The human c-Kirsten ras gene is activated by a novel mutation in codon 13 in the breast carcinoma cell line MDA-MB231
We have detected amplified human Ki-ras sequences in tumorigenic NIH 3T3 cells transfected with genomic DNA from the human breast carcinoma cell line MDA-MB231. Hybridization of synthetic oligonucleotides specific for human Ki-ras sequences showed a mutation at codon 13. The polymerase chain reactio...
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| Veröffentlicht in: | Nucleic acids research 1987-08, Vol.15 (15), p.5963-5971 |
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| container_end_page | 5971 |
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| container_issue | 15 |
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| container_title | Nucleic acids research |
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| creator | KOZMA, S. C BOGAARD, M. E BUSER, K SAURER, S. M BOS, J. L GRONER, B HYNES, N. E |
| description | We have detected amplified human Ki-ras sequences in tumorigenic NIH 3T3 cells transfected with genomic DNA from the human breast carcinoma cell line MDA-MB231. Hybridization of synthetic oligonucleotides specific for human Ki-ras sequences showed a mutation at codon 13. The polymerase chain reaction with Ki-ras specific amplimers revealed a guanosine to adenosine transition at the second position of codon 13, resulting in a substitution of glycine by aspartic acid. The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line. |
| doi_str_mv | 10.1093/nar/15.15.5963 |
| format | Article |
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The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/15.15.5963</identifier><identifier>PMID: 3627975</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Breast Neoplasms - genetics ; Cell Line ; Codon ; Female ; Fundamental and applied biological sciences. 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The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - genetics</subject><subject>Cell Line</subject><subject>Codon</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Proto-Oncogenes</subject><subject>RNA, Messenger</subject><subject>Transfection</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRCoLIUrNyQfELdsbY_tJAcObaGAaMWlnK2J43SNEqfYzkr99zjqalVOlZ7kkd6HZvwIec_ZlrMWzgLGM662BarV8IJsOGhRyVaLl2TDgKmKM9m8Jm9S-sMYl1zJE3JSNHVbqw1ZbneO7pYJA7XVTx9TdoFGTPTOBUd9omiz32N2Pe0eKNIw791IpyVj9nOgvtjmvgwc1jmXsC46TJlajNaHeUJq3TjS0Ze4my_n1c2FAP6WvBpwTO7d4T0lv6--3l5-r65_fftxeX5dWQkqVxxtywCGxqpeORQaVc970Nxa4BoGK4SUfd1p3aBE7JwUXYGVDYPG6hZOyefH3Pulm1xvXcgRR3Mf_YTxwczozf9M8DtzN-8NMM00L_5PB3-c_y4uZTP5tN6Dwc1LMnWtGybheSEvfQhoxfNC2QjJ23X17aPQxjml6Ibj1pyZtXlTmjdcrVibL4YPT289yg9VF_7jgcdkcRwiBuvTUVaXTxPQwD81pLYI</recordid><startdate>19870811</startdate><enddate>19870811</enddate><creator>KOZMA, S. 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The codon 13 mutation is also detected in one Ki-ras allele of the MDA-MB231 cell line.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>3627975</pmid><doi>10.1093/nar/15.15.5963</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Animals Base Sequence Biological and medical sciences Breast Neoplasms - genetics Cell Line Codon Female Fundamental and applied biological sciences. Psychology Gene Amplification Gene expression Humans Mice Molecular and cellular biology Molecular genetics Mutation Proto-Oncogenes RNA, Messenger Transfection |
| title | The human c-Kirsten ras gene is activated by a novel mutation in codon 13 in the breast carcinoma cell line MDA-MB231 |
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