A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases
Several recent discoveries of the hallmark features of programmed cell death (PCD) in Plasmodium falciparum have presented the possibility of revealing novel targets for antimalarial therapy. Using a combination of cell-based assays, flow cytometry and fluorescence microscopy, we detected features i...
Gespeichert in:
| Veröffentlicht in: | Cell death & disease 2010-02, Vol.1 (2), p.e26-e26 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e26 |
|---|---|
| container_issue | 2 |
| container_start_page | e26 |
| container_title | Cell death & disease |
| container_volume | 1 |
| creator | Ch'ng, J-H Kotturi, S R Chong, A G-L Lear, M J Tan, K S-W |
| description | Several recent discoveries of the hallmark features of programmed cell death (PCD) in
Plasmodium falciparum
have presented the possibility of revealing novel targets for antimalarial therapy. Using a combination of cell-based assays, flow cytometry and fluorescence microscopy, we detected features including mitochondrial dysregulation, activation of cysteine proteases and
in situ
DNA fragmentation in parasites induced with chloroquine (CQ) and staurosporine (ST). The use of the pan-caspase inhibitor, z-Val-Ala-Asp-fmk (zVAD), and the mitochondria outer membrane permeabilization (MOMP) inhibitor, 4-hydroxy-tamoxifen, enabled the characterization of a novel CQ-induced pathway linking cysteine protease activation to downstream mitochondrial dysregulation, amplified protease activity and DNA fragmentation. The PCD features were observed only at high (
μ
M) concentrations of CQ. The use of a new synthetic coumarin-labeled chloroquine (CM-CQ) showed that these features may be associated with concentration-dependent differences in drug localization. By further using cysteine protease inhibitors z-Asp-Glu-Val-Asp-fmk (zDEVD), z-Phe-Ala-fmk (zFA), z-Phe-Phe-fmk (zFF), z-Leu-Leu-Leu-fmk (zLLL), E64d and CA-074, we were able to implicate clan CA cysteine proteases in CQ-mediated PCD. Finally, CQ induction of two CQ-resistant parasite strains, 7G8 and K1, reveals the existence of PCD features in these parasites, the extent of which was less than 3D7. The use of the chemoreversal agent verapamil implicates the parasite digestive vacuole in mediating CQ-induced PCD. |
| doi_str_mv | 10.1038/cddis.2010.2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3032337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1790935752</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-244162960a52f45838d53c4a65b73e2212128a3c35be472d11769fff1cffa7d13</originalsourceid><addsrcrecordid>eNptkktv1DAQxyMEolXpjTOyxIUDKX7ldUFarSggVYIDnK2JM9l1lcTBdor2E_E1O2FLtSBsya_5-T8z9mTZS8GvBFf1O9t1Ll5JTnv5JDuXXItc13Xz9GR9ll3GeMupKcVlUT7PzqRQpS6VPs9-bdgc_C7AOGLHLA4D6xDSns00_IQDcxNLe2QjDBAc0HGA6BKyrwPE0XduGVkPg3VkoOUeItvhhAEG1pPOEjAy39P1kRQcTAxmPycfXWTtkhhN5NdBIuftgdmBiO2G2UNM6CZcY0sIEeOL7Bm5iXj5MF9k368_fNt-ym--fPy83dzkVtcy5VJrUcqm5FDIXhe1qrtCWQ1l0VYKpRTUa1BWFS3qSnZCVGXT972wfQ9VJ9RF9v6oOy8tRWZxSpSLmYMbIRyMB2f-tkxub3b-ziiupFIVCbx5EAj-x4IxmdHF9V1hQr9EI6qGN6qoCkno63_QW7-EidIjqi4LpbVsiHp7pGzwMQbsH4MR3KxFYH4XgVmLwKyir04TeIT_fDkB-RGIZJp2GE68_k_wHuJsv_s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1786534429</pqid></control><display><type>article</type><title>A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases</title><source>PubMed Central Free</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Ch'ng, J-H ; Kotturi, S R ; Chong, A G-L ; Lear, M J ; Tan, K S-W</creator><creatorcontrib>Ch'ng, J-H ; Kotturi, S R ; Chong, A G-L ; Lear, M J ; Tan, K S-W</creatorcontrib><description>Several recent discoveries of the hallmark features of programmed cell death (PCD) in
Plasmodium falciparum
have presented the possibility of revealing novel targets for antimalarial therapy. Using a combination of cell-based assays, flow cytometry and fluorescence microscopy, we detected features including mitochondrial dysregulation, activation of cysteine proteases and
in situ
DNA fragmentation in parasites induced with chloroquine (CQ) and staurosporine (ST). The use of the pan-caspase inhibitor, z-Val-Ala-Asp-fmk (zVAD), and the mitochondria outer membrane permeabilization (MOMP) inhibitor, 4-hydroxy-tamoxifen, enabled the characterization of a novel CQ-induced pathway linking cysteine protease activation to downstream mitochondrial dysregulation, amplified protease activity and DNA fragmentation. The PCD features were observed only at high (
