Prevalence of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods
Listeria monocytogenes causes listeriosis characterized by septicaemia, encephalitis, and abortion or stillbirth. Regular monitoring of its prevalence in food and characterization of its phenotypes and genotypes are necessary for disease surveillance and tracing the epidemic outbreaks. In this study...
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| Veröffentlicht in: | Journal of health, population and nutrition population and nutrition, 2011-02, Vol.29 (1), p.26-38 |
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| creator | Indrawattana, Nitaya Nibaddhasobon, Tanaporn Sookrung, Nitat Chongsa-nguan, Manas Tungtrongchitr, Anchalee Makino, Sou-ichi Tungyong, Witawat Chaicumpa, Wanpen |
| description | Listeria monocytogenes causes listeriosis characterized by septicaemia,
encephalitis, and abortion or stillbirth. Regular monitoring of its
prevalence in food and characterization of its phenotypes and genotypes
are necessary for disease surveillance and tracing the epidemic
outbreaks. In this study, the prevalence of L. monocytogenes in raw
meats marketed in Bangkok was 15.4%. The bacteria isolated from meat
were serotyped and genotyped using enterobacterial repetitive
intergenic consensus-polymerase chain reaction (ERIC-PCR). Their
virulence-associated genes, antimicrobial susceptibility, and ability
to invade intestinal epithelial cells were studied. All 22 L.
monocytogenes strains isolated from 104 raw meat samples carried
virulence-associated genes, such as actA, flaA, hlyA, iap, inlA, inlB,
and prfA. These were serotype 4b, suggesting their pathogenic and
epidemic potential. These isolates could be classified into six
ERIC-PCR groups: A-F. The majority (59.1%) of the isolates belonged to
Group A, and three isolates were Group D which was closely related to
the Group A. Two isolates each were Group C and E, and one isolate each
was group B and F. Although the isolates belonged to the same serotype
and genotype and were all equipped with the virulence-associated genes,
they showed a different cell invasion capability and antibiotic
susceptibility. All the isolates were susceptible to ampicillin,
amikacin, chloramphenicol, gentamicin, imipenem, penicillin G,
sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate
showed only intermediate susceptibility to tetracycline. The data
provide the first molecular insight into the L. monocytogenes isolates
in Thailand and elucidate a potential risk of people contracting
listeriosis. |
| doi_str_mv | 10.3329/jhpn.v29i1.7565 |
| format | Article |
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encephalitis, and abortion or stillbirth. Regular monitoring of its
prevalence in food and characterization of its phenotypes and genotypes
are necessary for disease surveillance and tracing the epidemic
outbreaks. In this study, the prevalence of L. monocytogenes in raw
meats marketed in Bangkok was 15.4%. The bacteria isolated from meat
were serotyped and genotyped using enterobacterial repetitive
intergenic consensus-polymerase chain reaction (ERIC-PCR). Their
virulence-associated genes, antimicrobial susceptibility, and ability
to invade intestinal epithelial cells were studied. All 22 L.
monocytogenes strains isolated from 104 raw meat samples carried
virulence-associated genes, such as actA, flaA, hlyA, iap, inlA, inlB,
and prfA. These were serotype 4b, suggesting their pathogenic and
epidemic potential. These isolates could be classified into six
ERIC-PCR groups: A-F. The majority (59.1%) of the isolates belonged to
Group A, and three isolates were Group D which was closely related to
the Group A. Two isolates each were Group C and E, and one isolate each
was group B and F. Although the isolates belonged to the same serotype
and genotype and were all equipped with the virulence-associated genes,
they showed a different cell invasion capability and antibiotic
susceptibility. All the isolates were susceptible to ampicillin,
amikacin, chloramphenicol, gentamicin, imipenem, penicillin G,
sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate
showed only intermediate susceptibility to tetracycline. The data
provide the first molecular insight into the L. monocytogenes isolates
in Thailand and elucidate a potential risk of people contracting
listeriosis.</description><identifier>ISSN: 1606-0997</identifier><identifier>EISSN: 2072-1315</identifier><identifier>DOI: 10.3329/jhpn.v29i1.7565</identifier><identifier>PMID: 21528788</identifier><language>eng</language><publisher>Bangladesh: icddr,b</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Beef ; Characterization ; Disease ; Distribution ; Epidemics ; Flagellin ; Food Microbiology - methods ; Food Microbiology - statistics & numerical data ; Foodborne diseases ; Genes ; Genes typing ; Genotype ; Genotype & phenotype ; Gram-positive bacteria ; Haemolysin ; Health aspects ; Internalins ; Invasins ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - classification ; Listeria monocytogenes - drug effects ; Listeria monocytogenes - pathogenicity ; Listeriolysin ; Listeriosis ; Meat ; Meat - microbiology ; Meats ; Microbial sensitivity tests ; Microbial Sensitivity Tests - methods ; Molecular Sequence Data ; Oligonucleotides ; Open markets ; Original Papers ; Phenotype ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Pork ; Prevalence ; Raw foods ; Risk factors ; Serotyping - methods ; Susceptibility ; Tetracycline ; Thailand ; Virulence ; Virulence genes</subject><ispartof>Journal of health, population and nutrition, 2011-02, Vol.29 (1), p.26-38</ispartof><rights>Copyright 2011 Journal of Health Population and Nutrition.