Specific binding of endothelin on human vascular smooth muscle cells in culture

Endothelin is a newly discovered, potent vasoconstrictor peptide secreted by endothelial cells. The binding of endothelin was studied on cultured human vascular smooth muscle cells obtained from umbilical veins. A single specific binding site for 125I-endothelin was identified, with an apparent Kd o...

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Veröffentlicht in:	J. Clin. Invest.; (United States) 1989-05, Vol.83 (5), p.1758-1761
Hauptverfasser:	CLOZEL, M, FISCHLI, W, GUILLY, C
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry ANIMAL TISSUES ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES Binding, Competitive BIOCHEMICAL REACTION KINETICS BIOCHEMISTRY Biological and medical sciences BLOOD VESSELS BODY CARDIOVASCULAR AGENTS CARDIOVASCULAR SYSTEM CELL CULTURES Cells, Cultured CHEMISTRY CULTURE MEDIA DAYS LIVING RADIOISOTOPES DRUGS ELECTRON CAPTURE RADIOISOTOPES Endothelins ENDOTHELIUM Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Humans INHIBITION INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES Iodine Radioisotopes ISOTOPE APPLICATIONS ISOTOPES KINETICS MAMMALS MAN MEMBRANE PROTEINS Muscle, Smooth, Vascular - metabolism MUSCLES NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PEPTIDES Peptides - metabolism PRIMATES PROTEINS RADIOISOTOPES REACTION KINETICS RECEPTORS TISSUES TRACER TECHNIQUES Umbilical Veins VASOCONSTRICTORS VEINS VERTEBRATES
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