Induction of immune memory by a multisubunit chlamydial vaccine
Abstract We tested the hypothesis that intramuscular immunization with a multisubunit chlamydial vaccine candidate will induce long lasting immune responses in mice. Accordingly, groups of female C57BL/6 mice were immunized intramuscularly with Vibrio cholerae ghosts (VCG) expressing the Poring B an...
Gespeichert in:
| Veröffentlicht in: | Vaccine 2011-02, Vol.29 (7), p.1472-1480 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1480 |
|---|---|
| container_issue | 7 |
| container_start_page | 1472 |
| container_title | Vaccine |
| container_volume | 29 |
| creator | Eko, F.O Ekong, E He, Q Black, C.M Igietseme, J.U |
| description | Abstract We tested the hypothesis that intramuscular immunization with a multisubunit chlamydial vaccine candidate will induce long lasting immune responses in mice. Accordingly, groups of female C57BL/6 mice were immunized intramuscularly with Vibrio cholerae ghosts (VCG) expressing the Poring B and polymorphic membrane protein-D proteins of Chlamydia trachomatis or a control antigen. Humoral and cell-mediated immune responses were evaluated following immunization and after live chlamydial infection. Immunization induced an anamnestic response characterized by chlamydial-specific IgG2a and IgA antibodies in sera and vaginal lavage as well as specific genital and splenic T cell responses. The results also revealed that the local mucosal and systemic cellular and humoral immune effectors induced in mice following immunization with the vaccine candidate are long lasting. Vaccinated mice cleared intravaginal challenge with 105 chlamydial inclusion forming units within 12 days compared to control mice, which shed up to 2 × 103 IFUs at this time point. Moreover, rechallenge of mice 98 days after resolution of the primary infection resulted in the recall and retention of a relatively high frequency of chlamydial-specific Th1 cells and IgG2a in the genital mucosa. These results provide the first evidence that a VCG-based multisubunit chlamydial vaccine is capable of effectively stimulating anamnestic systemic and mucosal immune responses in mice. The data support further vaccine evaluation and testing for induction of long-term protective immunity. |
| doi_str_mv | 10.1016/j.vaccine.2010.12.024 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3032637</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X10017627</els_id><sourcerecordid>3218575421</sourcerecordid><originalsourceid>FETCH-LOGICAL-c635t-964733f005f5cd913127b29ab98c00596c7accecbdae317aa7a784eb70e4b4d13</originalsourceid><addsrcrecordid>eNqFkk9v1DAQxS0EotuFjwBEQhWnXTy2EzuXVqjiT6VKHEolbpbjTFoviV3sZKX99jjapYVeerI0_s3z87wh5A3QNVCoPm7WW2Ot87hmdK6xNWXiGVmAknzFSlDPyYKySqwE0J9H5DilDaW05FC_JEcMQAlVqgU5u_DtZEcXfBG6wg3D5LEYcAhxVzS7whTD1I8uTc3k3VjY294Mu9aZvji8_oq86Eyf8PXhXJLrL59_nH9bXX7_enH-6XJlK16Oq7oSkvMuG-hK29bAgcmG1aaplc3FurIy66FtWoMcpDHSSCWwkRRFI1rgS3K6172bmgFbi36Mptd30Q0m7nQwTv9_492tvglbzSlnFZdZ4MNBIIbfE6ZRDy5Z7HvjMUxJq0rUpVISniaFqpSo8yyX5P0jchOm6PMcNIhaAdAKVKbKPWVjSClid-8aqJ6z1Bt9mKaes9TAdM4y973998v3XX_Dy8DJATDJmr6LxluXHjiePZaqzty7PdeZoM1NzMz1VX6ppBS4knJWOtsTmCPcOow6WYfeYusi2lG3wT1p9vSRgu2dd9nWL9xhepiLTrlBX82rOW8mZA-yYpL_AaId3aE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1498110618</pqid></control><display><type>article</type><title>Induction of immune memory by a multisubunit chlamydial vaccine</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><source>ProQuest Central UK/Ireland</source><creator>Eko, F.O ; Ekong, E ; He, Q ; Black, C.M ; Igietseme, J.U</creator><creatorcontrib>Eko, F.O ; Ekong, E ; He, Q ; Black, C.M ; Igietseme, J.U</creatorcontrib><description>Abstract We tested the hypothesis that intramuscular immunization with a multisubunit chlamydial vaccine candidate will induce long lasting immune responses in mice. Accordingly, groups of female C57BL/6 mice were immunized intramuscularly with Vibrio cholerae ghosts (VCG) expressing the Poring B and polymorphic membrane protein-D proteins of Chlamydia trachomatis or a control antigen. Humoral and cell-mediated immune responses were evaluated following immunization and after live chlamydial infection. Immunization induced an anamnestic response characterized by chlamydial-specific IgG2a and IgA antibodies in sera and vaginal lavage as well as specific genital and splenic T