μ
M) concentrations of CQ. The use of a new synthetic coumarin-labeled chloroquine (CM-CQ) showed that these features may be associated with concentration-dependent differences in drug localization. By further using cysteine protease inhibitors z-Asp-Glu-Val-Asp-fmk (zDEVD), z-Phe-Ala-fmk (zFA), z-Phe-Phe-fmk (zFF), z-Leu-Leu-Leu-fmk (zLLL), E64d and CA-074, we were able to implicate clan CA cysteine proteases in CQ-mediated PCD. Finally, CQ induction of two CQ-resistant parasite strains, 7G8 and K1, reveals the existence of PCD features in these parasites, the extent of which was less than 3D7. The use of the chemoreversal agent verapamil implicates the parasite digestive vacuole in mediating CQ-induced PCD.</description><identifier>ISSN: 2041-4889</identifier><identifier>EISSN: 2041-4889</identifier><identifier>DOI: 10.1038/cddis.2010.2</identifier><identifier>PMID: 21364634</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154/555 ; 631/80/82/23 ; 692/699/255/1629 ; Animals ; Antibodies ; Apoptosis - drug effects ; Biochemistry ; Biomedical and Life Sciences ; Calcium - metabolism ; Cell Biology ; Cell Culture ; Chloroquine - pharmacology ; Cysteine Proteases - metabolism ; DNA - metabolism ; DNA Fragmentation - drug effects ; Dose-Response Relationship, Drug ; Fluorescent Dyes - metabolism ; Immunology ; Intracellular Space - drug effects ; Intracellular Space - metabolism ; Life Sciences ; Malaria - parasitology ; Mammals - metabolism ; Membrane Potential, Mitochondrial - drug effects ; Models, Biological ; Necrosis ; Original ; original-article ; Parasites - cytology ; Parasites - drug effects ; Parasites - enzymology ; Plasmodium falciparum ; Plasmodium falciparum - cytology ; Plasmodium falciparum - drug effects ; Plasmodium falciparum - enzymology ; Protozoan Proteins - metabolism ; Reproducibility of Results ; Signal Transduction - drug effects ; Staining and Labeling ; Time Factors</subject><ispartof>Cell death & disease, 2010-02, Vol.1 (2), p.e26-e26</ispartof><rights>The Author(s) 2010</rights><rights>Copyright Nature Publishing Group Feb 2010</rights><rights>Copyright © 2010 Macmillan Publishers Limited 2010 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-244162960a52f45838d53c4a65b73e2212128a3c35be472d11769fff1cffa7d13</citedby><cites>FETCH-LOGICAL-c482t-244162960a52f45838d53c4a65b73e2212128a3c35be472d11769fff1cffa7d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032337/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032337/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21364634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ch'ng, J-H</creatorcontrib><creatorcontrib>Kotturi, S R</creatorcontrib><creatorcontrib>Chong, A G-L</creatorcontrib><creatorcontrib>Lear, M J</creatorcontrib><creatorcontrib>Tan, K S-W</creatorcontrib><title>A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases</title><title>Cell death & disease</title><addtitle>Cell Death Dis</addtitle><addtitle>Cell Death Dis</addtitle><description>Several recent discoveries of the hallmark features of programmed cell death (PCD) in
Plasmodium falciparum
have presented the possibility of revealing novel targets for antimalarial therapy. Using a combination of cell-based assays, flow cytometry and fluorescence microscopy, we detected features including mitochondrial dysregulation, activation of cysteine proteases and
in situ
DNA fragmentation in parasites induced with chloroquine (CQ) and staurosporine (ST). The use of the pan-caspase inhibitor, z-Val-Ala-Asp-fmk (zVAD), and the mitochondria outer membrane permeabilization (MOMP) inhibitor, 4-hydroxy-tamoxifen, enabled the characterization of a novel CQ-induced pathway linking cysteine protease activation to downstream mitochondrial dysregulation, amplified protease activity and DNA fragmentation. The PCD features were observed only at high (
μ
