Beyond natural antibodies: the power of in vitro display technologies
In vitro display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies deriv...
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| Veröffentlicht in: | Nature biotechnology 2011-03, Vol.29 (3), p.245-254 |
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| creator | Bradbury, Andrew R M Sidhu, Sachdev Dübel, Stefan McCafferty, John |
| description | In vitro
display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using
in vitro
display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using
in vitro
display methods are now in the clinic, with many more waiting in the wings. Unlike immunization,
in vitro
display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of
in vitro
display to high-throughput applications broadens the prospects for their wider use in basic and applied research. |
| doi_str_mv | 10.1038/nbt.1791 |
| format | Article |
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display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using
in vitro
display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using
in vitro
display methods are now in the clinic, with many more waiting in the wings. Unlike immunization,
in vitro
display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of
in vitro
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display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using
in vitro
display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using
in vitro
display methods are now in the clinic, with many more waiting in the wings. Unlike immunization,
in vitro
display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of
in vitro
display to high-throughput applications broadens the prospects for their wider use in basic and applied research.</description><subject>631/1647/1511</subject><subject>631/1647/664/2228</subject><subject>631/61/24</subject><subject>Agriculture</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedical research</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Biotechnology - trends</subject><subject>Drug Design</subject><subject>Fundamental and applied biological sciences. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bradbury, Andrew R M</au><au>Sidhu, Sachdev</au><au>Dübel, Stefan</au><au>McCafferty, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Beyond natural antibodies: the power of in vitro display technologies</atitle><jtitle>Nature biotechnology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Nat Biotechnol</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>29</volume><issue>3</issue><spage>245</spage><epage>254</epage><pages>245-254</pages><issn>1087-0156</issn><issn>1546-1696</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>In vitro
display technologies, best exemplified by phage and yeast display, were first described for the selection of antibodies some 20 years ago. Since then, many antibodies have been selected and improved upon using these methods. Although it is not widely recognized, many of the antibodies derived using
in vitro
display methods have properties that would be extremely difficult, if not impossible, to obtain by immunizing animals. The first antibodies derived using
in vitro
display methods are now in the clinic, with many more waiting in the wings. Unlike immunization,
in vitro
display permits the use of defined selection conditions and provides immediate availability of the sequence encoding the antibody. The amenability of
in vitro
display to high-throughput applications broadens the prospects for their wider use in basic and applied research.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>21390033</pmid><doi>10.1038/nbt.1791</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/1511 631/1647/664/2228 631/61/24 Agriculture Antibodies Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - metabolism Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedical research Biomedicine Biotechnology Biotechnology - trends Drug Design Fundamental and applied biological sciences. Psychology Health aspects Immunization Life Sciences Monoclonal antibodies Peptide Library perspective Physiological aspects Viral antibodies Yeasts |
| title | Beyond natural antibodies: the power of in vitro display technologies |
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