The effect of the repeated subcultures of Helicobacter pylori on adhesion, motility, cytotoxicity, and gastric inflammation
In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64 times on agar plat...
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| Veröffentlicht in: | Journal of Korean medical science 2002-06, Vol.17 (3), p.302-306 |
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| creator | Kim, Sung Soo Lee, Hak Sung Cho, Young Seok Lee, Youn Soo Bhang, Choon Sang Chae, Hiun Suk Han, Sok Won Chung, In Sik Park, Doo Ho |
| description | In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64 times on agar plates containing Brucella broth and 5% bovine calf serum. The adhesion, motility, cytotoxicity, and gastric inflammation produced in Mongolian gerbils were compared between the first and 64th subcultured strain. The adhesion rates, following 3 hr exposure of AGS cells to either the first strain or the 64th-transferred strain, were 21% and 12%, respectively. The motility of the 64th-transferred strain decreased significantly when compared to the 1st strain (9.1 mm vs. 15.1 mm). The cytotoxicity index tended to be higher in the first strain than in the 64th-transferred strain (73.7% vs. 69.2%). The initial infection rate on the gerbils showed no difference between the two strains. However, chronic gastric inflammation of the first strain-infected gerbils was somewhat more severe than that of the 64th-transferred strain-infected gerbils. Therefore, the use of repeatedly subcultured strains of H. pylori in virulence experiments can lead to different results from those of the original strain. |
| doi_str_mv | 10.3346/jkms.2002.17.3.302 |
| format | Article |
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This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64 times on agar plates containing Brucella broth and 5% bovine calf serum. The adhesion, motility, cytotoxicity, and gastric inflammation produced in Mongolian gerbils were compared between the first and 64th subcultured strain. The adhesion rates, following 3 hr exposure of AGS cells to either the first strain or the 64th-transferred strain, were 21% and 12%, respectively. The motility of the 64th-transferred strain decreased significantly when compared to the 1st strain (9.1 mm vs. 15.1 mm). The cytotoxicity index tended to be higher in the first strain than in the 64th-transferred strain (73.7% vs. 69.2%). The initial infection rate on the gerbils showed no difference between the two strains. However, chronic gastric inflammation of the first strain-infected gerbils was somewhat more severe than that of the 64th-transferred strain-infected gerbils. Therefore, the use of repeatedly subcultured strains of H. pylori in virulence experiments can lead to different results from those of the original strain.</description><identifier>ISSN: 1011-8934</identifier><identifier>EISSN: 1598-6357</identifier><identifier>DOI: 10.3346/jkms.2002.17.3.302</identifier><identifier>PMID: 12068131</identifier><language>eng</language><publisher>Korea (South): Korean Academy of Medical Sciences</publisher><subject>Animals ; Bacterial Adhesion ; Gastritis - immunology ; Gastritis - microbiology ; Gerbillinae ; Helicobacter Infections - immunology ; Helicobacter Infections - microbiology ; Helicobacter pylori - growth & development ; Helicobacter pylori - pathogenicity ; Male ; Virulence</subject><ispartof>Journal of Korean medical science, 2002-06, Vol.17 (3), p.302-306</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-892f314ec101d36ab9e6b37ec7f10f81a57c3ecb399bbb55971a920eba2835403</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054887/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054887/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12068131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sung Soo</creatorcontrib><creatorcontrib>Lee, Hak Sung</creatorcontrib><creatorcontrib>Cho, Young Seok</creatorcontrib><creatorcontrib>Lee, Youn Soo</creatorcontrib><creatorcontrib>Bhang, Choon Sang</creatorcontrib><creatorcontrib>Chae, Hiun Suk</creatorcontrib><creatorcontrib>Han, Sok Won</creatorcontrib><creatorcontrib>Chung, In Sik</creatorcontrib><creatorcontrib>Park, Doo Ho</creatorcontrib><title>The effect of the repeated subcultures of Helicobacter pylori on adhesion, motility, cytotoxicity, and gastric inflammation</title><title>Journal of Korean medical science</title><addtitle>J Korean Med Sci</addtitle><description>In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64 times on agar plates containing Brucella broth and 5% bovine calf serum. The adhesion, motility, cytotoxicity, and gastric inflammation produced in Mongolian gerbils were compared between the first and 64th subcultured strain. The adhesion rates, following 3 hr exposure of AGS cells to either the first strain or the 64th-transferred strain, were 21% and 12%, respectively. The motility of the 64th-transferred strain decreased significantly when compared to the 1st strain (9.1 mm vs. 15.1 mm). The cytotoxicity index tended to be higher in the first strain than in the 64th-transferred strain (73.7% vs. 69.2%). The initial infection rate on the gerbils showed no difference between the two strains. However, chronic gastric inflammation of the first strain-infected gerbils was somewhat more severe than that of the 64th-transferred strain-infected gerbils. Therefore, the use of repeatedly subcultured strains of H. pylori in virulence experiments can lead to different results from those of the original strain.