Reconstitution of human RNA interference in budding yeast
Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether th...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 2011-04, Vol.39 (7), p.e43-e43 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e43 |
|---|---|
| container_issue | 7 |
| container_start_page | e43 |
| container_title | Nucleic acids research |
| container_volume | 39 |
| creator | Suk, Kyoungho Choi, Jihye Suzuki, Yo Ozturk, Sedide B Mellor, Joseph C Wong, Koon Ho MacKay, Joanna L Gregory, Richard I Roth, Frederick P |
| description | Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether the introduction of human RNAi genes can reconstitute RNAi in S. cerevisiae, genes encoding these three human proteins were introduced into S. cerevisiae. We observed both siRNA and siRNA- and RISC-dependent silencing of the target gene GFP. Thus, human Ago2, Dicer and TRBP can functionally reconstitute human RNAi in S. cerevisiae, in vivo, enabling the study and use of the human RNAi pathway in a facile genetic model organism. |
| doi_str_mv | 10.1093/nar/gkq1321 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3074155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>869794240</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-411638edaa053087abe695289c4697c23550e7f8a56df81d72c1b24bc6671d433</originalsourceid><addsrcrecordid>eNqNkU1LAzEQhoMotlZP3mVvHmRtJh-7yUUoxS8oCkXPIZvNtqvbbJtkhf57V1pFb56GYR5e3uFB6BzwNWBJx0778eJ9A5TAARoCzUjKZEYO0RBTzFPATAzQSQhvGAMDzo7RgADhhEg6RHJuTetCrGMX69YlbZUsu5V2yfxpktQuWl9Zb52x_ZIUXVnWbpFsrQ7xFB1Vugn2bD9H6PXu9mX6kM6e7x-nk1lqWC5iygAyKmypNeYUi1wXNpOcCGlYJnNDKOfY5pXQPCsrAWVODBSEFSbLcigZpSN0s8tdd8XKlsa66HWj1r5eab9Vra7V34url2rRfiiK8_5d3gdc7gN8u-lsiGpVB2ObRjvbdkEJKYEyCv8g-8aSEYZ78mpHGt-G4G310wew-rKieitqb6WnL36_8MN-a6CfxIiIzA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>869794240</pqid></control><display><type>article</type><title>Reconstitution of human RNA interference in budding yeast</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Oxford Journals Open Access Collection</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Suk, Kyoungho ; Choi, Jihye ; Suzuki, Yo ; Ozturk, Sedide B ; Mellor, Joseph C ; Wong, Koon Ho ; MacKay, Joanna L ; Gregory, Richard I ; Roth, Frederick P</creator><creatorcontrib>Suk, Kyoungho ; Choi, Jihye ; Suzuki, Yo ; Ozturk, Sedide B ; Mellor, Joseph C ; Wong, Koon Ho ; MacKay, Joanna L ; Gregory, Richard I ; Roth, Frederick P</creatorcontrib><description>Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether the introduction of human RNAi genes can reconstitute RNAi in S. cerevisiae, genes encoding these three human proteins were introduced into S. cerevisiae. We observed both siRNA and siRNA- and RISC-dependent silencing of the target gene GFP. Thus, human Ago2, Dicer and TRBP can functionally reconstitute human RNAi in S. cerevisiae, in vivo, enabling the study and use of the human RNAi pathway in a facile genetic model organism.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gkq1321</identifier><identifier>PMID: 21252293</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Argonaute Proteins ; Eukaryotic Initiation Factor-2 - genetics ; Eukaryotic Initiation Factor-2 - metabolism ; Humans ; Methods Online ; Models, Genetic ; Ribonuclease III - genetics ; Ribonuclease III - metabolism ; RNA Interference ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism</subject><ispartof>Nucleic acids research, 2011-04, Vol.39 (7), p.e43-e43</ispartof><rights>The Author(s) 2011. Published by Oxford University Press. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-411638edaa053087abe695289c4697c23550e7f8a56df81d72c1b24bc6671d433</citedby><cites>FETCH-LOGICAL-c478t-411638edaa053087abe695289c4697c23550e7f8a56df81d72c1b24bc6671d433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074155/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074155/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21252293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suk, Kyoungho</creatorcontrib><creatorcontrib>Choi, Jihye</creatorcontrib><creatorcontrib>Suzuki, Yo</creatorcontrib><creatorcontrib>Ozturk, Sedide B</creatorcontrib><creatorcontrib>Mellor, Joseph C</creatorcontrib><creatorcontrib>Wong, Koon Ho</creatorcontrib><creatorcontrib>MacKay, Joanna L</creatorcontrib><creatorcontrib>Gregory, Richard I</creatorcontrib><creatorcontrib>Roth, Frederick P</creatorcontrib><title>Reconstitution of human RNA interference in budding yeast</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether the introduction of human RNAi genes can reconstitute RNAi in S. cerevisiae, genes encoding these three human proteins were introduced into S. cerevisiae. We observed both siRNA and siRNA- and RISC-dependent silencing of the target gene GFP. Thus, human Ago2, Dicer and TRBP can functionally reconstitute human RNAi in S. cerevisiae, in vivo, enabling the study and use of the human RNAi pathway in a facile genetic model organism.