatest number of cells positive for both FLK-1 and CD133 appeared at day 20 from cells cultured in dishes without fibronectin coating. In addition, the expression levels of CD133, CD31 and FLK-1/CD133 were not significantly different between cells of different shapes. Our experiments suggest that MNCs from bone marrow can be differentiated into late EP-like cells in EGM-2MV, which have the ability to rapidly proliferate. These MNCs can also be differentiated into early EP-like cells in CM. Additionally, fibronectin may not be necessary for the differentiation of EPCs to mature ECs after three generations. Differentiated MNCs from bone marrow in EGM-2MV have the characteristics of EPCs, although the expression levels of EPC markers were lower than previously reported. |
| doi_str_mv | 10.1007/s10616-010-9329-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3081048</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>863428120</sourcerecordid><originalsourceid>FETCH-LOGICAL-c565t-34d5397de9f116b89e7056c7a7a317da354abd4d5bd835c35c2811f2e5fb55773</originalsourceid><addsrcrecordid>eNp1kc2KFDEUhYMoTjv6AG4kIOKqND-VSmUjyOAfDLgZ1yGV3HRnqE7aJDXiA_jepuieGRWEQBbnu_eew0HoOSVvKCHybaFkoENHKOkUZ6pjD9CGCsk7IuX4EG2IYk0hgzpDT0q5JoQoSfljdMYo53QQYoN-Xe0A253JxlbIodRgC04eQ3Sp7mAOZsaHnLYQQ00ZW5jngl0jb8Bhn9Me71NMcbEzmFu5jWdT8ZQi4L3JOf3AIWIXvIcMsWK7zHXJ7WyKLtSQYnmKHnkzF3h2-s_Rt48fri4-d5dfP325eH_ZWTGI2vHeCa6kA-UpHaZRgSRisNJIw6l0hoveTK5Bkxu5sO2xkVLPQPhJCCn5OXp33HtYpj0429xkM-tDDs3nT51M0H8rMez0Nt1oTkZK-rEteH1akNP3BUrV-1DW1CZCWooeB963m4w08uU_5HVacmzpNFN0ZENjVooeKZtTKRn8nRdK9NqxPnasW8d67VizNvPizxB3E7elNuDVCTDFmtlnE20o91xP2ajoyrEjV5oUt5DvLf7_-m9mFMID</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918262030</pqid></control><display><type>article</type><title>The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions</title><source>SpringerNature Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>ProQuest Central UK/Ireland</source><source>PubMed Central</source><source>ProQuest Central</source><creator>Yang, Nana ; Li, Dawei ; Jiao, Peng ; Chen, Bin ; Yao, Shutong ; Sang, Hui ; Yang, Mingfeng ; Han, Jiju ; Zhang, Ying ; Qin, Shucun</creator><creatorcontrib>Yang, Nana ; Li, Dawei ; Jiao, Peng ; Chen, Bin ; Yao, Shutong ; Sang, Hui ; Yang, Mingfeng ; Han, Jiju ; Zhang, Ying ; Qin, Shucun</creatorcontrib><description>Endothelial progenitor cells (EPCs) derived from bone marrow are known to be heterogeneous. In this study, we tried to find favorable conditions that induce the differentiation of mononuclear cells (MNCs) from bone marrow into EPCs. The differentiation capacity of MNCs from rat bone marrow was investigated in different conditions, such as different media, different induction times and different culture surfaces. The cell morphology and endothelial biomarkers associated with differentiated MNCs were studied. Our results indicated that MNCs cultured in EGM-2MV (Endothelial cell basal medium-2, plus SingleQuots of growth supplements) developed a bursiform shape, a late EPC-like morphology, while MNCs cultured in complete medium (CM, M199 with 10% FBS, 20 ng/mL VEGF and 10 ng/mL bFGF) showed a spindle shape, an early EPC-like morphology. Cells of both morphologies were able to incorporate DiI-ac-LDL and bind lectin in vitro. MNCs cultured in EGM-2MV exhibited a higher proliferation rate and higher eNOS expression than MNCs cultured in CM. MNCs cultured in EGM-2MV had the ability to form tubes on Matrigel. Flow cytometry results indicated that CD133 expression was highest at day 12 and that the greatest number of cells positive for both FLK-1 and CD133 appeared at day 20 from cells cultured in dishes without fibronectin coating. In addition, the expression levels of CD133, CD31 and FLK-1/CD133 were not significantly different between cells of different shapes. Our experiments suggest that MNCs from bone marrow can be differentiated into late EP-like cells in EGM-2MV, which have the ability to rapidly proliferate. These MNCs can also be differentiated into early EP-like cells in CM. Additionally, fibronectin may not be necessary for the differentiation of EPCs to mature ECs after three generations. Differentiated MNCs from bone marrow in EGM-2MV have the characteristics of EPCs, although the expression levels of EPC markers were lower than previously reported.