Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis
50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic r...
Gespeichert in:
| Veröffentlicht in: | BioMed research international 2011-01, Vol.2011 (2011), p.1-9 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9 |
|---|---|
| container_issue | 2011 |
| container_start_page | 1 |
| container_title | BioMed research international |
| container_volume | 2011 |
| creator | Fernandes, Julio C. Dai, Kerong Zhang, Xiaoling Winnik, Françoise M. Qiu, Xingping Tran, Covi Wang, Huijie Shi, Qin Benderdour, Mohamed |
| description | 50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic rats. Our results demonstrated that the fluorescence intensity of DsRed detected was 5 to 12 times more in the right soleus muscle and in the right gastro muscle than other tissue sections. β-galactosidase gene expression with X-gal substrate and folate-chitosan-plasmid nanoparticles showed best coloration in the soleus muscle. Treated arthritic animals also showed a significant decrease in paw swelling and IL-1β and PGE2 concentration in serum compared to untreated rats. This study demonstrated that a nonviral gene therapeutic approach using hydrodynamic delivery could help transfect more efficiently folate-chitosan-DNA nanoparticles in vitro/in vivo and could decrease inflammation in arthritic rats. |
| doi_str_mv | 10.1155/2011/148763 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3022186</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A266629971</galeid><sourcerecordid>A266629971</sourcerecordid><originalsourceid>FETCH-LOGICAL-c569t-65899597a8e69d1abf5e99bd99c012036a861b2c78b43d0ede965de07fa617903</originalsourceid><addsrcrecordid>eNqFktFrFDEQxhdRbK0--aws-qZsm2Q32eRFWK7WFkoF0eeQTWbvcuwlZ5K9cv-9KVsPCweSh4TMb77JTL6ieIvROcaUXhCE8QVueMvqZ8UpxhhVLaH4-eHc1CfFqxjXCOGWM_GyOCGYtA2h_LTor_cmeLN3amN1eQmj3UHYl34oFyubfFSuuvKjSlBd3nXlnXJ-q0KyeoRYWlf-UCmW9zatys6sp51yqbpxZtJgyi6kVbDJxtfFi0GNEd487mfFr6uvPxfX1e33bzeL7rbSlIlUMcqFoKJVHJgwWPUDBSF6I4RGmKCaKc5wT3TL-6Y2CAwIRg2gdlAMtwLVZ8WXWXc79RswGlwKapTbYDcq7KVXVj6NOLuSS7-TNSIEc5YFPjwKBP97gpjk2k_B5TdLThnJIxNthj7O0FKNIK0bfNbSGxu17AhjjAjR4kxVR6glOMiFvYPB5usn_PkRPi8D-V-OJnyeE3TwMQYYDn1iJB9sIR9sIWdbZPr9v6M5sH99kIFPM7Cyzqh7-x-1dzMMGYFBHeCmEazh9R_FYse6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>856227497</pqid></control><display><type>article</type><title>Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Fernandes, Julio C. ; Dai, Kerong ; Zhang, Xiaoling ; Winnik, Françoise M. ; Qiu, Xingping ; Tran, Covi ; Wang, Huijie ; Shi, Qin ; Benderdour, Mohamed</creator><contributor>Gualillo, Oreste</contributor><creatorcontrib>Fernandes, Julio C. ; Dai, Kerong ; Zhang, Xiaoling ; Winnik, Françoise M. ; Qiu, Xingping ; Tran, Covi ; Wang, Huijie ; Shi, Qin ; Benderdour, Mohamed ; Gualillo, Oreste</creatorcontrib><description>50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic rats. Our results demonstrated that the fluorescence intensity of DsRed detected was 5 to 12 times more in the right soleus muscle and in the right gastro muscle than other tissue sections. β-galactosidase gene expression with X-gal substrate and folate-chitosan-plasmid nanoparticles showed best coloration in the soleus muscle. Treated arthritic animals also showed a significant decrease in paw swelling and IL-1β and PGE2 concentration in serum compared to untreated rats. This study demonstrated that a nonviral gene therapeutic approach using hydrodynamic delivery could help transfect more efficiently folate-chitosan-DNA nanoparticles in vitro/in vivo and could decrease inflammation in arthritic rats.