Interactions of calf thymus DNA polymerase α with primer/templates

The interactions of calf thymus DNA polymerase α (pol α) with primer/templates were examined. Simply changing the primer from DNA to RNA had little effect on primer/template binding or dNTP polymerization (Km, Vmax and processivity). Surprisingly, however, adding a 5′-triphosphate to the primer grea...

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Veröffentlicht in:	Nucleic acids research 1995-10, Vol.23 (20), p.4109-4115
Hauptverfasser:	Thompson, Harry C., Sheaff, Robert J., Kuchta, Robert D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Composition Base Sequence Cattle Deoxyribonuclease I DNA Polymerase II - metabolism DNA Primers - chemical synthesis DNA Primers - chemistry DNA Primers - metabolism Kinetics Molecular Sequence Data Polyphosphates RNA - chemical synthesis RNA - chemistry RNA - metabolism Templates, Genetic Thymus Gland - enzymology
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