M) concentrations of CQ. The use of a new synthetic coumarin-labeled chloroquine (CM-CQ) showed that these features may be associated with concentration-dependent differences in drug localization. By further using cysteine protease inhibitors z-Asp-Glu-Val-Asp-fmk (zDEVD), z-Phe-Ala-fmk (zFA), z-Phe-Phe-fmk (zFF), z-Leu-Leu-Leu-fmk (zLLL), E64d and CA-074, we were able to implicate clan CA cysteine proteases in CQ-mediated PCD. Finally, CQ induction of two CQ-resistant parasite strains, 7G8 and K1, reveals the existence of PCD features in these parasites, the extent of which was less than 3D7. The use of the chemoreversal agent verapamil implicates the parasite digestive vacuole in mediating CQ-induced PCD.</description><subject>631/154/555</subject><subject>631/80/82/23</subject><subject>692/699/255/1629</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Apoptosis - drug effects</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium - metabolism</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Chloroquine - pharmacology</subject><subject>Cysteine Proteases - metabolism</subject><subject>DNA - metabolism</subject><subject>DNA Fragmentation - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Immunology</subject><subject>Intracellular Space - drug effects</subject><subject>Intracellular Space - metabolism</subject><subject>Life Sciences</subject><subject>Malaria - parasitology</subject><subject>Mammals - metabolism</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Models, Biological</subject><subject>Necrosis</subject><subject>Original</subject><subject>original-article</subject><subject>Parasites - cytology</subject><subject>Parasites - drug effects</subject><subject>Parasites - enzymology</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - cytology</subject><subject>Plasmodium falciparum - drug effects</subject><subject>Plasmodium falciparum - enzymology</subject><subject>Protozoan Proteins - metabolism</subject><subject>Reproducibility of Results</subject><subject>Signal Transduction - drug effects</subject><subject>Staining and Labeling</subject><subject>Time Factors</subject><issn>2041-4889</issn><issn>2041-4889</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkktv1DAQxyMEolXpjTOyxIUDKX7ldUFarSggVYIDnK2JM9l1lcTBdor2E_E1O2FLtSBsya_5-T8z9mTZS8GvBFf1O9t1Ll5JTnv5JDuXXItc13Xz9GR9ll3GeMupKcVlUT7PzqRQpS6VPs9-bdgc_C7AOGLHLA4D6xDSns00_IQDcxNLe2QjDBAc0HGA6BKyrwPE0XduGVkPg3VkoOUeItvhhAEG1pPOEjAy39P1kRQcTAxmPycfXWTtkhhN5NdBIuftgdmBiO2G2UNM6CZcY0sIEeOL7Bm5iXj5MF9k368_fNt-ym--fPy83dzkVtcy5VJrUcqm5FDIXhe1qrtCWQ1l0VYKpRTUa1BWFS3qSnZCVGXT972wfQ9VJ9RF9v6oOy8tRWZxSpSLmYMbIRyMB2f-tkxub3b-ziiupFIVCbx5EAj-x4IxmdHF9V1hQr9EI6qGN6qoCkno63_QW7-EidIjqi4LpbVsiHp7pGzwMQbsH4MR3KxFYH4XgVmLwKyir04TeIT_fDkB-RGIZJp2GE68_k_wHuJsv_s</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Ch'ng, J-H</creator><creator>Kotturi, S R</creator><creator>Chong, A G-L</creator><creator>Lear, M J</creator><creator>Tan, K S-W</creator><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7TO</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20100201</creationdate><title>A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases</title><author>Ch'ng, J-H ; Kotturi, S R ; Chong, A G-L ; Lear, M J ; Tan, K S-W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-244162960a52f45838d53c4a65b73e2212128a3c35be472d11769fff1cffa7d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>631/154/555</topic><topic>631/80/82/23</topic><topic>692/699/255/1629</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Apoptosis - drug effects</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium - metabolism</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Chloroquine - pharmacology</topic><topic>Cysteine Proteases - metabolism</topic><topic>DNA - metabolism</topic><topic>DNA Fragmentation - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Immunology</topic><topic>Intracellular Space - drug effects</topic><topic>Intracellular Space - metabolism</topic><topic>Life Sciences</topic><topic>Malaria - parasitology</topic><topic>Mammals - metabolism</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Models, Biological</topic><topic>Necrosis</topic><topic>Original</topic><topic>original-article</topic><topic>Parasites - cytology</topic><topic>Parasites - drug effects</topic><topic>Parasites - enzymology</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - cytology</topic><topic>Plasmodium falciparum - drug effects</topic><topic>Plasmodium falciparum - enzymology</topic><topic>Protozoan Proteins - metabolism</topic><topic>Reproducibility of Results</topic><topic>Signal Transduction - drug effects</topic><topic>Staining and Labeling</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ch'ng, J-H</creatorcontrib><creatorcontrib>Kotturi, S R</creatorcontrib><creatorcontrib>Chong, A G-L</creatorcontrib><creatorcontrib>Lear, M