Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique
AIM：To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS：Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2011-03, Vol.17 (11), p.1442-1447 |
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| creator | Li, Yan Li, Bo Zhang, Yu Xiang, Chun-Ping Li, Yuan-Yuan Wu, Xiao-Ling |
| description | AIM：To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS：Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor tissue pieces were implanted under the serous coat.The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.RESULTS：Within 2-4 wk,there were no obvious chang-es about the primary tumor in stomach.At the sixth week,the primary tumor began to grow fast,resulting in incrassation of the gastric wall and stenosis of the gastric cavity,and metastases into the liver and lymph nodes were detected.The tumor,which compressed the adjacent organs,gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk.There were massive metastases,and the rate of metastasis was 58%in lymph nodes,78%in liver,39%in kidney,and 81%in peritoneum or septum.CONCLUSION：A gastric cancer model is established,which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment. |
| doi_str_mv | 10.3748/wjg.v17.i11.1442 |
| format | Article |
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All rights reserved. 2011</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-658c81488f635e6b0ecd10e545a461590573269810b080cd9b11dfde24afadfb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070017/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070017/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21472102$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Xiang, Chun-Ping</creatorcontrib><creatorcontrib>Li, Yuan-Yuan</creatorcontrib><creatorcontrib>Wu, Xiao-Ling</creatorcontrib><title>Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM：To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS：Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor tissue pieces were implanted under the serous coat.The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.RESULTS：Within 2-4 wk,there were no obvious chang-es about the primary tumor in stomach.At the sixth week,the primary tumor began to grow fast,resulting in incrassation of the gastric wall and stenosis of the gastric cavity,and metastases into the liver and lymph nodes were detected.The tumor,which compressed the adjacent organs,gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk.There were massive metastases,and the rate of metastasis was 58%in lymph nodes,78%in liver,39%in kidney,and 81%in peritoneum or septum.CONCLUSION：A gastric cancer model is established,which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment.</description><subject>Animals</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Disease Models, Animal</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Keratin-20 - metabolism</subject><subject>Kidney Neoplasms - secondary</subject><subject>Liver Neoplasms - secondary</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Mucin-1 - metabolism</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Transplantation</subject><subject>Original</subject><subject>Peritoneal Neoplasms - secondary</subject><subject>Stomach Neoplasms - blood supply</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Time Factors</subject><subject>Tumor Burden</subject><subject>串行</subject><subject>临床试验</subject><subject>原位</subject><subject>小鼠模型</subject><subject>技术</subject><subject>植入</subject><subject>肿瘤组织</subject><subject>胃癌</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU2PFCEQhonRuOPq3ZPBk6ceq4Bu6IuJ2awfySYe1DOhabqHtQdmgR7jv5d1x4meCsJbDwUPIS8RtlwK9fbn7bw9otx6xC0KwR6RDWPYN0wJeEw2CCCbnjN5QZ7lfAvAOG_ZU3LBUEiGwDbkx1eXvFloHLJLR1N8DJnGQE2gMZVdLPHgLZ1NLqlWa4J1ie7j6BZqa7Sk1RY30jX7MFO_P6R4rNu6WEwof3C0OLsL_m51z8mTySzZvTjVS_L9w_W3q0_NzZePn6_e3zRWKFmarlVWoVBq6njrugGcHRFcK1ojOmx7aCVnXa8QBlBgx35AHKfRMWEmM04DvyTvHriHddi70bpQkln0Ifm9Sb90NF7_fxL8Ts_xqDlIAJQV8OYESLHOnYve-2zdUt_k4pq16pABE6BqEh6SNsWck5vOtyDoe0W6KtJVka6K9L2i2vLq3-nODX-d1MDrE3MXw3xXP_ac4VK1gvXIfwMUnZ0K</recordid><startdate>20110321</startdate><enddate>20110321</enddate><creator>Li, Yan</creator><creator>Li, Bo</creator><creator>Zhang, Yu</creator><creator>Xiang, Chun-Ping</creator><creator>Li, Yuan-Yuan</creator><creator>Wu, Xiao-Ling</creator><general>Baishideng Publishing Group Co., Limited</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110321</creationdate><title>Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique</title><author>Li, Yan ; Li, Bo ; Zhang, Yu ; Xiang, Chun-Ping ; Li, Yuan-Yuan ; Wu, Xiao-Ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-658c81488f635e6b0ecd10e545a461590573269810b080cd9b11dfde24afadfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Disease Models, Animal</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Keratin-20 - metabolism</topic><topic>Kidney Neoplasms - secondary</topic><topic>Liver Neoplasms - secondary</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Mucin-1 - metabolism</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Transplantation</topic><topic>Original</topic><topic>Peritoneal Neoplasms - secondary</topic><topic>Stomach Neoplasms - blood supply</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Time Factors</topic><topic>Tumor Burden</topic><topic>串行</topic><topic>临床试验</topic><topic>原位</topic><topic>小鼠模型</topic><topic>技术</topic><topic>植入</topic><topic>肿瘤组织</topic><topic>胃癌</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Xiang, Chun-Ping</creatorcontrib><creatorcontrib>Li, Yuan-Yuan</creatorcontrib><creatorcontrib>Wu, Xiao-Ling</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yan</au><au>Li, Bo</au><au>Zhang, Yu</au><au>Xiang, Chun-Ping</au><au>Li, Yuan-Yuan</au><au>Wu, Xiao-Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2011-03-21</date><risdate>2011</risdate><volume>17</volume><issue>11</issue><spage>1442</spage><epage>1447</epage><pages>1442-1447</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM：To establish a gastric cancer nude-mouse model with improved orthotopic implantation and investigate its biological characteristics at different time points.METHODS：Human gastric cancer SGC-7901 cell suspensions were injected subcutaneously into a nude mouse to develop solid tumors,and the tumor tissue pieces were implanted under the serous coat.The nude mice were then euthanized in group every two weeks to observe the primary tumor growth and metastases.RESULTS：Within 2-4 wk,there were no obvious chang-es about the primary tumor in stomach.At the sixth week,the primary tumor began to grow fast,resulting in incrassation of the gastric wall and stenosis of the gastric cavity,and metastases into the liver and lymph nodes were detected.The tumor,which compressed the adjacent organs,gradually became bigger and bigger followed by stenosis or vanishment of the gastric cavity from 8 to 12 wk.There were massive metastases,and the rate of metastasis was 58%in lymph nodes,78%in liver,39%in kidney,and 81%in peritoneum or septum.CONCLUSION：A gastric cancer model is established,which can simulate the clinical tumor behavior and provide experimental carrier for clinical trials of gastric cancer treatment.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Co., Limited</pub><pmid>21472102</pmid><doi>10.3748/wjg.v17.i11.1442</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2011-03, Vol.17 (11), p.1442-1447 |
| issn | 1007-9327 2219-2840 |
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| source | MEDLINE; Baishideng "World Journal of" online journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biomarkers, Tumor - metabolism Cell Line, Tumor Disease Models, Animal Humans Immunohistochemistry Keratin-20 - metabolism Kidney Neoplasms - secondary Liver Neoplasms - secondary Lymphatic Metastasis Male Mice Mice, Inbred BALB C Mice, Nude Mucin-1 - metabolism Neoplasm Invasiveness Neoplasm Transplantation Original Peritoneal Neoplasms - secondary Stomach Neoplasms - blood supply Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Time Factors Tumor Burden 串行 临床试验 原位 小鼠模型 技术 植入 肿瘤组织 胃癌 |
| title | Serial observations on an orthotopic gastric cancer model constructed using improved implantation technique |
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