</description><subject>Argonaute Proteins</subject><subject>Eukaryotic Initiation Factor-2 - genetics</subject><subject>Eukaryotic Initiation Factor-2 - metabolism</subject><subject>Humans</subject><subject>Methods Online</subject><subject>Models, Genetic</subject><subject>Ribonuclease III - genetics</subject><subject>Ribonuclease III - metabolism</subject><subject>RNA Interference</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1LAzEQhoMotlZP3mVvHmRtJh-7yUUoxS8oCkXPIZvNtqvbbJtkhf57V1pFb56GYR5e3uFB6BzwNWBJx0778eJ9A5TAARoCzUjKZEYO0RBTzFPATAzQSQhvGAMDzo7RgADhhEg6RHJuTetCrGMX69YlbZUsu5V2yfxpktQuWl9Zb52x_ZIUXVnWbpFsrQ7xFB1Vugn2bD9H6PXu9mX6kM6e7x-nk1lqWC5iygAyKmypNeYUi1wXNpOcCGlYJnNDKOfY5pXQPCsrAWVODBSEFSbLcigZpSN0s8tdd8XKlsa66HWj1r5eab9Vra7V34url2rRfiiK8_5d3gdc7gN8u-lsiGpVB2ObRjvbdkEJKYEyCv8g-8aSEYZ78mpHGt-G4G310wew-rKieitqb6WnL36_8MN-a6CfxIiIzA</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Suk, Kyoungho</creator><creator>Choi, Jihye</creator><creator>Suzuki, Yo</creator><creator>Ozturk, Sedide B</creator><creator>Mellor, Joseph C</creator><creator>Wong, Koon Ho</creator><creator>MacKay, Joanna L</creator><creator>Gregory, Richard I</creator><creator>Roth, Frederick P</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20110401</creationdate><title>Reconstitution of human RNA interference in budding yeast</title><author>Suk, Kyoungho ; Choi, Jihye ; Suzuki, Yo ; Ozturk, Sedide B ; Mellor, Joseph C ; Wong, Koon Ho ; MacKay, Joanna L ; Gregory, Richard I ; Roth, Frederick P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-411638edaa053087abe695289c4697c23550e7f8a56df81d72c1b24bc6671d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Argonaute Proteins</topic><topic>Eukaryotic Initiation Factor-2 - genetics</topic><topic>Eukaryotic Initiation Factor-2 - metabolism</topic><topic>Humans</topic><topic>Methods Online</topic><topic>Models, Genetic</topic><topic>Ribonuclease III - genetics</topic><topic>Ribonuclease III - metabolism</topic><topic>RNA Interference</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suk, Kyoungho</creatorcontrib><creatorcontrib>Choi, Jihye</creatorcontrib><creatorcontrib>Suzuki, Yo</creatorcontrib><creatorcontrib>Ozturk, Sedide B</creatorcontrib><creatorcontrib>Mellor, Joseph C</creatorcontrib><creatorcontrib>Wong, Koon Ho</creatorcontrib><creatorcontrib>MacKay, Joanna L</creatorcontrib><creatorcontrib>Gregory, Richard I</creatorcontrib><creatorcontrib>Roth, Frederick P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suk, Kyoungho</au><au>Choi, Jihye</au><au>Suzuki, Yo</au><au>Ozturk, Sedide B</au><au>Mellor, Joseph C</au><au>Wong, Koon Ho</au><au>MacKay, Joanna L</au><au>Gregory, Richard I</au><au>Roth, Frederick P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reconstitution of human RNA interference in budding yeast</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>39</volume><issue>7</issue><spage>e43</spage><epage>e43</epage><pages>e43-e43</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Although RNA-mediated interference (RNAi) is a widely conserved process among eukaryotes, including many fungi, it is absent from the budding yeast Saccharomyces cerevisiae. Three human proteins, Ago2, Dicer and TRBP, are sufficient for reconstituting the RISC complex in vitro. To examine whether the introduction of human RNAi genes can reconstitute RNAi in S. cerevisiae, genes encoding these three human proteins were introduced into S. cerevisiae. We observed both siRNA and siRNA- and RISC-dependent silencing of the target gene GFP. Thus, human Ago2, Dicer and TRBP can functionally reconstitute human RNAi in S. cerevisiae, in vivo, enabling the study and use of the human RNAi pathway in a facile genetic model organism.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>21252293</pmid><doi>10.1093/nar/gkq1321</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 2011-04, Vol.39 (7), p.e43-e43 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3074155 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Oxford Journals Open Access Collection; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Argonaute Proteins Eukaryotic Initiation Factor-2 - genetics Eukaryotic Initiation Factor-2 - metabolism Humans Methods Online Models, Genetic Ribonuclease III - genetics Ribonuclease III - metabolism RNA Interference RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism |
| title | Reconstitution of human RNA interference in budding yeast |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T17%3A57%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reconstitution%20of%20human%20RNA%20interference%20in%20budding%20yeast&rft.jtitle=Nucleic%20acids%20research&rft.au=Suk,%20Kyoungho&rft.date=2011-04-01&rft.volume=39&rft.issue=7&rft.spage=e43&rft.epage=e43&rft.pages=e43-e43&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/gkq1321&rft_dat=%3Cproquest_pubme%3E869794240%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=869794240&rft_id=info:pmid/21252293&rfr_iscdi=true |