</description><identifier>ISSN: 1110-7243</identifier><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 1110-7251</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2011/148763</identifier><identifier>PMID: 21274258</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject><![CDATA[Analysis of Variance ; Animals ; Arthritis ; Arthritis, Experimental - genetics ; Arthritis, Experimental - metabolism ; Arthritis, Experimental - therapy ; beta-Galactosidase - biosynthesis ; beta-Galactosidase - genetics ; Cancer ; Care and treatment ; Chitin ; Chitosan - administration & dosage ; Dinoprostone - metabolism ; Disease Models, Animal ; DNA - administration & dosage ; DNA - genetics ; Drug Delivery Systems - methods ; Female ; Folic Acid - administration & dosage ; Freund's Adjuvant - administration & dosage ; Gene expression ; Gene therapy ; Genetic aspects ; Histocytochemistry ; Humans ; Injections, Intravenous ; Interleukin 1 Receptor Antagonist Protein - genetics ; Interleukin 1 Receptor Antagonist Protein - metabolism ; Interleukin-1beta - metabolism ; Medical research ; Muscle, Skeletal - metabolism ; Nanoconjugates - administration & dosage ; Nanoconjugates - chemistry ; Nanoparticles ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Physiological aspects ; Proteins ; Rats ; Rats, Inbred Lew ; Rodents ; Tarsus, Animal - pathology ; Tissue Distribution]]></subject><ispartof>BioMed research international, 2011-01, Vol.2011 (2011), p.1-9</ispartof><rights>Copyright © 2011 Qin Shi et al.</rights><rights>COPYRIGHT 2011 John Wiley & Sons, Inc.</rights><rights>Copyright © 2011 Qin Shi et al. Qin Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2011 Qin Shi et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-65899597a8e69d1abf5e99bd99c012036a861b2c78b43d0ede965de07fa617903</citedby><cites>FETCH-LOGICAL-c569t-65899597a8e69d1abf5e99bd99c012036a861b2c78b43d0ede965de07fa617903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022186/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022186/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21274258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gualillo, Oreste</contributor><creatorcontrib>Fernandes, Julio C.</creatorcontrib><creatorcontrib>Dai, Kerong</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><creatorcontrib>Winnik, Françoise M.</creatorcontrib><creatorcontrib>Qiu, Xingping</creatorcontrib><creatorcontrib>Tran, Covi</creatorcontrib><creatorcontrib>Wang, Huijie</creatorcontrib><creatorcontrib>Shi, Qin</creatorcontrib><creatorcontrib>Benderdour, Mohamed</creatorcontrib><title>Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis</title><title>BioMed research international</title><addtitle>J Biomed Biotechnol</addtitle><description>50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic rats. Our results demonstrated that the fluorescence intensity of DsRed detected was 5 to 12 times more in the right soleus muscle and in the right gastro muscle than other tissue sections. β-galactosidase gene expression with X-gal substrate and folate-chitosan-plasmid nanoparticles showed best coloration in the soleus muscle. Treated arthritic animals also showed a significant decrease in paw swelling and IL-1β and PGE2 concentration in serum compared to untreated rats. This study demonstrated that a nonviral gene therapeutic approach using hydrodynamic delivery could help transfect more efficiently folate-chitosan-DNA nanoparticles in vitro/in vivo and could decrease inflammation in arthritic rats.