Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands
The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH stretching vib...
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| Veröffentlicht in: | Journal of Biomedical Optics 2011-02, Vol.16 (2), p.021104-021104 |
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| container_title | Journal of Biomedical Optics |
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| creator | Lin, Chia-Yu Potma, Eric O Suhalim, Jeffrey L Nien, Chyong Ly Jester, James V Miljkovic, Milo D Diem, Max |
| description | The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH
stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. |
| doi_str_mv | 10.1117/1.3533716 |
| format | Article |
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stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.3533716</identifier><identifier>PMID: 21361667</identifier><identifier>CODEN: JBOPFO</identifier><language>eng</language><publisher>United States: Society of Photo-Optical Instrumentation Engineers (SPIE)</publisher><subject>Animals ; Cars ; Ducts ; Image Enhancement - methods ; Image Interpretation, Computer-Assisted - methods ; Imaging ; Lipids ; Meibomian Glands - cytology ; Mice ; Microscopy - methods ; Principal Component Analysis ; Raman scattering ; Reproducibility of Results ; Sensitivity and Specificity ; Special Section on Coherent Raman Imaging Techniques and Biomedical Applications ; Spectra ; Spectrum Analysis, Raman - methods ; Tomography, Optical Coherence - methods ; Utilities</subject><ispartof>Journal of Biomedical Optics, 2011-02, Vol.16 (2), p.021104-021104</ispartof><rights>2011 COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.</rights><rights>Copyright © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-b396827993135fec54cf286091aeeaaef4918aa1c29d82e637b00242aab398613</citedby><cites>FETCH-LOGICAL-c450t-b396827993135fec54cf286091aeeaaef4918aa1c29d82e637b00242aab398613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048879/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048879/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21361667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Chia-Yu</creatorcontrib><creatorcontrib>Potma, Eric O</creatorcontrib><creatorcontrib>Suhalim, Jeffrey L</creatorcontrib><creatorcontrib>Nien, Chyong Ly</creatorcontrib><creatorcontrib>Jester, James V</creatorcontrib><creatorcontrib>Miljkovic, Milo D</creatorcontrib><creatorcontrib>Diem, Max</creatorcontrib><title>Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands</title><title>Journal of Biomedical Optics</title><addtitle>J Biomed Opt</addtitle><description>The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH
stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.</description><subject>Animals</subject><subject>Cars</subject><subject>Ducts</subject><subject>Image Enhancement - methods</subject><subject>Image Interpretation, Computer-Assisted - methods</subject><subject>Imaging</subject><subject>Lipids</subject><subject>Meibomian Glands - cytology</subject><subject>Mice</subject><subject>Microscopy - methods</subject><subject>Principal Component Analysis</subject><subject>Raman scattering</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Special Section on Coherent Raman Imaging Techniques and Biomedical Applications</subject><subject>Spectra</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Tomography, Optical Coherence - methods</subject><subject>Utilities</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EoqVw4A8g34BDisdOHPuCBBXlQ5W24uNszXonu4YkDrGXqv8eb3dZwYWLx_I88_odvYw9BXEOAO0rOFeNUi3oe-wUGi0qKQ3cL3dhVKW0NifsUUrfhRBGW_2QnUhQGrRuT9nNdfAxkY_jiqeJfJ6x5z5uaKYxcxxzqL7k-IMS_4wDjjx5zJnmMK55GHC9qzchb_hUnnyY7oaHKY77aexvU0g8dnygsIxDKArrHsdVeswedNgnenKoZ-zb5buvFx-qq8X7jxdvripfNyJXS2W1ka21ClTTkW9q30mjhQUkQqSutmAQwUu7MpK0apdCyFoilkmjQZ2x13vdabscaOWLr7KhK3YHnG9dxOD-7Yxh49bxl1OiNqa1ReD5QWCOP7eUshtC8tSXLShukyufSCGtFoV88V8SdAu1AGV2rl7uUT_HlGbqjoZAuF2kDtwh0sI--3uDI_knwwLIPZCmQMf2p7eL68tFiVyA3p2iuAQQ9d0d1G9TVKv6</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Lin, Chia-Yu</creator><creator>Potma, Eric O</creator><creator>Suhalim, Jeffrey L</creator><creator>Nien, Chyong Ly</creator><creator>Jester, James V</creator><creator>Miljkovic, Milo D</creator><creator>Diem, Max</creator><general>Society of Photo-Optical Instrumentation Engineers (SPIE)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110201</creationdate><title>Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands</title><author>Lin, Chia-Yu ; Potma, Eric O ; Suhalim, Jeffrey L ; Nien, Chyong Ly ; Jester, James V ; Miljkovic, Milo D ; Diem, Max</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-b396827993135fec54cf286091aeeaaef4918aa1c29d82e637b00242aab398613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Cars</topic><topic>Ducts</topic><topic>Image Enhancement - methods</topic><topic>Image Interpretation, Computer-Assisted - methods</topic><topic>Imaging</topic><topic>Lipids</topic><topic>Meibomian Glands - cytology</topic><topic>Mice</topic><topic>Microscopy - methods</topic><topic>Principal Component Analysis</topic><topic>Raman scattering</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Special Section on Coherent Raman Imaging Techniques and Biomedical Applications</topic><topic>Spectra</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Tomography, Optical Coherence - methods</topic><topic>Utilities</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Chia-Yu</creatorcontrib><creatorcontrib>Potma, Eric O</creatorcontrib><creatorcontrib>Suhalim, Jeffrey L</creatorcontrib><creatorcontrib>Nien, Chyong Ly</creatorcontrib><creatorcontrib>Jester, James V</creatorcontrib><creatorcontrib>Miljkovic, Milo D</creatorcontrib><creatorcontrib>Diem, Max</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Biomedical Optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Chia-Yu</au><au>Potma, Eric O</au><au>Suhalim, Jeffrey L</au><au>Nien, Chyong Ly</au><au>Jester, James V</au><au>Miljkovic, Milo D</au><au>Diem, Max</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands</atitle><jtitle>Journal of Biomedical Optics</jtitle><addtitle>J Biomed Opt</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>16</volume><issue>2</issue><spage>021104</spage><epage>021104</epage><pages>021104-021104</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><coden>JBOPFO</coden><abstract>The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH
stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.</abstract><cop>United States</cop><pub>Society of Photo-Optical Instrumentation Engineers (SPIE)</pub><pmid>21361667</pmid><doi>10.1117/1.3533716</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Cars Ducts Image Enhancement - methods Image Interpretation, Computer-Assisted - methods Imaging Lipids Meibomian Glands - cytology Mice Microscopy - methods Principal Component Analysis Raman scattering Reproducibility of Results Sensitivity and Specificity Special Section on Coherent Raman Imaging Techniques and Biomedical Applications Spectra Spectrum Analysis, Raman - methods Tomography, Optical Coherence - methods Utilities |
| title | Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands |
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