Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells

In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin − CD34 + CD43 + CD45 + multipotent progenitors. The protocol comprises three maj...

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Veröffentlicht in:	Nature protocols 2011-03, Vol.6 (3), p.296-313
Hauptverfasser:	Slukvin, Igor I, Choi, Kyung-Dal, Vodyanik, Maxim
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1407/651 631/250/2504/133/2505 631/532/1360 Analytical Chemistry Biological Techniques Biomedical and Life Sciences Bone marrow Cell Differentiation Coculture Techniques - methods Computational Biology/Bioinformatics Cytokines Dendritic cells Drug dosages Drug testing Granulocyte-macrophage colony stimulating factor Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Granulocytes Hematopoiesis - physiology Hematopoietic stem cells Humans Life Sciences Microarrays Myeloid Progenitor Cells - cytology Myeloid Progenitor Cells - metabolism Neutrophils Organic Chemistry Physiological aspects Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism protocol Stem cells Stromal Cells - cytology Stromal Cells - metabolism Time Factors
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