Expression of blood group genes by mesenchymal stem cells
Summary Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ and protein‐based membrane structures, defined by blood group antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in human serum....
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| Veröffentlicht in: | British journal of haematology 2011-05, Vol.153 (4), p.520-528 |
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| creator | Schäfer, Richard Schnaidt, Martina Klaffschenkel, Roland A. Siegel, Georg Schüle, Michael Rädlein, Maria Anna Hermanutz‐Klein, Ursula Ayturan, Miriam Buadze, Marine Gassner, Christoph Danielyan, Lusine Kluba, Torsten Northoff, Hinnak Flegel, Willy A. |
| description | Summary
Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ and protein‐based membrane structures, defined by blood group antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in human serum. The presence of H (CD173), ABO, RhD, RhCE, RhAG, Kell, urea transporter type B (SLC14A1, previously known as JK), and Duffy antigen receptor of chemokines (DARC) was evaluated at the levels of genome, transcriptome and antigen. Fucosyltransferase‐1 (FUT1), RHCE, KEL, SLC14A1 (JK) and DARC mRNA were transcribed in MSCs. FUT1 mRNA transcription was lost during differentiation. The mRNA transcription of SLC14A1 (JK) decreased during chondrogenic differentiation, while that of DARC increased during adipogenic differentiation. All MSCs synthesized SLC14A1 (JK) but no DARC protein. However, none of the protein antigens tested occurred on the surface, indicating a lack of associated protein function in the membrane. As A and B antigens are neither expressed nor adsorbed, concerns of ABO compatibility with human serum supplements during culture are alleviated. The H antigen expression by GD2dim+ MSCs identified two distinct MSC subpopulations and enabled their isolation. We hypothesize that GD2dim+ H+ MSCs retain a better ‘stemness’. Because immunogenic blood group antigens are lacking, they cannot affect MSC engraftment in vivo, which is promising for clinical applications. |
| doi_str_mv | 10.1111/j.1365-2141.2011.08652.x |
| format | Article |
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Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ and protein‐based membrane structures, defined by blood group antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in human serum. The presence of H (CD173), ABO, RhD, RhCE, RhAG, Kell, urea transporter type B (SLC14A1, previously known as JK), and Duffy antigen receptor of chemokines (DARC) was evaluated at the levels of genome, transcriptome and antigen. Fucosyltransferase‐1 (FUT1), RHCE, KEL, SLC14A1 (JK) and DARC mRNA were transcribed in MSCs. FUT1 mRNA transcription was lost during differentiation. The mRNA transcription of SLC14A1 (JK) decreased during chondrogenic differentiation, while that of DARC increased during adipogenic differentiation. All MSCs synthesized SLC14A1 (JK) but no DARC protein. However, none of the protein antigens tested occurred on the surface, indicating a lack of associated protein function in the membrane. As A and B antigens are neither expressed nor adsorbed, concerns of ABO compatibility with human serum supplements during culture are alleviated. The H antigen expression by GD2dim+ MSCs identified two distinct MSC subpopulations and enabled their isolation. We hypothesize that GD2dim+ H+ MSCs retain a better ‘stemness’. Because immunogenic blood group antigens are lacking, they cannot affect MSC engraftment in vivo, which is promising for clinical applications.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1111/j.1365-2141.2011.08652.x</identifier><identifier>PMID: 21418181</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>ABO Blood-Group System - metabolism ; Biological and medical sciences ; Blood Group Antigens - genetics ; Blood Group Antigens - metabolism ; blood groups ; CD173 ; Cell Differentiation - genetics ; Cells, Cultured ; Duffy Blood-Group System - biosynthesis ; Duffy Blood-Group System - genetics ; Erythrocytes - metabolism ; Gangliosides - metabolism ; H antigen ; Hematologic and hematopoietic diseases ; Humans ; Immunophenotyping ; Medical sciences ; Membrane Transport Proteins - biosynthesis ; Membrane Transport Proteins - genetics ; mesenchymal cells ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - metabolism ; Receptors, Cell Surface - biosynthesis ; Receptors, Cell Surface - genetics ; RNA, Messenger - genetics ; stem cell transplantation ; Transcription, Genetic ; Urea Transporters</subject><ispartof>British journal of haematology, 2011-05, Vol.153 (4), p.520-528</ispartof><rights>Published 2011. This article is a US Government work and is in the public domain in the USA</rights><rights>2015 INIST-CNRS</rights><rights>Published 2011. This article is a US Government work and is in the public domain in the USA.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6022-55c6ba8a762d30d22469a8fa2d54d4c78230992e9f0eb57507fd8bd9b93a9d623</citedby><cites>FETCH-LOGICAL-c6022-55c6ba8a762d30d22469a8fa2d54d4c78230992e9f0eb57507fd8bd9b93a9d623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2141.2011.08652.