Next-generation high-density self-assembling functional protein arrays

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high y...

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Veröffentlicht in:Nature methods 2008-06, Vol.5 (6), p.535-538
Hauptverfasser: LaBaer, Joshua, Ramachandran, Niroshan, Raphael, Jacob V, Hainsworth, Eugenie, Demirkan, Gokhan, Fuentes, Manuel G, Rolfs, Andreas, Hu, Yanhui
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container_end_page 538
container_issue 6
container_start_page 535
container_title Nature methods
container_volume 5
creator LaBaer, Joshua
Ramachandran, Niroshan
Raphael, Jacob V
Hainsworth, Eugenie
Demirkan, Gokhan
Fuentes, Manuel G
Rolfs, Andreas
Hu, Yanhui
description We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
doi_str_mv 10.1038/nmeth.1210
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subjects Albumins - chemistry
Animals
Antisense DNA
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
brief-communication
Cattle
Cell-Free System
Cellular proteins
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA, Complementary - metabolism
Experiments
Fluorescent Dyes - pharmacology
Gene Expression Profiling - instrumentation
Gene Expression Profiling - methods
Genetic aspects
Glutathione Transferase - metabolism
Humans
Life Sciences
Miniaturization
Nucleic acids
Oligonucleotide Array Sequence Analysis
Peptide Library
Physiological aspects
Protein Array Analysis - instrumentation
Protein Array Analysis - methods
Protein microarrays
Proteins
Proteomics
Proteomics - methods
Proteomics - trends
Research methodology
title Next-generation high-density self-assembling functional protein arrays
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