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-010-9329-2</identifier><identifier>PMID: 21331655</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antibodies ; Antigens ; Biochemistry ; Biological and medical sciences ; Biomarkers ; Biomedicine ; Biotechnology ; Bone marrow ; Cell culture ; Cell cycle ; Cell differentiation ; Cell morphology ; Chemistry ; Chemistry and Materials Science ; Cytology ; Endothelial cells ; Fibroblast growth factor 2 ; Fibronectin ; Flow cytometry ; Fundamental and applied biological sciences. Psychology ; Laboratory animals ; Leukocytes (mononuclear) ; Low density lipoprotein ; Method in Cell Science ; Morphology ; Osteoprogenitor cells ; Progenitor cells ; Vascular endothelial growth factor ; Vascular endothelial growth factor receptor 2</subject><ispartof>Cytotechnology (Dordrecht), 2011-05, Vol.63 (3), p.217-226</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2011.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-34d5397de9f116b89e7056c7a7a317da354abd4d5bd835c35c2811f2e5fb55773</citedby><cites>FETCH-LOGICAL-c565t-34d5397de9f116b89e7056c7a7a317da354abd4d5bd835c35c2811f2e5fb55773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3081048/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918262030?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,27924,27925,33744,33745,41488,42557,43805,51319,53791,53793,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24128915$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21331655$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Nana</creatorcontrib><creatorcontrib>Li, Dawei</creatorcontrib><creatorcontrib>Jiao, Peng</creatorcontrib><creatorcontrib>Chen, Bin</creatorcontrib><creatorcontrib>Yao, Shutong</creatorcontrib><creatorcontrib>Sang, Hui</creatorcontrib><creatorcontrib>Yang, Mingfeng</creatorcontrib><creatorcontrib>Han, Jiju</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Qin, Shucun</creatorcontrib><title>The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Endothelial progenitor cells (EPCs) derived from bone marrow are known to be heterogeneous. In this study, we tried to find favorable conditions that induce the differentiation of mononuclear cells (MNCs) from bone marrow into EPCs. The differentiation capacity of MNCs from rat bone marrow was investigated in different conditions, such as different media, different induction times and different culture surfaces. The cell morphology and endothelial biomarkers associated with differentiated MNCs were studied. Our results indicated that MNCs cultured in EGM-2MV (Endothelial cell basal medium-2, plus SingleQuots of growth supplements) developed a bursiform shape, a late EPC-like morphology, while MNCs cultured in complete medium (CM, M199 with 10% FBS, 20 ng/mL VEGF and 10 ng/mL bFGF) showed a spindle shape, an early EPC-like morphology. Cells of both morphologies were able to incorporate DiI-ac-LDL and bind lectin in vitro. MNCs cultured in EGM-2MV exhibited a higher proliferation rate and higher eNOS expression than MNCs cultured in CM. MNCs cultured in EGM-2MV had the ability to form tubes on Matrigel. Flow cytometry results indicated that CD133 expression was highest at day 12 and that the greatest number of cells positive for both FLK-1 and CD133 appeared at day 20 from cells cultured in dishes without fibronectin coating. In addition, the expression levels of CD133, CD31 and FLK-1/CD133 were not significantly different between cells of different shapes. Our experiments suggest that MNCs from bone marrow can be differentiated into late EP-like cells in EGM-2MV, which have the ability to rapidly proliferate. These MNCs can also be differentiated into early EP-like cells in CM. Additionally, fibronectin may not be necessary for the differentiation of EPCs to mature ECs after three generations. Differentiated MNCs from bone marrow in EGM-2MV have the characteristics of EPCs, although the expression levels of EPC markers were lower than previously reported.