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Arthritis</subject><subject>Arthritis, Experimental - genetics</subject><subject>Arthritis, Experimental - metabolism</subject><subject>Arthritis, Experimental - therapy</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>beta-Galactosidase - genetics</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Chitin</subject><subject>Chitosan - administration & dosage</subject><subject>Dinoprostone - metabolism</subject><subject>Disease Models, Animal</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>Drug Delivery Systems - methods</subject><subject>Female</subject><subject>Folic Acid - administration & dosage</subject><subject>Freund's Adjuvant - administration & dosage</subject><subject>Gene expression</subject><subject>Gene therapy</subject><subject>Genetic aspects</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Injections, Intravenous</subject><subject>Interleukin 1 Receptor Antagonist Protein - genetics</subject><subject>Interleukin 1 Receptor Antagonist Protein - metabolism</subject><subject>Interleukin-1beta - metabolism</subject><subject>Medical research</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Nanoconjugates - administration & dosage</subject><subject>Nanoconjugates - chemistry</subject><subject>Nanoparticles</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Rodents</subject><subject>Tarsus, Animal - pathology</subject><subject>Tissue Distribution</subject><issn>1110-7243</issn><issn>2314-6133</issn><issn>1110-7251</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFktFrFDEQxhdRbK0--aws-qZsm2Q32eRFWK7WFkoF0eeQTWbvcuwlZ5K9cv-9KVsPCweSh4TMb77JTL6ieIvROcaUXhCE8QVueMvqZ8UpxhhVLaH4-eHc1CfFqxjXCOGWM_GyOCGYtA2h_LTor_cmeLN3amN1eQmj3UHYl34oFyubfFSuuvKjSlBd3nXlnXJ-q0KyeoRYWlf-UCmW9zatys6sp51yqbpxZtJgyi6kVbDJxtfFi0GNEd487mfFr6uvPxfX1e33bzeL7rbSlIlUMcqFoKJVHJgwWPUDBSF6I4RGmKCaKc5wT3TL-6Y2CAwIRg2gdlAMtwLVZ8WXWXc79RswGlwKapTbYDcq7KVXVj6NOLuSS7-TNSIEc5YFPjwKBP97gpjk2k_B5TdLThnJIxNthj7O0FKNIK0bfNbSGxu17AhjjAjR4kxVR6glOMiFvYPB5usn_PkRPi8D-V-OJnyeE3TwMQYYDn1iJB9sIR9sIWdbZPr9v6M5sH99kIFPM7Cyzqh7-x-1dzMMGYFBHeCmEazh9R_FYse6</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Fernandes, Julio C.</creator><creator>Dai, Kerong</creator><creator>Zhang, Xiaoling</creator><creator>Winnik, Françoise M.</creator><creator>Qiu, Xingping</creator><creator>Tran, Covi</creator><creator>Wang, Huijie</creator><creator>Shi, Qin</creator><creator>Benderdour, Mohamed</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20110101</creationdate><title>Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis</title><author>Fernandes, Julio C. ; Dai, Kerong ; Zhang, Xiaoling ; Winnik, Françoise M. ; Qiu, Xingping ; Tran, Covi ; Wang, Huijie ; Shi, Qin ; Benderdour, Mohamed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-65899597a8e69d1abf5e99bd99c012036a861b2c78b43d0ede965de07fa617903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Arthritis</topic><topic>Arthritis, Experimental - genetics</topic><topic>Arthritis, Experimental - metabolism</topic><topic>Arthritis, Experimental - therapy</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>beta-Galactosidase - genetics</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Chitin</topic><topic>Chitosan - administration & dosage</topic><topic>Dinoprostone - metabolism</topic><topic>Disease Models, Animal</topic><topic>DNA - administration & dosage</topic><topic>DNA - genetics</topic><topic>Drug Delivery Systems - methods</topic><topic>Female</topic><topic>Folic Acid - administration & dosage</topic><topic>Freund's Adjuvant - administration & dosage</topic><topic>Gene expression</topic><topic>Gene therapy</topic><topic>Genetic aspects</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Injections, Intravenous</topic><topic>Interleukin 1 Receptor Antagonist Protein - genetics</topic><topic>Interleukin 1 Receptor Antagonist Protein - metabolism</topic><topic>Interleukin-1beta - metabolism</topic><topic>Medical research</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Nanoconjugates - administration & dosage</topic><topic>Nanoconjugates - chemistry</topic><topic>Nanoparticles</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Rodents</topic><topic>Tarsus, Animal - pathology</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes, Julio C.