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2141.2011.08652.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24133010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21418181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schäfer, Richard</creatorcontrib><creatorcontrib>Schnaidt, Martina</creatorcontrib><creatorcontrib>Klaffschenkel, Roland A.</creatorcontrib><creatorcontrib>Siegel, Georg</creatorcontrib><creatorcontrib>Schüle, Michael</creatorcontrib><creatorcontrib>Rädlein, Maria Anna</creatorcontrib><creatorcontrib>Hermanutz‐Klein, Ursula</creatorcontrib><creatorcontrib>Ayturan, Miriam</creatorcontrib><creatorcontrib>Buadze, Marine</creatorcontrib><creatorcontrib>Gassner, Christoph</creatorcontrib><creatorcontrib>Danielyan, Lusine</creatorcontrib><creatorcontrib>Kluba, Torsten</creatorcontrib><creatorcontrib>Northoff, Hinnak</creatorcontrib><creatorcontrib>Flegel, Willy A.</creatorcontrib><title>Expression of blood group genes by mesenchymal stem cells</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>Summary
Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ and protein‐based membrane structures, defined by blood group antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in human serum. The presence of H (CD173), ABO, RhD, RhCE, RhAG, Kell, urea transporter type B (SLC14A1, previously known as JK), and Duffy antigen receptor of chemokines (DARC) was evaluated at the levels of genome, transcriptome and antigen. Fucosyltransferase‐1 (FUT1), RHCE, KEL, SLC14A1 (JK) and DARC mRNA were transcribed in MSCs. FUT1 mRNA transcription was lost during differentiation. The mRNA transcription of SLC14A1 (JK) decreased during chondrogenic differentiation, while that of DARC increased during adipogenic differentiation. All MSCs synthesized SLC14A1 (JK) but no DARC protein. However, none of the protein antigens tested occurred on the surface, indicating a lack of associated protein function in the membrane. As A and B antigens are neither expressed nor adsorbed, concerns of ABO compatibility with human serum supplements during culture are alleviated. The H antigen expression by GD2dim+ MSCs identified two distinct MSC subpopulations and enabled their isolation. We hypothesize that GD2dim+ H+ MSCs retain a better ‘stemness’. Because immunogenic blood group antigens are lacking, they cannot affect MSC engraftment in vivo, which is promising for clinical applications.</description><subject>ABO Blood-Group System - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood Group Antigens - genetics</subject><subject>Blood Group Antigens - metabolism</subject><subject>blood groups</subject><subject>CD173</subject><subject>Cell Differentiation - genetics</subject><subject>Cells, Cultured</subject><subject>Duffy Blood-Group System - biosynthesis</subject><subject>Duffy Blood-Group System - genetics</subject><subject>Erythrocytes - metabolism</subject><subject>Gangliosides - metabolism</subject><subject>H antigen</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Medical sciences</subject><subject>Membrane Transport Proteins - biosynthesis</subject><subject>Membrane Transport Proteins - genetics</subject><subject>mesenchymal cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>Receptors, Cell Surface - biosynthesis</subject><subject>Receptors, Cell Surface - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>stem cell transplantation</subject><subject>Transcription, Genetic</subject><subject>Urea Transporters</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS1ERZfCV0C-ILgknfGfxDmABFWhVJW4wNlybGebVRIv9i7sfnuc7rLABWEfPNL7zdOMHyEUocR8Llcl8koWDAWWDBBLUJVk5e4RWZyEx2QBAHWBINQ5eZrSCgA5SHxCzmdd5bsgzfVuHX1KfZho6Gg7hODoMobtmi795BNt93T0yU_2fj-agaaNH6n1w5CekbPODMk_P74X5OuH6y9XN8Xd54-frt7dFbYCxgopbdUaZeqKOQ6OMVE1RnWGOSmcsLViHJqG-aYD38paQt051bqmbbhpXMX4BXl78F1v29E766dNNINex340ca-D6fXfytTf62X4rjkoEKLOBq-OBjF82_q00WOf5hXM5MM2aVWDlCKjmXz9TxKRSSaVQMyoOqA2hpSi704DIeg5I73ScxR6_mo9Z6QfMtK73Priz4VOjb9CycDLI2CSNUMXzWT79JsTyDkgZO7NgfvRD37_3wPo97c3c8V_Aq2lrBs</recordid><startdate>201105</startdate><enddate>201105</enddate><creator>Schäfer, Richard</creator><creator>Schnaidt, Martina</creator><creator>Klaffschenkel, Roland A.</creator><creator>Siegel, Georg</creator><creator>Schüle, Michael</creator><creator>Rädlein, Maria Anna</creator><creator>Hermanutz‐Klein, Ursula</creator><creator>Ayturan, Miriam</creator><creator>Buadze, Marine</creator><creator>Gassner, Christoph</creator><creator>Danielyan, Lusine</creator><creator>Kluba, Torsten</creator><creator>Northoff, Hinnak</creator><creator>Flegel, Willy A.