simplified approach for isolating oligo(dT) primed cDNA clones with probes generated by cDNA selection

The cDNA selection method has proven to be an effective approach to identify genes encoded in YACs and has been used successfully in a number of positional cloning efforts. One problem with cDNA selection is that it produces short-fragment cDNA clones. Isolating individual short fragments, determini...

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Veröffentlicht in:	Nucleic acids research 1995-11, Vol.23 (21), p.4528-4529
Hauptverfasser:	Xu, H, Wei, H, Kolluri, R, Wiessman, S.M
Format:	Artikel
Sprache:	eng
Schlagworte:	biochemical techniques Blotting, Southern Chromosomes, Artificial, Yeast - genetics Cloning, Molecular - methods complementary DNA DNA Primers DNA Probes DNA, Complementary - genetics Gene Library Humans isolation Nucleic Acid Hybridization Oligodeoxyribonucleotides T-Lymphocytes
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