cell responses. The results also revealed that the local mucosal and systemic cellular and humoral immune effectors induced in mice following immunization with the vaccine candidate are long lasting. Vaccinated mice cleared intravaginal challenge with 105 chlamydial inclusion forming units within 12 days compared to control mice, which shed up to 2 × 103 IFUs at this time point. Moreover, rechallenge of mice 98 days after resolution of the primary infection resulted in the recall and retention of a relatively high frequency of chlamydial-specific Th1 cells and IgG2a in the genital mucosa. These results provide the first evidence that a VCG-based multisubunit chlamydial vaccine is capable of effectively stimulating anamnestic systemic and mucosal immune responses in mice. The data support further vaccine evaluation and testing for induction of long-term protective immunity.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.12.024</identifier><identifier>PMID: 21184858</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; antibodies ; Antibodies, Bacterial - blood ; antigens ; Applied microbiology ; Bacterial Proteins - immunology ; Bacterial Vaccines - immunology ; Bacteriology ; Biological and medical sciences ; Cell Proliferation ; cell-mediated immunity ; Chlamydia ; Chlamydia Infections - immunology ; Chlamydia Infections - prevention & control ; Chlamydia trachomatis ; Chlamydia trachomatis - immunology ; Delivery ; Female ; Fundamental and applied biological sciences. Psychology ; immune response ; Immune system ; Immunity ; Immunity, Cellular ; Immunity, Humoral ; Immunization ; immunoglobulin A ; Immunoglobulin A - blood ; Immunoglobulin A - immunology ; immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Immunologic Memory ; Infections ; Injections, Intramuscular ; Membrane Proteins - immunology ; Mice ; Mice, Inbred C57BL ; Microbiology ; Miscellaneous ; mucosa ; mucosal immunity ; Porins - immunology ; Sexually transmitted diseases ; Spleen - immunology ; STD ; T-lymphocytes ; Th1 Cells - immunology ; Vaccine ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vagina - immunology ; Vibrio cholerae ; Vibrio cholerae - immunology ; Virus Shedding</subject><ispartof>Vaccine, 2011-02, Vol.29 (7), p.1472-1480</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Feb 4, 2011</rights><rights>2010 Elsevier Ltd. All rights reserved. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c635t-964733f005f5cd913127b29ab98c00596c7accecbdae317aa7a784eb70e4b4d13</citedby><cites>FETCH-LOGICAL-c635t-964733f005f5cd913127b29ab98c00596c7accecbdae317aa7a784eb70e4b4d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1498110618?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23905589$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21184858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eko, F.O</creatorcontrib><creatorcontrib>Ekong, E</creatorcontrib><creatorcontrib>He, Q</creatorcontrib><creatorcontrib>Black, C.M</creatorcontrib><creatorcontrib>Igietseme, J.U</creatorcontrib><title>Induction of immune memory by a multisubunit chlamydial vaccine</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract We tested the hypothesis that intramuscular immunization with a multisubunit chlamydial vaccine candidate will induce long lasting immune responses in mice. Accordingly, groups of female C57BL/6 mice were immunized intramuscularly with Vibrio cholerae ghosts (VCG) expressing the Poring B and polymorphic membrane protein-D proteins of Chlamydia trachomatis or a control antigen. Humoral and cell-mediated immune responses were evaluated following immunization and after live chlamydial infection. Immunization induced an anamnestic response characterized by chlamydial-specific IgG2a and IgA antibodies in sera and vaginal lavage as well as specific genital and splenic T cell responses. The results also revealed that the local mucosal and systemic cellular and humoral immune effectors induced in mice following immunization with the vaccine candidate are long lasting. Vaccinated mice cleared intravaginal challenge with 105 chlamydial inclusion forming units within 12 days compared to control mice, which shed up to 2 × 103 IFUs at this time point. Moreover, rechallenge of mice 98 days after resolution of the primary infection resulted in the recall and retention of a relatively high frequency of chlamydial-specific Th1 cells and IgG2a in the genital mucosa. These results provide the first evidence that a VCG-based multisubunit chlamydial vaccine is capable of effectively stimulating anamnestic systemic and mucosal immune responses in mice. The data support further vaccine evaluation and testing for induction of long-term protective immunity.