</creatorcontrib><creatorcontrib>Dai, Kerong</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><creatorcontrib>Winnik, Françoise M.</creatorcontrib><creatorcontrib>Qiu, Xingping</creatorcontrib><creatorcontrib>Tran, Covi</creatorcontrib><creatorcontrib>Wang, Huijie</creatorcontrib><creatorcontrib>Shi, Qin</creatorcontrib><creatorcontrib>Benderdour, Mohamed</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes, Julio C.</au><au>Dai, Kerong</au><au>Zhang, Xiaoling</au><au>Winnik, Françoise M.</au><au>Qiu, Xingping</au><au>Tran, Covi</au><au>Wang, Huijie</au><au>Shi, Qin</au><au>Benderdour, Mohamed</au><au>Gualillo, Oreste</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis</atitle><jtitle>BioMed research international</jtitle><addtitle>J Biomed Biotechnol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1110-7243</issn><issn>2314-6133</issn><eissn>1110-7251</eissn><eissn>2314-6141</eissn><abstract>50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic rats. Our results demonstrated that the fluorescence intensity of DsRed detected was 5 to 12 times more in the right soleus muscle and in the right gastro muscle than other tissue sections. β-galactosidase gene expression with X-gal substrate and folate-chitosan-plasmid nanoparticles showed best coloration in the soleus muscle. Treated arthritic animals also showed a significant decrease in paw swelling and IL-1β and PGE2 concentration in serum compared to untreated rats. This study demonstrated that a nonviral gene therapeutic approach using hydrodynamic delivery could help transfect more efficiently folate-chitosan-DNA nanoparticles in vitro/in vivo and could decrease inflammation in arthritic rats.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>21274258</pmid><doi>10.1155/2011/148763</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1110-7243 |
| ispartof | BioMed research international, 2011-01, Vol.2011 (2011), p.1-9 |
| issn | 1110-7243 2314-6133 1110-7251 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3022186 |
| source | MEDLINE; PubMed Central Open Access; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | Analysis of Variance Animals Arthritis Arthritis, Experimental - genetics Arthritis, Experimental - metabolism Arthritis, Experimental - therapy beta-Galactosidase - biosynthesis beta-Galactosidase - genetics Cancer Care and treatment Chitin Chitosan - administration & dosage Dinoprostone - metabolism Disease Models, Animal DNA - administration & dosage DNA - genetics Drug Delivery Systems - methods Female Folic Acid - administration & dosage Freund's Adjuvant - administration & dosage Gene expression Gene therapy Genetic aspects Histocytochemistry Humans Injections, Intravenous Interleukin 1 Receptor Antagonist Protein - genetics Interleukin 1 Receptor Antagonist Protein - metabolism Interleukin-1beta - metabolism Medical research Muscle, Skeletal - metabolism Nanoconjugates - administration & dosage Nanoconjugates - chemistry Nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Physiological aspects Proteins Rats Rats, Inbred Lew Rodents Tarsus, Animal - pathology Tissue Distribution |
| title | Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T17%3A45%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hydrodynamic%20Delivery%20of%20Chitosan-Folate-DNA%20Nanoparticles%20in%20Rats%20with%20Adjuvant-Induced%20Arthritis&rft.jtitle=BioMed%20research%20international&rft.au=Fernandes,%20Julio%20C.&rft.date=2011-01-01&rft.volume=2011&rft.issue=2011&rft.spage=1&rft.epage=9&rft.pages=1-9&rft.issn=1110-7243&rft.eissn=1110-7251&rft_id=info:doi/10.1155/2011/148763&rft_dat=%3Cgale_pubme%3EA266629971%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=856227497&rft_id=info:pmid/21274258&rft_galeid=A266629971&rfr_iscdi=true |