</description><subject>Animals</subject><subject>Bacterial Adhesion</subject><subject>Gastritis - immunology</subject><subject>Gastritis - microbiology</subject><subject>Gerbillinae</subject><subject>Helicobacter Infections - immunology</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori - growth & development</subject><subject>Helicobacter pylori - pathogenicity</subject><subject>Male</subject><subject>Virulence</subject><issn>1011-8934</issn><issn>1598-6357</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtv1DAQthAVLYU_wAH5xKkb_Ijj-IKEqkKRKnFpz5btjLsuTrzYDmLVP4-XrnicZkbzPWb0IfSGko7zfnj_8G0uHSOEdVR2vOOEPUNnVKhxM3Ahn7eeULoZFe9P0ctSHhpSCMZfoFPKyDBSTs_Q4-0WMHgPruLkcW1Thh2YChMuq3VrrGuGcthdQwwuWeMqZLzbx5QDTgs20xZKSMsFnlMNMdT9BXb7mmr6GdzvySwTvjel5uBwWHw082xqY7xCJ97EAq-P9Rzdfbq6vbze3Hz9_OXy483G9UNf2wPMc9qDa-9MfDBWwWC5BCc9JX6kRkjHwVmulLVWCCWpUYyANWzkoif8HH140t2tdobJwVKziXqXw2zyXicT9P-bJWz1ffqhORH9OMom8O4okNP3FUrVcygOYjQLpLVoSUemCFMNyJ6ALqdSMvg_JpToQ2b6kJk-ZKap1Lw5sEZ6--95fynHkPgv_Q2XWQ</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Kim, Sung Soo</creator><creator>Lee, Hak Sung</creator><creator>Cho, Young Seok</creator><creator>Lee, Youn Soo</creator><creator>Bhang, Choon Sang</creator><creator>Chae, Hiun Suk</creator><creator>Han, Sok Won</creator><creator>Chung, In Sik</creator><creator>Park, Doo Ho</creator><general>Korean Academy of Medical Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020601</creationdate><title>The effect of the repeated subcultures of Helicobacter pylori on adhesion, motility, cytotoxicity, and gastric inflammation</title><author>Kim, Sung Soo ; Lee, Hak Sung ; Cho, Young Seok ; Lee, Youn Soo ; Bhang, Choon Sang ; Chae, Hiun Suk ; Han, Sok Won ; Chung, In Sik ; Park, Doo Ho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-892f314ec101d36ab9e6b37ec7f10f81a57c3ecb399bbb55971a920eba2835403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Bacterial Adhesion</topic><topic>Gastritis - immunology</topic><topic>Gastritis - microbiology</topic><topic>Gerbillinae</topic><topic>Helicobacter Infections - immunology</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori - growth & development</topic><topic>Helicobacter pylori - pathogenicity</topic><topic>Male</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sung Soo</creatorcontrib><creatorcontrib>Lee, Hak Sung</creatorcontrib><creatorcontrib>Cho, Young Seok</creatorcontrib><creatorcontrib>Lee, Youn Soo</creatorcontrib><creatorcontrib>Bhang, Choon Sang</creatorcontrib><creatorcontrib>Chae, Hiun Suk</creatorcontrib><creatorcontrib>Han, Sok Won</creatorcontrib><creatorcontrib>Chung, In Sik</creatorcontrib><creatorcontrib>Park, Doo Ho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Korean medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sung Soo</au><au>Lee, Hak Sung</au><au>Cho, Young Seok</au><au>Lee, Youn Soo</au><au>Bhang, Choon Sang</au><au>Chae, Hiun Suk</au><au>Han, Sok Won</au><au>Chung, In Sik</au><au>Park, Doo Ho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of the repeated subcultures of Helicobacter pylori on adhesion, motility, cytotoxicity, and gastric inflammation</atitle><jtitle>Journal of Korean medical science</jtitle><addtitle>J Korean Med Sci</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>17</volume><issue>3</issue><spage>302</spage><epage>306</epage><pages>302-306</pages><issn>1011-8934</issn><eissn>1598-6357</eissn><abstract>In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64 times on agar plates containing Brucella broth and 5% bovine calf serum. The adhesion, motility, cytotoxicity, and gastric inflammation produced in Mongolian gerbils were compared between the first and 64th subcultured strain. The adhesion rates, following 3 hr exposure of AGS cells to either the first strain or the 64th-transferred strain, were 21% and 12%, respectively. The motility of the 64th-transferred strain decreased significantly when compared to the 1st strain (9.1 mm vs. 15.1 mm). The cytotoxicity index tended to be higher in the first strain than in the 64th-transferred strain (73.7% vs. 69.2%). The initial infection rate on the gerbils showed no difference between the two strains. However, chronic gastric inflammation of the first strain-infected gerbils was somewhat more severe than that of the 64th-transferred strain-infected gerbils. Therefore, the use of repeatedly subcultured strains of H. pylori in virulence experiments can lead to different results from those of the original strain.</abstract><cop>Korea (South)</cop><pub>Korean Academy of Medical Sciences</pub><pmid>12068131</pmid><doi>10.3346/jkms.2002.17.3.302</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; PubMed Central Open Access; KoreaMed Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Bacterial Adhesion Gastritis - immunology Gastritis - microbiology Gerbillinae Helicobacter Infections - immunology Helicobacter Infections - microbiology Helicobacter pylori - growth & development Helicobacter pylori - pathogenicity Male Virulence |
| title | The effect of the repeated subcultures of Helicobacter pylori on adhesion, motility, cytotoxicity, and gastric inflammation |
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