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Cell morphology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytology</subject><subject>Endothelial cells</subject><subject>Fibroblast growth factor 2</subject><subject>Fibronectin</subject><subject>Flow cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Laboratory animals</subject><subject>Leukocytes (mononuclear)</subject><subject>Low density lipoprotein</subject><subject>Method in Cell Science</subject><subject>Morphology</subject><subject>Osteoprogenitor cells</subject><subject>Progenitor cells</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular endothelial growth factor receptor 2</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc2KFDEUhYMoTjv6AG4kIOKqND-VSmUjyOAfDLgZ1yGV3HRnqE7aJDXiA_jepuieGRWEQBbnu_eew0HoOSVvKCHybaFkoENHKOkUZ6pjD9CGCsk7IuX4EG2IYk0hgzpDT0q5JoQoSfljdMYo53QQYoN-Xe0A253JxlbIodRgC04eQ3Sp7mAOZsaHnLYQQ00ZW5jngl0jb8Bhn9Me71NMcbEzmFu5jWdT8ZQi4L3JOf3AIWIXvIcMsWK7zHXJ7WyKLtSQYnmKHnkzF3h2-s_Rt48fri4-d5dfP325eH_ZWTGI2vHeCa6kA-UpHaZRgSRisNJIw6l0hoveTK5Bkxu5sO2xkVLPQPhJCCn5OXp33HtYpj0429xkM-tDDs3nT51M0H8rMez0Nt1oTkZK-rEteH1akNP3BUrV-1DW1CZCWooeB963m4w08uU_5HVacmzpNFN0ZENjVooeKZtTKRn8nRdK9NqxPnasW8d67VizNvPizxB3E7elNuDVCTDFmtlnE20o91xP2ajoyrEjV5oUt5DvLf7_-m9mFMID</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Yang, Nana</creator><creator>Li, Dawei</creator><creator>Jiao, Peng</creator><creator>Chen, Bin</creator><creator>Yao, Shutong</creator><creator>Sang, Hui</creator><creator>Yang, Mingfeng</creator><creator>Han, Jiju</creator><creator>Zhang, Ying</creator><creator>Qin, Shucun</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110501</creationdate><title>The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions</title><author>Yang, Nana ; Li, Dawei ; Jiao, Peng ; Chen, Bin ; Yao, Shutong ; Sang, Hui ; Yang, Mingfeng ; Han, Jiju ; Zhang, Ying ; Qin, Shucun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-34d5397de9f116b89e7056c7a7a317da354abd4d5bd835c35c2811f2e5fb55773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Cell morphology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytology</topic><topic>Endothelial cells</topic><topic>Fibroblast growth factor 2</topic><topic>Fibronectin</topic><topic>Flow cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Laboratory animals</topic><topic>Leukocytes (mononuclear)</topic><topic>Low density lipoprotein</topic><topic>Method in Cell Science</topic><topic>Morphology</topic><topic>Osteoprogenitor cells</topic><topic>Progenitor cells</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular endothelial growth factor receptor 2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Nana</creatorcontrib><creatorcontrib>Li, Dawei</creatorcontrib><creatorcontrib>Jiao, Peng</creatorcontrib><creatorcontrib>Chen, Bin</creatorcontrib><creatorcontrib>Yao, Shutong</creatorcontrib><creatorcontrib>Sang, Hui</creatorcontrib><creatorcontrib>Yang, Mingfeng</creatorcontrib><creatorcontrib>Han, Jiju</creatorcontrib><creatorcontrib>Zhang, Ying</creatorcontrib><creatorcontrib>Qin, Shucun</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Nana</au><au>Li, Dawei</au><au>Jiao, Peng</au><au>Chen, Bin</au><au>Yao, Shutong</au><au>Sang, Hui</au><au>Yang, Mingfeng</au><au>Han, Jiju</au><au>Zhang, Ying</au><au>Qin, Shucun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>63</volume><issue>3</issue><spage>217</spage><epage>226</epage><pages>217-226</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Endothelial