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201105</creationdate><title>Expression of blood group genes by mesenchymal stem cells</title><author>Schäfer, Richard ; Schnaidt, Martina ; Klaffschenkel, Roland A. ; Siegel, Georg ; Schüle, Michael ; Rädlein, Maria Anna ; Hermanutz‐Klein, Ursula ; Ayturan, Miriam ; Buadze, Marine ; Gassner, Christoph ; Danielyan, Lusine ; Kluba, Torsten ; Northoff, Hinnak ; Flegel, Willy A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6022-55c6ba8a762d30d22469a8fa2d54d4c78230992e9f0eb57507fd8bd9b93a9d623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>ABO Blood-Group System - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blood Group Antigens - genetics</topic><topic>Blood Group Antigens - metabolism</topic><topic>blood groups</topic><topic>CD173</topic><topic>Cell Differentiation - genetics</topic><topic>Cells, Cultured</topic><topic>Duffy Blood-Group System - biosynthesis</topic><topic>Duffy Blood-Group System - genetics</topic><topic>Erythrocytes - metabolism</topic><topic>Gangliosides - metabolism</topic><topic>H antigen</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Medical sciences</topic><topic>Membrane Transport Proteins - biosynthesis</topic><topic>Membrane Transport Proteins - genetics</topic><topic>mesenchymal cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - metabolism</topic><topic>Receptors, Cell Surface - biosynthesis</topic><topic>Receptors, Cell Surface - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>stem cell transplantation</topic><topic>Transcription, Genetic</topic><topic>Urea Transporters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schäfer, Richard</creatorcontrib><creatorcontrib>Schnaidt, Martina</creatorcontrib><creatorcontrib>Klaffschenkel, Roland A.</creatorcontrib><creatorcontrib>Siegel, Georg</creatorcontrib><creatorcontrib>Schüle, Michael</creatorcontrib><creatorcontrib>Rädlein, Maria Anna</creatorcontrib><creatorcontrib>Hermanutz‐Klein, Ursula</creatorcontrib><creatorcontrib>Ayturan, Miriam</creatorcontrib><creatorcontrib>Buadze, Marine</creatorcontrib><creatorcontrib>Gassner, Christoph</creatorcontrib><creatorcontrib>Danielyan, Lusine</creatorcontrib><creatorcontrib>Kluba, Torsten</creatorcontrib><creatorcontrib>Northoff, Hinnak</creatorcontrib><creatorcontrib>Flegel, Willy A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schäfer, Richard</au><au>Schnaidt, Martina</au><au>Klaffschenkel, Roland A.</au><au>Siegel, Georg</au><au>Schüle, Michael</au><au>Rädlein, Maria Anna</au><au>Hermanutz‐Klein, Ursula</au><au>Ayturan, Miriam</au><au>Buadze, Marine</au><au>Gassner, Christoph</au><au>Danielyan, Lusine</au><au>Kluba, Torsten</au><au>Northoff, Hinnak</au><au>Flegel, Willy A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of blood group genes by mesenchymal stem cells</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>2011-05</date><risdate>2011</risdate><volume>153</volume><issue>4</issue><spage>520</spage><epage>528</epage><pages>520-528</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>Summary
Incompatible blood group antigens are highly immunogenic and can cause graft rejections. Focusing on distinct carbohydrate‐ and protein‐based membrane structures, defined by blood group antigens, we investigated human bone marrow‐derived mesenchymal stem cells (MSCs) cultured in human serum. The presence of H (CD173), ABO, RhD, RhCE, RhAG, Kell, urea transporter type B (SLC14A1, previously known as JK), and Duffy antigen receptor of chemokines (DARC) was evaluated at the levels of genome, transcriptome and antigen. Fucosyltransferase‐1 (FUT1), RHCE, KEL, SLC14A1 (JK) and DARC mRNA were transcribed in MSCs. FUT1 mRNA transcription was lost during differentiation. The mRNA transcription of SLC14A1 (JK) decreased during chondrogenic differentiation, while that of DARC increased during adipogenic differentiation. All MSCs synthesized SLC14A1 (JK) but no DARC protein. However, none of the protein antigens tested occurred on the surface, indicating a lack of associated protein function in the membrane. As A and B antigens are neither expressed nor adsorbed, concerns of ABO compatibility with human serum supplements during culture are alleviated. The H antigen expression by GD2dim+ MSCs identified two distinct MSC subpopulations and enabled their isolation. We hypothesize that GD2dim+ H+ MSCs retain a better ‘stemness’. Because immunogenic blood group antigens are lacking, they cannot affect MSC engraftment in vivo, which is promising for clinical applications.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21418181</pmid><doi>10.1111/j.1365-2141.2011.08652.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ABO Blood-Group System - metabolism Biological and medical sciences Blood Group Antigens - genetics Blood Group Antigens - metabolism blood groups CD173 Cell Differentiation - genetics Cells, Cultured Duffy Blood-Group System - biosynthesis Duffy Blood-Group System - genetics Erythrocytes - metabolism Gangliosides - metabolism H antigen Hematologic and hematopoietic diseases Humans Immunophenotyping Medical sciences Membrane Transport Proteins - biosynthesis Membrane Transport Proteins - genetics mesenchymal cells Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Receptors, Cell Surface - biosynthesis Receptors, Cell Surface - genetics RNA, Messenger - genetics stem cell transplantation Transcription, Genetic Urea Transporters |
| title | Expression of blood group genes by mesenchymal stem cells |
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