5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay
Ribosome biogenesis requires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and eliminate defective products of transcription and processing. We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degrada...
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| Veröffentlicht in: | Nucleic acids research 2011-03, Vol.39 (5), p.1811-1822 |
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| creator | Wang, Minshi Pestov, Dimitri G |
| description | Ribosome biogenesis requires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and eliminate defective products of transcription and processing. We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage events that deviate from the main processing pathway. We propose that probing of pre-rRNA maturation intermediates by exonucleases serves the dual function of generating mature rRNAs and suppressing suboptimal processing paths during ribosome assembly. |
| doi_str_mv | 10.1093/nar/gkq1050 |
| format | Article |
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We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage events that deviate from the main processing pathway. 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We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage events that deviate from the main processing pathway. We propose that probing of pre-rRNA maturation intermediates by exonucleases serves the dual function of generating mature rRNAs and suppressing suboptimal processing paths during ribosome assembly.</description><subject>Animals</subject><subject>Exoribonucleases - antagonists & inhibitors</subject><subject>Exoribonucleases - physiology</subject><subject>Mice</subject><subject>NIH 3T3 Cells</subject><subject>Nuclear Proteins - physiology</subject><subject>RNA</subject><subject>RNA Precursors - metabolism</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA Stability</subject><subject>RNA, Ribosomal - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9L5TAQx4Os6Fv15F1y87B0zY8mTS4LIqu7IAqi4C1M06mva5s-k1Z4_70Rn7KehIGBmQ9f5jtfQg45-8mZlScB4snD4xNnim2RBZdaFKXV4htZMMlUwVlpdsn3lP4xxkuuyh2yKziT2lR8QWp1XGBoaJrjM3Z9D8Ejrdf0PgZBwU-JQi6alhCxoQP6JYQuDbQdIx1gGKDvINBVxCLeXJ3m0TRHmLoxUMiqDXpY75PtFvqEB5u-R-7Of9-e_Skury_-np1eFr6szFQgGNGgsMhl67Wv67JSgBoZIC-bWinr0UJTWaGNgOzDlFWbXXhkUlmh5B759aa7musBG49hitC7VewGiGs3Quc-b0K3dA_js5NMc6ZZFjjeCMTxacY0uaFLHl-fguOcnLGWa861-ppURpj83yqTP95IH8eUIrYf93DmXuNzOT63iS_TR_9b-GDf85IvQyWXlw</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Wang, Minshi</creator><creator>Pestov, Dimitri G</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay</title><author>Wang, Minshi ; Pestov, Dimitri G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-ea82de29e13fc6cbb475ae6e0ae14db559ce9ad792682a154847f368ce0359253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Exoribonucleases - antagonists & inhibitors</topic><topic>Exoribonucleases - physiology</topic><topic>Mice</topic><topic>NIH 3T3 Cells</topic><topic>Nuclear Proteins - physiology</topic><topic>RNA</topic><topic>RNA Precursors - metabolism</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA Stability</topic><topic>RNA, Ribosomal - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Minshi</creatorcontrib><creatorcontrib>Pestov, Dimitri G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Minshi</au><au>Pestov, Dimitri G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>39</volume><issue>5</issue><spage>1811</spage><epage>1822</epage><pages>1811-1822</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Ribosome biogenesis requires multiple nuclease activities to process pre-rRNA transcripts into mature rRNA species and eliminate defective products of transcription and processing. We find that in mammalian cells, the 5' exonuclease Xrn2 plays a major role in both maturation of rRNA and degradation of a variety of discarded pre-rRNA species. Precursors of 5.8S and 28S rRNAs containing 5' extensions accumulate in mouse cells after siRNA-mediated knockdown of Xrn2, indicating similarity in the 5'-end maturation mechanisms between mammals and yeast. Strikingly, degradation of many aberrant pre-rRNA species, attributed mainly to 3' exonucleases in yeast studies, occurs 5' to 3' in mammalian cells and is mediated by Xrn2. Furthermore, depletion of Xrn2 reveals pre-rRNAs derived by cleavage events that deviate from the main processing pathway. We propose that probing of pre-rRNA maturation intermediates by exonucleases serves the dual function of generating mature rRNAs and suppressing suboptimal processing paths during ribosome assembly.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>21036871</pmid><doi>10.1093/nar/gkq1050</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Oxford Journals Open Access Collection; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Exoribonucleases - antagonists & inhibitors Exoribonucleases - physiology Mice NIH 3T3 Cells Nuclear Proteins - physiology RNA RNA Precursors - metabolism RNA Processing, Post-Transcriptional RNA Stability RNA, Ribosomal - metabolism |
| title | 5'-end surveillance by Xrn2 acts as a shared mechanism for mammalian pre-rRNA maturation and decay |
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