Demonstration of LanthaScreen™ TR-FRET-based nuclear receptor coactivator recruitment assay using PHERAstar, a multi-detection HTS microplate reader
An attempt was made to demonstrate the possibility of performing LanthaScreen™ TR-FRET based nuclear receptor coactivator recruitment assay using PHERAstar, a multi-detection HTS microplate reader. LanthaScreen™ nuclear receptor coactivator recruitment assay (M/s Invitrogen corporation, USA) was per...
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| Veröffentlicht in: | Indian journal of pharmacology 2008-03, Vol.40 (2), p.89-90 |
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| container_title | Indian journal of pharmacology |
| container_volume | 40 |
| creator | D'Souza, Prashanth C.G Ally, Nafisa Agarwal, Amit Rani, Padmaja Das, Meenakshi Sarma, Dolly |
| description | An attempt was made to demonstrate the possibility of performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assay using PHERAstar, a multi-detection HTS microplate
reader. LanthaScreen™ nuclear receptor coactivator recruitment
assay (M/s Invitrogen corporation, USA) was performed using PPAR-gamma
receptor preparation in the agonist mode. TR-FRET measurements were
done on PHERAstar, a multimode microplate reader (BMG LABTECH,
Germany). The Lanthascreen PPAR gamma coactivator recruitment assay was
successfully performed in the PHERAstar, multimode microplate reader.
This was evidenced by an assay robustness score (Z′) of 0.71. The
current work demonstrates the suitability of using PHERAstar, a
multi-detection HTS microplate reader.for performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assays. |
| doi_str_mv | 10.4103/0253-7613.41046 |
| format | Article |
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LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assay using PHERAstar, a multi-detection HTS microplate
reader. LanthaScreen™ nuclear receptor coactivator recruitment
assay (M/s Invitrogen corporation, USA) was performed using PPAR-gamma
receptor preparation in the agonist mode. TR-FRET measurements were
done on PHERAstar, a multimode microplate reader (BMG LABTECH,
Germany). The Lanthascreen PPAR gamma coactivator recruitment assay was
successfully performed in the PHERAstar, multimode microplate reader.
This was evidenced by an assay robustness score (Z′) of 0.71. The
current work demonstrates the suitability of using PHERAstar, a
multi-detection HTS microplate reader.for performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assays.</description><identifier>ISSN: 0253-7613</identifier><identifier>EISSN: 1998-3751</identifier><identifier>DOI: 10.4103/0253-7613.41046</identifier><identifier>PMID: 21279174</identifier><language>eng</language><publisher>India: Medknow Publications on behalf of Indian Pharmacological Society</publisher><subject>Assaying ; Assaying apparatus ; Case Report ; Cell physiology ; DNA binding proteins ; HTS, LanthaScreen TM nuclear receptor coregulator assays, nuclear receptors, PHERAstar, time resolved-fluorescence resonance energy transfer ; Methods ; Pharmacology, Experimental ; Properties</subject><ispartof>Indian journal of pharmacology, 2008-03, Vol.40 (2), p.89-90</ispartof><rights>Copyright 2008 Indian Journal of Pharmacology.</rights><rights>COPYRIGHT 2008 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications Mar/Apr 2008</rights><rights>Indian Journal of Pharmacology 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b563t-9ed97cc67ebdbb5e86c622af68866f17b00028b4b602aef5729de1b828683f43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025134/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025134/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,79426</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21279174$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>D'Souza, Prashanth C.G</creatorcontrib><creatorcontrib>Ally, Nafisa</creatorcontrib><creatorcontrib>Agarwal, Amit</creatorcontrib><creatorcontrib>Rani, Padmaja</creatorcontrib><creatorcontrib>Das, Meenakshi</creatorcontrib><creatorcontrib>Sarma, Dolly</creatorcontrib><title>Demonstration of LanthaScreen™ TR-FRET-based nuclear receptor coactivator recruitment assay using PHERAstar, a multi-detection HTS microplate reader</title><title>Indian journal of pharmacology</title><addtitle>Indian J Pharmacol</addtitle><description>An attempt was made to demonstrate the possibility of performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assay using PHERAstar, a multi-detection HTS microplate
reader. LanthaScreen™ nuclear receptor coactivator recruitment
assay (M/s Invitrogen corporation, USA) was performed using PPAR-gamma
receptor preparation in the agonist mode. TR-FRET measurements were
done on PHERAstar, a multimode microplate reader (BMG LABTECH,
Germany). The Lanthascreen PPAR gamma coactivator recruitment assay was
successfully performed in the PHERAstar, multimode microplate reader.
