The Label-Free Unambiguous Detection and Symbolic Display of Single Nucleotide Polymorphisms on DNA Origami
Single nucleotide polymorphisms (SNPs) are the most common genetic variation in the human genome. Kinetic methods based on branch migration have proved successful for detecting SNPs because a mispair inhibits the progress of branch migration in the direction of the mispair. We have combined the effe...
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| Veröffentlicht in: | Nano letters 2011-02, Vol.11 (2), p.910-913 |
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| creator | Subramanian, Hari K. K Chakraborty, Banani Sha, Ruojie Seeman, Nadrian C |
| description | Single nucleotide polymorphisms (SNPs) are the most common genetic variation in the human genome. Kinetic methods based on branch migration have proved successful for detecting SNPs because a mispair inhibits the progress of branch migration in the direction of the mispair. We have combined the effectiveness of kinetic methods with atomic force microscopy of DNA origami patterns to produce a direct visual readout of the target nucleotide contained in the probe sequence. The origami contains graphical representations of the four nucleotide alphabetic characters, A, T, G and C, and the symbol containing the test nucleotide identity vanishes in the presence of the probe. The system also works with pairs of probes, corresponding to heterozygous diploid genomes. |
| doi_str_mv | 10.1021/nl104555t |
| format | Article |
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The origami contains graphical representations of the four nucleotide alphabetic characters, A, T, G and C, and the symbol containing the test nucleotide identity vanishes in the presence of the probe. 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We have combined the effectiveness of kinetic methods with atomic force microscopy of DNA origami patterns to produce a direct visual readout of the target nucleotide contained in the probe sequence. The origami contains graphical representations of the four nucleotide alphabetic characters, A, T, G and C, and the symbol containing the test nucleotide identity vanishes in the presence of the probe. The system also works with pairs of probes, corresponding to heterozygous diploid genomes.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21235216</pmid><doi>10.1021/nl104555t</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Condensed matter: structure, mechanical and thermal properties Diffusion in nanoscale solids Diffusion in solids DNA - analysis DNA - genetics DNA Mutational Analysis - methods Exact sciences and technology Microscopy, Atomic Force - methods Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals Nanotechnology - methods Physics Polymorphism, Single Nucleotide - genetics Sequence Analysis, DNA - methods Staining and Labeling Structure of solids and liquids crystallography Transport properties of condensed matter (nonelectronic) |
| title | The Label-Free Unambiguous Detection and Symbolic Display of Single Nucleotide Polymorphisms on DNA Origami |
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