</rights><rights>Copyright © 2011 International Centre for Diarrhoeal Disease Research</rights><rights>COPYRIGHT 2011 BioMed Central Ltd.</rights><rights>Copyright Intenational Centre for Diarrhoeal Disease Research, Bangladesh Feb 2011</rights><rights>INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH, BANGLADESH 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b710t-a9bc89dcccd0244833e4facf635bd1d82069ac1184b2a4d29a638dc1903cbddd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23500073$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23500073$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,12845,27923,27924,30998,30999,53790,53792,58016,58249,79197</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21528788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Indrawattana, Nitaya</creatorcontrib><creatorcontrib>Nibaddhasobon, Tanaporn</creatorcontrib><creatorcontrib>Sookrung, Nitat</creatorcontrib><creatorcontrib>Chongsa-nguan, Manas</creatorcontrib><creatorcontrib>Tungtrongchitr, Anchalee</creatorcontrib><creatorcontrib>Makino, Sou-ichi</creatorcontrib><creatorcontrib>Tungyong, Witawat</creatorcontrib><creatorcontrib>Chaicumpa, Wanpen</creatorcontrib><title>Prevalence of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods</title><title>Journal of health, population and nutrition</title><addtitle>J Health Popul Nutr</addtitle><description>Listeria monocytogenes causes listeriosis characterized by septicaemia,
encephalitis, and abortion or stillbirth. Regular monitoring of its
prevalence in food and characterization of its phenotypes and genotypes
are necessary for disease surveillance and tracing the epidemic
outbreaks. In this study, the prevalence of L. monocytogenes in raw
meats marketed in Bangkok was 15.4%. The bacteria isolated from meat
were serotyped and genotyped using enterobacterial repetitive
intergenic consensus-polymerase chain reaction (ERIC-PCR). Their
virulence-associated genes, antimicrobial susceptibility, and ability
to invade intestinal epithelial cells were studied. All 22 L.
monocytogenes strains isolated from 104 raw meat samples carried
virulence-associated genes, such as actA, flaA, hlyA, iap, inlA, inlB,
and prfA. These were serotype 4b, suggesting their pathogenic and
epidemic potential. These isolates could be classified into six
ERIC-PCR groups: A-F. The majority (59.1%) of the isolates belonged to
Group A, and three isolates were Group D which was closely related to
the Group A. Two isolates each were Group C and E, and one isolate each
was group B and F. Although the isolates belonged to the same serotype
and genotype and were all equipped with the virulence-associated genes,
they showed a different cell invasion capability and antibiotic
susceptibility. All the isolates were susceptible to ampicillin,
amikacin, chloramphenicol, gentamicin, imipenem, penicillin G,
sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate
showed only intermediate susceptibility to tetracycline. The data
provide the first molecular insight into the L. monocytogenes isolates
in Thailand and elucidate a potential risk of people contracting
listeriosis.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Beef</subject><subject>Characterization</subject><subject>Disease</subject><subject>Distribution</subject><subject>Epidemics</subject><subject>Flagellin</subject><subject>Food Microbiology - methods</subject><subject>Food Microbiology - statistics & numerical data</subject><subject>Foodborne diseases</subject><subject>Genes</subject><subject>Genes typing</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Gram-positive bacteria</subject><subject>Haemolysin</subject><subject>Health aspects</subject><subject>Internalins</subject><subject>Invasins</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeria monocytogenes - drug effects</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Listeriolysin</subject><subject>Listeriosis</subject><subject>Meat</subject><subject>Meat - microbiology</subject><subject>Meats</subject><subject>Microbial sensitivity tests</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides</subject><subject>Open markets</subject><subject>Original Papers</subject><subject>Phenotype</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pork</subject><subject>Prevalence</subject><subject>Raw foods</subject><subject>Risk factors</subject><subject>Serotyping - methods</subject><subject>Susceptibility</subject><subject>Tetracycline</subject><subject>Thailand</subject><subject>Virulence</subject><subject>Virulence 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of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods</title><author>Indrawattana, Nitaya ; Nibaddhasobon, Tanaporn ; Sookrung, Nitat ; Chongsa-nguan, Manas ; Tungtrongchitr, Anchalee ; Makino, Sou-ichi ; Tungyong, Witawat ; Chaicumpa, Wanpen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b710t-a9bc89dcccd0244833e4facf635bd1d82069ac1184b2a4d29a638dc1903cbddd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Beef</topic><topic>Characterization</topic><topic>Disease</topic><topic>Distribution</topic><topic>Epidemics</topic><topic>Flagellin</topic><topic>Food