Next-generation high-density self-assembling functional protein arrays
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high y...
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Veröffentlicht in: | Nature methods 2008-06, Vol.5 (6), p.535-538 |
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creator | LaBaer, Joshua Ramachandran, Niroshan Raphael, Jacob V Hainsworth, Eugenie Demirkan, Gokhan Fuentes, Manuel G Rolfs, Andreas Hu, Yanhui |
description | We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput. |
doi_str_mv | 10.1038/nmeth.1210 |
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We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth.1210</identifier><identifier>PMID: 18469824</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Albumins - chemistry ; Animals ; Antisense DNA ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; brief-communication ; Cattle ; Cell-Free System ; Cellular proteins ; Cloning, Molecular ; Deoxyribonucleic acid ; DNA ; DNA, Complementary - metabolism ; Experiments ; Fluorescent Dyes - pharmacology ; Gene Expression Profiling - instrumentation ; Gene Expression Profiling - methods ; Genetic aspects ; Glutathione Transferase - metabolism ; Humans ; Life Sciences ; Miniaturization ; Nucleic acids ; Oligonucleotide Array Sequence Analysis ; Peptide Library ; Physiological aspects ; Protein Array Analysis - instrumentation ; Protein Array Analysis - methods ; Protein microarrays ; Proteins ; Proteomics ; Proteomics - methods ; Proteomics - trends ; Research methodology</subject><ispartof>Nature methods, 2008-06, Vol.5 (6), p.535-538</ispartof><rights>Springer Nature America, Inc. 2008</rights><rights>COPYRIGHT 2008 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-aea0fa43810d061cdf4b9b0ee3bd95849c000c52b0dd5a231384a2f4e986a4e03</citedby><cites>FETCH-LOGICAL-c562t-aea0fa43810d061cdf4b9b0ee3bd95849c000c52b0dd5a231384a2f4e986a4e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,2727,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18469824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LaBaer, Joshua</creatorcontrib><creatorcontrib>Ramachandran, Niroshan</creatorcontrib><creatorcontrib>Raphael, Jacob V</creatorcontrib><creatorcontrib>Hainsworth, Eugenie</creatorcontrib><creatorcontrib>Demirkan, Gokhan</creatorcontrib><creatorcontrib>Fuentes, Manuel G</creatorcontrib><creatorcontrib>Rolfs, Andreas</creatorcontrib><creatorcontrib>Hu, Yanhui</creatorcontrib><title>Next-generation high-density self-assembling functional protein arrays</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.</description><subject>Albumins - chemistry</subject><subject>Animals</subject><subject>Antisense DNA</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>brief-communication</subject><subject>Cattle</subject><subject>Cell-Free System</subject><subject>Cellular proteins</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Complementary - metabolism</subject><subject>Experiments</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Gene Expression Profiling - instrumentation</subject><subject>Gene Expression Profiling - methods</subject><subject>Genetic aspects</subject><subject>Glutathione Transferase - metabolism</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Miniaturization</subject><subject>Nucleic acids</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Peptide Library</subject><subject>Physiological aspects</subject><subject>Protein Array Analysis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LaBaer, Joshua</au><au>Ramachandran, Niroshan</au><au>Raphael, Jacob V</au><au>Hainsworth, Eugenie</au><au>Demirkan, Gokhan</au><au>Fuentes, Manuel G</au><au>Rolfs, Andreas</au><au>Hu, Yanhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Next-generation high-density self-assembling functional protein arrays</atitle><jtitle>Nature methods</jtitle><stitle>Nat Methods</stitle><addtitle>Nat Methods</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>5</volume><issue>6</issue><spage>535</spage><epage>538</epage><pages>535-538</pages><issn>1548-7091</issn><eissn>1548-7105</eissn><abstract>We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>18469824</pmid><doi>10.1038/nmeth.1210</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Albumins - chemistry Animals Antisense DNA Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cattle Cell-Free System Cellular proteins Cloning, Molecular Deoxyribonucleic acid DNA DNA, Complementary - metabolism Experiments Fluorescent Dyes - pharmacology Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Genetic aspects Glutathione Transferase - metabolism Humans Life Sciences Miniaturization Nucleic acids Oligonucleotide Array Sequence Analysis Peptide Library Physiological aspects Protein Array Analysis - instrumentation Protein Array Analysis - methods Protein microarrays Proteins Proteomics Proteomics - methods Proteomics - trends Research methodology |
title | Next-generation high-density self-assembling functional protein arrays |
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