Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation
During embryonic development and embryonic stem cell (ESC) differentiation, the different cell lineages of the mature heart arise from two types of multipotent cardiovascular progenitors (MCPs), the first and second heart fields. A key question is whether these two MCP populations arise from differe...
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| Veröffentlicht in: | The Journal of cell biology 2011-03, Vol.192 (5), p.751-765 |
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| container_issue | 5 |
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| container_title | The Journal of cell biology |
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| creator | Bondue, Antoine Tännler, Simon Chiapparo, Giuseppe Chabab, Samira Ramialison, Mirana Paulissen, Catherine Beck, Benjamin Harvey, Richard Blanpain, Cédric |
| description | During embryonic development and embryonic stem cell (ESC) differentiation, the different cell lineages of the mature heart arise from two types of multipotent cardiovascular progenitors (MCPs), the first and second heart fields. A key question is whether these two MCP populations arise from differentiation of a common progenitor. In this paper, we engineered Mesp1-green fluorescent protein (GFP) ESCs to isolate early MCPs during ESC differentiation. Mesp1-GFP cells are strongly enriched for MCPs, presenting the ability to differentiate into multiple cardiovascular lineages from both heart fields in vitro and in vivo. Transcriptional profiling of Mesp1-GFP cells uncovered cell surface markers expressed by MCPs allowing their prospective isolation. Mesp1 is required for MCP specification and the expression of key cardiovascular transcription factors. Isl1 is expressed in a subset of early Mesp1-expressing cells independently of Mesp1 and acts together with Mesp1 to promote cardiovascular differentiation. Our study identifies the early MCPs residing at the top of the cellular hierarchy of cardiovascular lineages during ESC differentiation. |
| doi_str_mv | 10.1083/jcb.201007063 |
| format | Article |
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A key question is whether these two MCP populations arise from differentiation of a common progenitor. In this paper, we engineered Mesp1-green fluorescent protein (GFP) ESCs to isolate early MCPs during ESC differentiation. Mesp1-GFP cells are strongly enriched for MCPs, presenting the ability to differentiate into multiple cardiovascular lineages from both heart fields in vitro and in vivo. Transcriptional profiling of Mesp1-GFP cells uncovered cell surface markers expressed by MCPs allowing their prospective isolation. Mesp1 is required for MCP specification and the expression of key cardiovascular transcription factors. Isl1 is expressed in a subset of early Mesp1-expressing cells independently of Mesp1 and acts together with Mesp1 to promote cardiovascular differentiation. Our study identifies the early MCPs residing at the top of the cellular hierarchy of cardiovascular lineages during ESC differentiation.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.201007063</identifier><identifier>PMID: 21383076</identifier><identifier>CODEN: JCLBA3</identifier><language>eng</language><publisher>United States: The Rockefeller University Press</publisher><subject>Animals ; Basic Helix-Loop-Helix Transcription Factors - analysis ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Basic Helix-Loop-Helix Transcription Factors - physiology ; Cardiovascular system ; Cell Differentiation ; Cell Lineage ; Embryonic Stem Cells - cytology ; Gene Expression Profiling ; Green Fluorescent Proteins - analysis ; Heart ; Heart - embryology ; Homeodomain Proteins - metabolism ; LIM-Homeodomain Proteins ; Mice ; Myocardium - cytology ; Proteins ; Recombinant Fusion Proteins - analysis ; Stem cells ; Transcription Factors</subject><ispartof>The Journal of cell biology, 2011-03, Vol.192 (5), p.751-765</ispartof><rights>Copyright Rockefeller University Press Mar 7, 2011</rights><rights>2011 Bondue et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-76e0cab2688166ea6af760615563af243e28aa8c63c09cc5dcffaebaa092dcf23</citedby><cites>FETCH-LOGICAL-c535t-76e0cab2688166ea6af760615563af243e28aa8c63c09cc5dcffaebaa092dcf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21383076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bondue, Antoine</creatorcontrib><creatorcontrib>Tännler, Simon</creatorcontrib><creatorcontrib>Chiapparo, Giuseppe</creatorcontrib><creatorcontrib>Chabab, Samira</creatorcontrib><creatorcontrib>Ramialison, Mirana</creatorcontrib><creatorcontrib>Paulissen, Catherine</creatorcontrib><creatorcontrib>Beck, Benjamin</creatorcontrib><creatorcontrib>Harvey, Richard</creatorcontrib><creatorcontrib>Blanpain, Cédric</creatorcontrib><title>Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>During embryonic development and embryonic stem cell (ESC) differentiation, the different cell lineages of the mature heart arise from two types of multipotent cardiovascular progenitors (MCPs), the first and second heart fields. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Basic Helix-Loop-Helix Transcription Factors - analysis Basic Helix-Loop-Helix Transcription Factors - genetics Basic Helix-Loop-Helix Transcription Factors - physiology Cardiovascular system Cell Differentiation Cell Lineage Embryonic Stem Cells - cytology Gene Expression Profiling Green Fluorescent Proteins - analysis Heart Heart - embryology Homeodomain Proteins - metabolism LIM-Homeodomain Proteins Mice Myocardium - cytology Proteins Recombinant Fusion Proteins - analysis Stem cells Transcription Factors |
| title | Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation |
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