Microbiology - methods</topic><topic>Food Microbiology - statistics & numerical data</topic><topic>Foodborne diseases</topic><topic>Genes</topic><topic>Genes typing</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Gram-positive bacteria</topic><topic>Haemolysin</topic><topic>Health aspects</topic><topic>Internalins</topic><topic>Invasins</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeria monocytogenes - drug effects</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Listeriolysin</topic><topic>Listeriosis</topic><topic>Meat</topic><topic>Meat - microbiology</topic><topic>Meats</topic><topic>Microbial sensitivity tests</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotides</topic><topic>Open markets</topic><topic>Original Papers</topic><topic>Phenotype</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pork</topic><topic>Prevalence</topic><topic>Raw 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Indrawattana, Nitaya</au><au>Nibaddhasobon, Tanaporn</au><au>Sookrung, Nitat</au><au>Chongsa-nguan, Manas</au><au>Tungtrongchitr, Anchalee</au><au>Makino, Sou-ichi</au><au>Tungyong, Witawat</au><au>Chaicumpa, Wanpen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods</atitle><jtitle>Journal of health, population and nutrition</jtitle><addtitle>J Health Popul Nutr</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>29</volume><issue>1</issue><spage>26</spage><epage>38</epage><pages>26-38</pages><issn>1606-0997</issn><eissn>2072-1315</eissn><abstract>Listeria monocytogenes causes listeriosis characterized by septicaemia,
encephalitis, and abortion or stillbirth. Regular monitoring of its
prevalence in food and characterization of its phenotypes and genotypes
are necessary for disease surveillance and tracing the epidemic
outbreaks. In this study, the prevalence of L. monocytogenes in raw
meats marketed in Bangkok was 15.4%. The bacteria isolated from meat
were serotyped and genotyped using enterobacterial repetitive
intergenic consensus-polymerase chain reaction (ERIC-PCR). Their
virulence-associated genes, antimicrobial susceptibility, and ability
to invade intestinal epithelial cells were studied. All 22 L.
monocytogenes strains isolated from 104 raw meat samples carried
virulence-associated genes, such as actA, flaA, hlyA, iap, inlA, inlB,
and prfA. These were serotype 4b, suggesting their pathogenic and
epidemic potential. These isolates could be classified into six
ERIC-PCR groups: A-F. The majority (59.1%) of the isolates belonged to
Group A, and three isolates were Group D which was closely related to
the Group A. Two isolates each were Group C and E, and one isolate each
was group B and F. Although the isolates belonged to the same serotype
and genotype and were all equipped with the virulence-associated genes,
they showed a different cell invasion capability and antibiotic
susceptibility. All the isolates were susceptible to ampicillin,
amikacin, chloramphenicol, gentamicin, imipenem, penicillin G,
sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate
showed only intermediate susceptibility to tetracycline. The data
provide the first molecular insight into the L. monocytogenes isolates
in Thailand and elucidate a potential risk of people contracting
listeriosis.</abstract><cop>Bangladesh</cop><pub>icddr,b</pub><pmid>21528788</pmid><doi>10.3329/jhpn.v29i1.7565</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1606-0997 |
| ispartof | Journal of health, population and nutrition, 2011-02, Vol.29 (1), p.26-38 |
| issn | 1606-0997 2072-1315 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3075055 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Applied Social Sciences Index & Abstracts (ASSIA); JSTOR Archive Collection A-Z Listing; Bioline International; PubMed Central |
| subjects | Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Beef Characterization Disease Distribution Epidemics Flagellin Food Microbiology - methods Food Microbiology - statistics & numerical data Foodborne diseases Genes Genes typing Genotype Genotype & phenotype Gram-positive bacteria Haemolysin Health aspects Internalins Invasins Listeria Listeria monocytogenes Listeria monocytogenes - classification Listeria monocytogenes - drug effects Listeria monocytogenes - pathogenicity Listeriolysin Listeriosis Meat Meat - microbiology Meats Microbial sensitivity tests Microbial Sensitivity Tests - methods Molecular Sequence Data Oligonucleotides Open markets Original Papers Phenotype Polymerase chain reaction Polymerase Chain Reaction - methods Pork Prevalence Raw foods Risk factors Serotyping - methods Susceptibility Tetracycline Thailand Virulence Virulence genes |
| title | Prevalence of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T14%3A55%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20of%20Listeria%20monocytogenes%20in%20Raw%20Meats%20Marketed%20in%20Bangkok%20and%20Characterization%20of%20the%20Isolates%20by%20Phenotypic%20and%20Molecular%20Methods&rft.jtitle=Journal%20of%20health,%20population%20and%20nutrition&rft.au=Indrawattana,%20Nitaya&rft.date=2011-02-01&rft.volume=29&rft.issue=1&rft.spage=26&rft.epage=38&rft.pages=26-38&rft.issn=1606-0997&rft.eissn=2072-1315&rft_id=info:doi/10.3329/jhpn.v29i1.7565&rft_dat=%3Cgale_pubme%3EA254014396%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=863812678&rft_id=info:pmid/21528788&rft_galeid=A254014396&rft_jstor_id=23500073&rfr_iscdi=true |