progenitor cells (EPCs) derived from bone marrow are known to be heterogeneous. In this study, we tried to find favorable conditions that induce the differentiation of mononuclear cells (MNCs) from bone marrow into EPCs. The differentiation capacity of MNCs from rat bone marrow was investigated in different conditions, such as different media, different induction times and different culture surfaces. The cell morphology and endothelial biomarkers associated with differentiated MNCs were studied. Our results indicated that MNCs cultured in EGM-2MV (Endothelial cell basal medium-2, plus SingleQuots of growth supplements) developed a bursiform shape, a late EPC-like morphology, while MNCs cultured in complete medium (CM, M199 with 10% FBS, 20 ng/mL VEGF and 10 ng/mL bFGF) showed a spindle shape, an early EPC-like morphology. Cells of both morphologies were able to incorporate DiI-ac-LDL and bind lectin in vitro. MNCs cultured in EGM-2MV exhibited a higher proliferation rate and higher eNOS expression than MNCs cultured in CM. MNCs cultured in EGM-2MV had the ability to form tubes on Matrigel. Flow cytometry results indicated that CD133 expression was highest at day 12 and that the greatest number of cells positive for both FLK-1 and CD133 appeared at day 20 from cells cultured in dishes without fibronectin coating. In addition, the expression levels of CD133, CD31 and FLK-1/CD133 were not significantly different between cells of different shapes. Our experiments suggest that MNCs from bone marrow can be differentiated into late EP-like cells in EGM-2MV, which have the ability to rapidly proliferate. These MNCs can also be differentiated into early EP-like cells in CM. Additionally, fibronectin may not be necessary for the differentiation of EPCs to mature ECs after three generations. Differentiated MNCs from bone marrow in EGM-2MV have the characteristics of EPCs, although the expression levels of EPC markers were lower than previously reported.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21331655</pmid><doi>10.1007/s10616-010-9329-2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-9069 |
| ispartof | Cytotechnology (Dordrecht), 2011-05, Vol.63 (3), p.217-226 |
| issn | 0920-9069 1573-0778 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3081048 |
| source | SpringerNature Journals; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Antibodies Antigens Biochemistry Biological and medical sciences Biomarkers Biomedicine Biotechnology Bone marrow Cell culture Cell cycle Cell differentiation Cell morphology Chemistry Chemistry and Materials Science Cytology Endothelial cells Fibroblast growth factor 2 Fibronectin Flow cytometry Fundamental and applied biological sciences. Psychology Laboratory animals Leukocytes (mononuclear) Low density lipoprotein Method in Cell Science Morphology Osteoprogenitor cells Progenitor cells Vascular endothelial growth factor Vascular endothelial growth factor receptor 2 |
| title | The characteristics of endothelial progenitor cells derived from mononuclear cells of rat bone marrow in different culture conditions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T10%3A58%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20characteristics%20of%20endothelial%20progenitor%20cells%20derived%20from%20mononuclear%20cells%20of%20rat%20bone%20marrow%20in%20different%20culture%20conditions&rft.jtitle=Cytotechnology%20(Dordrecht)&rft.au=Yang,%20Nana&rft.date=2011-05-01&rft.volume=63&rft.issue=3&rft.spage=217&rft.epage=226&rft.pages=217-226&rft.issn=0920-9069&rft.eissn=1573-0778&rft_id=info:doi/10.1007/s10616-010-9329-2&rft_dat=%3Cproquest_pubme%3E863428120%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2918262030&rft_id=info:pmid/21331655&rfr_iscdi=true |