J</creatorcontrib><creatorcontrib>Tan, K S-W</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell death & disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ch'ng, J-H</au><au>Kotturi, S R</au><au>Chong, A G-L</au><au>Lear, M J</au><au>Tan, K S-W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases</atitle><jtitle>Cell death & disease</jtitle><stitle>Cell Death Dis</stitle><addtitle>Cell Death Dis</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>1</volume><issue>2</issue><spage>e26</spage><epage>e26</epage><pages>e26-e26</pages><issn>2041-4889</issn><eissn>2041-4889</eissn><abstract>Several recent discoveries of the hallmark features of programmed cell death (PCD) in
Plasmodium falciparum
have presented the possibility of revealing novel targets for antimalarial therapy. Using a combination of cell-based assays, flow cytometry and fluorescence microscopy, we detected features including mitochondrial dysregulation, activation of cysteine proteases and
in situ
DNA fragmentation in parasites induced with chloroquine (CQ) and staurosporine (ST). The use of the pan-caspase inhibitor, z-Val-Ala-Asp-fmk (zVAD), and the mitochondria outer membrane permeabilization (MOMP) inhibitor, 4-hydroxy-tamoxifen, enabled the characterization of a novel CQ-induced pathway linking cysteine protease activation to downstream mitochondrial dysregulation, amplified protease activity and DNA fragmentation. The PCD features were observed only at high (
μ
M) concentrations of CQ. The use of a new synthetic coumarin-labeled chloroquine (CM-CQ) showed that these features may be associated with concentration-dependent differences in drug localization. By further using cysteine protease inhibitors z-Asp-Glu-Val-Asp-fmk (zDEVD), z-Phe-Ala-fmk (zFA), z-Phe-Phe-fmk (zFF), z-Leu-Leu-Leu-fmk (zLLL), E64d and CA-074, we were able to implicate clan CA cysteine proteases in CQ-mediated PCD. Finally, CQ induction of two CQ-resistant parasite strains, 7G8 and K1, reveals the existence of PCD features in these parasites, the extent of which was less than 3D7. The use of the chemoreversal agent verapamil implicates the parasite digestive vacuole in mediating CQ-induced PCD.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>21364634</pmid><doi>10.1038/cddis.2010.2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2041-4889 |
| ispartof | Cell death & disease, 2010-02, Vol.1 (2), p.e26-e26 |
| issn | 2041-4889 2041-4889 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3032337 |
| source | PubMed Central Free; MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals |
| subjects | 631/154/555 631/80/82/23 692/699/255/1629 Animals Antibodies Apoptosis - drug effects Biochemistry Biomedical and Life Sciences Calcium - metabolism Cell Biology Cell Culture Chloroquine - pharmacology Cysteine Proteases - metabolism DNA - metabolism DNA Fragmentation - drug effects Dose-Response Relationship, Drug Fluorescent Dyes - metabolism Immunology Intracellular Space - drug effects Intracellular Space - metabolism Life Sciences Malaria - parasitology Mammals - metabolism Membrane Potential, Mitochondrial - drug effects Models, Biological Necrosis Original original-article Parasites - cytology Parasites - drug effects Parasites - enzymology Plasmodium falciparum Plasmodium falciparum - cytology Plasmodium falciparum - drug effects Plasmodium falciparum - enzymology Protozoan Proteins - metabolism Reproducibility of Results Signal Transduction - drug effects Staining and Labeling Time Factors |
| title | A programmed cell death pathway in the malaria parasite Plasmodium falciparum has general features of mammalian apoptosis but is mediated by clan CA cysteine proteases |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T20%3A01%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20programmed%20cell%20death%20pathway%20in%20the%20malaria%20parasite%20Plasmodium%20falciparum%20has%20general%20features%20of%20mammalian%20apoptosis%20but%20is%20mediated%20by%20clan%20CA%20cysteine%20proteases&rft.jtitle=Cell%20death%20&%20disease&rft.au=Ch'ng,%20J-H&rft.date=2010-02-01&rft.volume=1&rft.issue=2&rft.spage=e26&rft.epage=e26&rft.pages=e26-e26&rft.issn=2041-4889&rft.eissn=2041-4889&rft_id=info:doi/10.1038/cddis.2010.2&rft_dat=%3Cproquest_pubme%3E1790935752%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1786534429&rft_id=info:pmid/21364634&rfr_iscdi=true |