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>antigens</subject><subject>Applied microbiology</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>cell-mediated immunity</subject><subject>Chlamydia</subject><subject>Chlamydia Infections - immunology</subject><subject>Chlamydia Infections - prevention & control</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - immunology</subject><subject>Delivery</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>immune response</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunity, Cellular</subject><subject>Immunity, Humoral</subject><subject>Immunization</subject><subject>immunoglobulin A</subject><subject>Immunoglobulin A - blood</subject><subject>Immunoglobulin A - immunology</subject><subject>immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunologic Memory</subject><subject>Infections</subject><subject>Injections, Intramuscular</subject><subject>Membrane Proteins - immunology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>mucosa</subject><subject>mucosal immunity</subject><subject>Porins - immunology</subject><subject>Sexually transmitted diseases</subject><subject>Spleen - immunology</subject><subject>STD</subject><subject>T-lymphocytes</subject><subject>Th1 Cells - immunology</subject><subject>Vaccine</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vagina - immunology</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - immunology</subject><subject>Virus Shedding</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkk9v1DAQxS0EotuFjwBEQhWnXTy2EzuXVqjiT6VKHEolbpbjTFoviV3sZKX99jjapYVeerI0_s3z87wh5A3QNVCoPm7WW2Ot87hmdK6xNWXiGVmAknzFSlDPyYKySqwE0J9H5DilDaW05FC_JEcMQAlVqgU5u_DtZEcXfBG6wg3D5LEYcAhxVzS7whTD1I8uTc3k3VjY294Mu9aZvji8_oq86Eyf8PXhXJLrL59_nH9bXX7_enH-6XJlK16Oq7oSkvMuG-hK29bAgcmG1aaplc3FurIy66FtWoMcpDHSSCWwkRRFI1rgS3K6172bmgFbi36Mptd30Q0m7nQwTv9_492tvglbzSlnFZdZ4MNBIIbfE6ZRDy5Z7HvjMUxJq0rUpVISniaFqpSo8yyX5P0jchOm6PMcNIhaAdAKVKbKPWVjSClid-8aqJ6z1Bt9mKaes9TAdM4y973998v3XX_Dy8DJATDJmr6LxluXHjiePZaqzty7PdeZoM1NzMz1VX6ppBS4knJWOtsTmCPcOow6WYfeYusi2lG3wT1p9vSRgu2dd9nWL9xhepiLTrlBX82rOW8mZA-yYpL_AaId3aE</recordid><startdate>20110204</startdate><enddate>20110204</enddate><creator>Eko, F.O</creator><creator>Ekong, E</creator><creator>He, Q</creator><creator>Black, C.M</creator><creator>Igietseme, J.U</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20110204</creationdate><title>Induction of immune memory by a multisubunit chlamydial vaccine</title><author>Eko, F.O ; Ekong, E ; He, Q ; Black, C.M ; Igietseme, J.U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c635t-964733f005f5cd913127b29ab98c00596c7accecbdae317aa7a784eb70e4b4d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>antigens</topic><topic>Applied microbiology</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>cell-mediated immunity</topic><topic>Chlamydia</topic><topic>Chlamydia Infections - immunology</topic><topic>Chlamydia Infections - prevention & control</topic><topic>Chlamydia trachomatis</topic><topic>Chlamydia trachomatis - immunology</topic><topic>Delivery</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>immune response</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunity, Cellular</topic><topic>Immunity, Humoral</topic><topic>Immunization</topic><topic>immunoglobulin A</topic><topic>Immunoglobulin A - blood</topic><topic>Immunoglobulin A - immunology</topic><topic>immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunologic Memory</topic><topic>Infections</topic><topic>Injections, Intramuscular</topic><topic>Membrane Proteins - immunology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>mucosa</topic><topic>mucosal immunity</topic><topic>Porins - immunology</topic><topic>Sexually transmitted diseases</topic><topic>Spleen - immunology</topic><topic>STD</topic><topic>T-lymphocytes</topic><topic>Th1 Cells - immunology</topic><topic>Vaccine</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vagina - immunology</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - immunology</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eko, F.O</creatorcontrib><creatorcontrib>Ekong, E</creatorcontrib><creatorcontrib>He, Q</creatorcontrib><creatorcontrib>Black, C.M</creatorcontrib><creatorcontrib>Igietseme, J.U</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eko, F.O</au><au>Ekong, E</au><au>He, Q</au><au>Black, C.M</au><au>Igietseme, J.U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of immune memory by a multisubunit chlamydial vaccine</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2011-02-04</date><risdate>2011</risdate><volume>29</volume><issue>7</issue><spage>1472</spage><epage>1480</epage><pages>1472-1480</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract We tested the hypothesis that intramuscular immunization with a multisubunit chlamydial vaccine candidate will induce long lasting immune responses in mice. Accordingly, groups of female C57BL/6 mice were immunized intramuscularly with Vibrio cholerae ghosts (VCG) expressing the Poring B and polymorphic membrane protein-D proteins of Chlamydia trachomatis or a control antigen. Humoral and cell-mediated immune responses were evaluated following immunization and after live chlamydial infection. Immunization induced an anamnestic response characterized by chlamydial-specific IgG2a and IgA antibodies in sera and vaginal lavage as well as specific genital and splenic T cell responses. The results also revealed that the local mucosal and systemic cellular and humoral immune effectors induced in mice following immunization with the vaccine candidate are long lasting. Vaccinated mice cleared intravaginal challenge with 105 chlamydial inclusion forming units within 12 days compared to control mice, which shed up to 2 × 103 IFUs at this time point. Moreover, rechallenge of mice 98 days after resolution of the primary infection resulted in the recall and retention of a relatively high frequency of chlamydial-specific Th1 cells and IgG2a in the genital mucosa. These results provide the first evidence that a VCG-based multisubunit chlamydial vaccine is capable of effectively stimulating anamnestic systemic and mucosal immune responses in mice. The data support further vaccine evaluation and testing for induction of long-term protective immunity.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21184858</pmid><doi>10.1016/j.vaccine.2010.12.024</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2011-02, Vol.29 (7), p.1472-1480 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3032637 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); ProQuest Central UK/Ireland |
| subjects | Allergy and Immunology Animals antibodies Antibodies, Bacterial - blood antigens Applied microbiology Bacterial Proteins - immunology Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Cell Proliferation cell-mediated immunity Chlamydia Chlamydia Infections - immunology Chlamydia Infections - prevention & control Chlamydia trachomatis Chlamydia trachomatis - immunology Delivery Female Fundamental and applied biological sciences. Psychology immune response Immune system Immunity Immunity, Cellular Immunity, Humoral Immunization immunoglobulin A Immunoglobulin A - blood Immunoglobulin A - immunology immunoglobulin G Immunoglobulin G - blood Immunoglobulin G - immunology Immunologic Memory Infections Injections, Intramuscular Membrane Proteins - immunology Mice Mice, Inbred C57BL Microbiology Miscellaneous mucosa mucosal immunity Porins - immunology Sexually transmitted diseases Spleen - immunology STD T-lymphocytes Th1 Cells - immunology Vaccine Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vagina - immunology Vibrio cholerae Vibrio cholerae - immunology Virus Shedding |
| title | Induction of immune memory by a multisubunit chlamydial vaccine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T14%3A35%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Induction%20of%20immune%20memory%20by%20a%20multisubunit%20chlamydial%20vaccine&rft.jtitle=Vaccine&rft.au=Eko,%20F.O&rft.date=2011-02-04&rft.volume=29&rft.issue=7&rft.spage=1472&rft.epage=1480&rft.pages=1472-1480&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/j.vaccine.2010.12.024&rft_dat=%3Cproquest_pubme%3E3218575421%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1498110618&rft_id=info:pmid/21184858&rft_els_id=S0264410X10017627&rfr_iscdi=true |