Expression, purification and crystallization of the Cmi immunity protein from Escherichia coli

Many bacteria kill related bacteria by secretion of bacteriocins. In Escherichia coli, the colicin M protein kills E. coli after uptake into the periplasm. Self‐protection from destruction is provided by the co‐expressed immunity protein. The colicin M immunity protein (Cmi) was cloned, overexpresse...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2011-04, Vol.67 (4), p.517-520
Hauptverfasser:	Römer, Christin, Patzer, Silke I., Albrecht, Reinhard, Zeth, Kornelius, Braun, Volkmar
Format:	Artikel
Sprache:	eng
Schlagworte:	Cmi colicin M Colicins - chemistry Colicins - genetics Colicins - isolation & purification Crystallization Crystallization Communications Crystallography Crystallography, X-Ray Crystals E coli Escherichia coli Escherichia coli - chemistry Gene Expression Immunity immunity proteins Proteins Purification
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