This was evidenced by an assay robustness score (Z′) of 0.71. The
current work demonstrates the suitability of using PHERAstar, a
multi-detection HTS microplate reader.for performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assays.</description><subject>Assaying</subject><subject>Assaying apparatus</subject><subject>Case Report</subject><subject>Cell physiology</subject><subject>DNA binding proteins</subject><subject>HTS, LanthaScreen TM nuclear receptor coregulator assays, nuclear receptors, PHERAstar, time resolved-fluorescence resonance energy transfer</subject><subject>Methods</subject><subject>Pharmacology, Experimental</subject><subject>Properties</subject><issn>0253-7613</issn><issn>1998-3751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptksFu1DAQhiMEokvhzA1ZcOBC2jhOHPtSaVW2LNJKoO3eLceZ7LpK7MV2KvXOM_AAPBpPgtMtgaJVIsUef_PPePInyWucnRU4I-dZXpK0opiM24I-SWaYc5aSqsRPk9l0epK88P4mi_uC0-fJSY7ziuOqmCU_PkJvjQ9OBm0Nsi1aSRN28lo5APPr-0-0WadX68UmraWHBplBdSAdcqBgH6xDykoV9K0c1zHoBh16MAFJ7-UdGrw2W_R1uVjPfZDuA5KoH7qg0wYCqPuSy8016rVydt_JAFFDNuBeJs9a2Xl49fA9TTZXi83lMl19-fT5cr5K65KSkHJoeKUUraBu6roERhXNc9lSxihtcVVn8c6sLmqa5RLassp5A7hmOaOMtAU5TS4Osvuh7qFRsXEnO7F3upfuTlipxeMTo3dia28FiaPEZBR4_yDg7LcBfBC99gq6ThqwgxesiJUYLspIvv2PvLGDM_FyAvOSVUVBeITeHaCt7EBo09pYVY2SYo4rzrM8K6pIpUeoLRiILVoDrY7hR_zZET4-DcTBH004PyTEv-K9g3aaCM7E6DsxOkuMzhL3vosZb_4d5MT_MdrfHmptO21gIpTTUkzBXXwzlhFMfgOddeWc</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>D'Souza, Prashanth C.G</creator><creator>Ally, Nafisa</creator><creator>Agarwal, Amit</creator><creator>Rani, Padmaja</creator><creator>Das, Meenakshi</creator><creator>Sarma, Dolly</creator><general>Medknow Publications on behalf of Indian Pharmacological Society</general><general>Medknow Publications and Media Pvt. 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LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assay using PHERAstar, a multi-detection HTS microplate
reader. LanthaScreen™ nuclear receptor coactivator recruitment
assay (M/s Invitrogen corporation, USA) was performed using PPAR-gamma
receptor preparation in the agonist mode. TR-FRET measurements were
done on PHERAstar, a multimode microplate reader (BMG LABTECH,
Germany). The Lanthascreen PPAR gamma coactivator recruitment assay was
successfully performed in the PHERAstar, multimode microplate reader.
This was evidenced by an assay robustness score (Z′) of 0.71. The
current work demonstrates the suitability of using PHERAstar, a
multi-detection HTS microplate reader.for performing
LanthaScreen™ TR-FRET based nuclear receptor coactivator
recruitment assays.</abstract><cop>India</cop><pub>Medknow Publications on behalf of Indian Pharmacological Society</pub><pmid>21279174</pmid><doi>10.4103/0253-7613.41046</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0253-7613 |
| ispartof | Indian journal of pharmacology, 2008-03, Vol.40 (2), p.89-90 |
| issn | 0253-7613 1998-3751 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3025134 |
| source | PubMed Central Open Access; Bioline International; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Assaying Assaying apparatus Case Report Cell physiology DNA binding proteins HTS, LanthaScreen TM nuclear receptor coregulator assays, nuclear receptors, PHERAstar, time resolved-fluorescence resonance energy transfer Methods Pharmacology, Experimental Properties |
| title | Demonstration of LanthaScreen™ TR-FRET-based nuclear receptor coactivator recruitment assay using PHERAstar, a multi-detection HTS microplate reader |
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