Genomic characterization of the human DNA excision repair gene ERCC-1
In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Cl...
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| Veröffentlicht in: | Nucleic acids research 1987-11, Vol.15 (22), p.9195-9213 |
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| container_title | Nucleic acids research |
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| creator | VAN DUIN, M KOKEN, M. H. M VAN DEN TOL, J TEN DIJKE, P ODIJK, H WESTERVELD, A BOOTSMA, D HŒIJMAKERS, J. H. J |
| description | In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function. |
| doi_str_mv | 10.1093/nar/15.22.9195 |
| format | Article |
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H. M ; VAN DEN TOL, J ; TEN DIJKE, P ; ODIJK, H ; WESTERVELD, A ; BOOTSMA, D ; HŒIJMAKERS, J. H. J</creator><creatorcontrib>VAN DUIN, M ; KOKEN, M. H. M ; VAN DEN TOL, J ; TEN DIJKE, P ; ODIJK, H ; WESTERVELD, A ; BOOTSMA, D ; HŒIJMAKERS, J. H. J</creatorcontrib><description>In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/15.22.9195</identifier><identifier>PMID: 3684592</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell Line ; DNA - isolation & purification ; DNA Repair ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes. Genome ; HeLa Cells - metabolism ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Plasmids ; Promoter Regions, Genetic ; Transfection ; Ultraviolet Rays</subject><ispartof>Nucleic acids research, 1987-11, Vol.15 (22), p.9195-9213</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-25016f0d70c0ada62f4c95de791b389ecffed358a202a91be66c2e2db56667393</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC306462/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC306462/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7508466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3684592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VAN DUIN, M</creatorcontrib><creatorcontrib>KOKEN, M. H. M</creatorcontrib><creatorcontrib>VAN DEN TOL, J</creatorcontrib><creatorcontrib>TEN DIJKE, P</creatorcontrib><creatorcontrib>ODIJK, H</creatorcontrib><creatorcontrib>WESTERVELD, A</creatorcontrib><creatorcontrib>BOOTSMA, D</creatorcontrib><creatorcontrib>HŒIJMAKERS, J. H. J</creatorcontrib><title>Genomic characterization of the human DNA excision repair gene ERCC-1</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>DNA - isolation & purification</subject><subject>DNA Repair</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Transfection</subject><subject>Ultraviolet Rays</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1r20AQhpfS4jhpr70FdCi5yd5vaQ85GNdNAiaBkJ6X8Wpkb5FWzq5cmv76ytiY5JSeBvZ93mGWh5CvjE4YNWIaIE6ZmnA-McyoD2TMhOa5NJp_JGMqqMoZleUZOU_pF6VMMiVHZCR0KZXhY7K4wdC13mVuAxFcj9H_hd53IevqrN9gttm1ELLv97MM_zif9knELfiYrTFgtnicz3P2mXyqoUn45TgvyM8fi6f5bb58uLmbz5a5k0L1OVeU6ZpWBXUUKtC8ls6oCgvDVqI06OoaK6FK4JTD8IZaO468WimtdSGMuCDXh73b3arFymHoIzR2G30L8cV24O3bJPiNXXe_raBaaj70r4792D3vMPW29clh00DAbpdsUZSyEEy_CzKjBRXmP0BZSiHL_emTA-hil1LE-nQ1o3Zv0g4mLVOWc7s3ORQuX__1hB_VDfm3Yw7JQVNHCIOfE1YoWkqtxT_nxaYf</recordid><startdate>19871125</startdate><enddate>19871125</enddate><creator>VAN DUIN, M</creator><creator>KOKEN, M. H. M</creator><creator>VAN DEN TOL, J</creator><creator>TEN DIJKE, P</creator><creator>ODIJK, H</creator><creator>WESTERVELD, A</creator><creator>BOOTSMA, D</creator><creator>HŒIJMAKERS, J. H. J</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871125</creationdate><title>Genomic characterization of the human DNA excision repair gene ERCC-1</title><author>VAN DUIN, M ; KOKEN, M. H. M ; VAN DEN TOL, J ; TEN DIJKE, P ; ODIJK, H ; WESTERVELD, A ; BOOTSMA, D ; HŒIJMAKERS, J. H. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-25016f0d70c0ada62f4c95de791b389ecffed358a202a91be66c2e2db56667393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>DNA - isolation & purification</topic><topic>DNA Repair</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Transfection</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VAN DUIN, M</creatorcontrib><creatorcontrib>KOKEN, M. H. M</creatorcontrib><creatorcontrib>VAN DEN TOL, J</creatorcontrib><creatorcontrib>TEN DIJKE, P</creatorcontrib><creatorcontrib>ODIJK, H</creatorcontrib><creatorcontrib>WESTERVELD, A</creatorcontrib><creatorcontrib>BOOTSMA, D</creatorcontrib><creatorcontrib>HŒIJMAKERS, J. H. 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J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic characterization of the human DNA excision repair gene ERCC-1</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1987-11-25</date><risdate>1987</risdate><volume>15</volume><issue>22</issue><spage>9195</spage><epage>9213</epage><pages>9195-9213</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3684592</pmid><doi>10.1093/nar/15.22.9195</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Biological and medical sciences Cell Line DNA - isolation & purification DNA Repair Fundamental and applied biological sciences. Psychology Genes Genes. Genome HeLa Cells - metabolism Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Plasmids Promoter Regions, Genetic Transfection Ultraviolet Rays |
| title | Genomic characterization of the human DNA excision repair gene ERCC-1 |
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